Optimization of the cellular import of functionally active SH2-domain-interacting phosphopeptides

Phosphopeptides interacting with src homology 2 (SH2) domains can activate essential signaling enzymes in vitro. When delivered to cells, they may disrupt protein-protein interactions, thereby influencing intracellular signaling. We showed earlier that phosphopeptides corresponding to the inhibitory motif of Fcγ receptor IIb and a motif of the Grb2-associated binder 1 adaptor protein activate SH2-containing tyrosine phosphatase 2 in vitro. To study the ex vivo effects of these peptides, we have now compared different methods for peptide delivery: (i) permeabilization of the target cells and (ii) the use of cell-permeable vectors, which are potentially able to transport biologically active compounds into B cells. We found octanoyl-Arg₈ to be an optimal carrier for the delivery of phosphopeptides to the cells. With this strategy, the function of cell-permeable SHP-2-binding phosphopeptides was analyzed. These peptides modulated the protein phosphorylation in B cells in a dose- and time-dependent manner. Received 27 July 2006; received after revision 4 September 2006; accepted 18 September 2006     

On the self-association potential of transmembrane tight junction proteins

Tight junctions seal intercellular clefts via membrane-related strands, hence, maintaining important organ functions. We investigated the self-association of strand-forming transmembrane tight junction proteins. The regulatory tight junction protein occludin was differently tagged and cotransfected in eucaryotic cells. These occludins colocalized within the plasma membrane of the same cell, coprecipitated and exhibited fluorescence resonance energy transfer. Differently tagged strand-forming claudin-5 also colocalized in the plasma membrane of the same cell and showed fluorescence resonance energy transfer. This demonstrates self-association in intact cells both of occludin and claudin-5 in one plasma membrane. In search of dimerizing regions of occludin, dimerization of its cytosolic C-terminal coiledcoil domain was identified. In claudin-5, the second extracellular loop was detected as a dimer. Since the transmembrane junctional adhesion molecule also is known to dimerize, the assumption that homodimerization of transmembrane tight junction proteins may serve as a common structural feature in tight junction assembly is supported. Received 6 October 2005; received after revision 14 December 2005; accepted 27 December 2005 †These authors contributed equally to this work.     

中国苏北预先导孔CCSD-PP2片麻岩中锆石的矿物包裹体及其超高压变质作用的证据

采用激光Raman及阴极发光技术,确认中国大陆科学钻探工程预选预先导孔CCSD－PP2几乎所有类型片麻岩中的锆石均保存典型的柯石英包体以及绿辉石、硬玉、石榴石、多硅白云母等矿物包体,表明片麻岩类岩石曾普遍经历了超高压变质作用,该成果对于重新探讨苏鲁超高压变质带的俯冲－折返机制和对中国大陆科学钻探工程的选址有重要的科学意义。     

柴达木山花岗岩锆石SHRIMP定年

锆石SHRIMP定年研究表明,祁连南缘柴达木山花岗岩与超高压榴辉岩是构造演化过程中不同阶段形成的,即加里东早期,该区存在洋壳－陆壳碰撞,形成榴辉岩;加里东晚期,存在陆壳－陆壳的碰撞,形成了该花岗岩体。该岩体的SHRIMP年龄为446Ma。     

音乐的物理学和心理物理学

本书是一本关于音乐物理学的大学教材，被一些美国的大学所选用。作者是一位空间物理学家，音乐物理学和心理物理学的研究是他的爱好和副产品。本书作为导论性的教材，处理在通常意义下的“音乐”中物理系统与生物过程相互的关联和影响，详细地分析了声音的各种模式、物理性质与音乐的主观和心理感受紧密联系。     

GPS在长大隧道控制测量中的应用与实现

建立隧道施工控制网通常采用三角测量法和精密导线法,但这些常规方法受到通视条件、图形条件及地形条件等诸多因素的影响。而基于GPS技术建立的隧道测量控制网具有精度高、布网方便等优点。本论述以三棵树隧道为例,阐述GPS在长大隧道控制测量中的实际应用,以期对该行业工作者起到一定借鉴意义。     

光学薄膜镀制技术的对比研究

本文介绍了两种光学镀膜真空淀积技术——离子辅助淀积和反应离子镀,并详述了近年来的应用及进一步的发展。     

用Bochner—Riesz平均逼近及Hardy求和

设Ｆ∈Ｃ（Ｑｎ），Ｎ∈Ｎ且ＳＲ×（ｎ－１）／２（ｆ）是ｆ的临界阶Ｂｏｃｈｎｅｒ－Ｒｉｅｓｚ平均，求得了（Ｈ，ｑ）逼近的阶的估计：｜｜１／Ｒ∫０＾Ｒ｜ｆ－Ｓｒ＾（ｎ－１）２（ｆ）｜＾ｑｄｒ∞≤ｃ１／Ｒ∫｜ｗ２（ｆ；１／ｒ）｜＾ｑｄｒ Ｒ＞０，其中ｗ２表示二阶连续模，ｑ＞０且Ｃ是常数，同时研究了这类逼近的饱和问题。     

水轮机调速器智能化自完善控制策略

本文提出了智能化自完善控制策略,通过采用“知识库”存贮最佳的知识与经验,根据受控对象当前运行条件及状态,运用相应的分析逻辑及推理功能,实时地调整控制器结构及参数,以实现逐步改善控制系统性能的目的.文中将所论述的控制策略用于水轮机调速器开发研究.