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A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage 总被引:11,自引:0,他引:11
Woudstra EC Gilbert C Fellows J Jansen L Brouwer J Erdjument-Bromage H Tempst P Svejstrup JQ 《Nature》2002,415(6874):929-933
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Carlton JM Angiuoli SV Suh BB Kooij TW Pertea M Silva JC Ermolaeva MD Allen JE Selengut JD Koo HL Peterson JD Pop M Kosack DS Shumway MF Bidwell SL Shallom SJ van Aken SE Riedmuller SB Feldblyum TV Cho JK Quackenbush J Sedegah M Shoaibi A Cummings LM Florens L Yates JR Raine JD Sinden RE Harris MA Cunningham DA Preiser PR Bergman LW Vaidya AB van Lin LH Janse CJ Waters AP Smith HO White OR Salzberg SL Venter JC Fraser CM Hoffman SL Gardner MJ Carucci DJ 《Nature》2002,419(6906):512-519
Species of malaria parasite that infect rodents have long been used as models for malaria disease research. Here we report the whole-genome shotgun sequence of one species, Plasmodium yoelii yoelii, and comparative studies with the genome of the human malaria parasite Plasmodium falciparum clone 3D7. A synteny map of 2,212 P. y. yoelii contiguous DNA sequences (contigs) aligned to 14 P. falciparum chromosomes reveals marked conservation of gene synteny within the body of each chromosome. Of about 5,300 P. falciparum genes, more than 3,300 P. y. yoelii orthologues of predominantly metabolic function were identified. Over 800 copies of a variant antigen gene located in subtelomeric regions were found. This is the first genome sequence of a model eukaryotic parasite, and it provides insight into the use of such systems in the modelling of Plasmodium biology and disease. 相似文献
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Identification of human brain tumour initiating cells 总被引:3,自引:0,他引:3
Singh SK Hawkins C Clarke ID Squire JA Bayani J Hide T Henkelman RM Cusimano MD Dirks PB 《Nature》2004,432(7015):396-401
The cancer stem cell (CSC) hypothesis suggests that neoplastic clones are maintained exclusively by a rare fraction of cells with stem cell properties. Although the existence of CSCs in human leukaemia is established, little evidence exists for CSCs in solid tumours, except for breast cancer. Recently, we prospectively isolated a CD133+ cell subpopulation from human brain tumours that exhibited stem cell properties in vitro. However, the true measures of CSCs are their capacity for self renewal and exact recapitulation of the original tumour. Here we report the development of a xenograft assay that identified human brain tumour initiating cells that initiate tumours in vivo. Only the CD133+ brain tumour fraction contains cells that are capable of tumour initiation in NOD-SCID (non-obese diabetic, severe combined immunodeficient) mouse brains. Injection of as few as 100 CD133+ cells produced a tumour that could be serially transplanted and was a phenocopy of the patient's original tumour, whereas injection of 10(5) CD133- cells engrafted but did not cause a tumour. Thus, the identification of brain tumour initiating cells provides insights into human brain tumour pathogenesis, giving strong support for the CSC hypothesis as the basis for many solid tumours, and establishes a previously unidentified cellular target for more effective cancer therapies. 相似文献