An exonucleolytic activity of human apurinic/apyrimidinic endonuclease on 3' mispaired DNA

Human apurinic/apyrimidinic endonuclease (APE1) is an essential enzyme in DNA base excision repair that cuts the DNA backbone immediately adjacent to the 5' side of abasic sites to facilitate repair synthesis by DNA polymerase beta (ref. 1). Mice lacking the murine homologue of APE1 die at an early embryonic stage. Here we report that APE1 has a DNA exonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules. The efficiency of this activity is inversely proportional to the gap size in DNA. In a base excision repair system reconstituted in vitro, the rejoining of nicked mismatched DNA depended on the presence of APE1, indicating that APE1 may increase the fidelity of base excision repair and may represent a new 3' mispaired DNA repair mechanism. The exonuclease activity of APE1 can remove the anti-HIV nucleoside analogues 3'-azido-3'-deoxythymidine and 2',3'-didehydro-2', 3'-dideoxythymidine from DNA, suggesting that APE1 might have an impact on the therapeutic index of antiviral compounds in this category.     

大电机线棒超宽频带局部放电信号的时频特性研究

利用开发的超宽频带局部放电测试系统,研究了退出运行的300MW汽轮发电机定子线棒和780kW电动机线棒的超宽频带局部放电特性。运用联合时频分析技术,分析了这些放电信号的时频域特征,发现线棒的局部放电时域信号包含着丰富的高频分量,其直线部分和“R”部分的主放电特征峰出现的位置比较相似。同时,还发现随着老化程度的加深,放电时延缩短,在时频联合域上能量密度向高频方向移动。该发现为今后评估电机线棒的绝缘寿命提供了一种新的方法。     

"三个代表"是中国"实践的唯物主义者"的使命

"实践的唯物主义者,即共产主义者","三个代表"的重要思想是对马、恩有关共产主义者使命著名论断的有力坚持和发展,充分体现了共产主义者使命的共性与个性的内在统一,渗透和贯穿了"实践的唯物主义"的丰富而深刻的哲学内涵.共产主义者使命的哲学依据,是基于实践辩证地把握和对待现存世界、社会历史及人生的"实践的唯物主义".     

微重力条件下气／液两相流压降实验研究

利用俄罗斯IL-76失重飞机对微重力条件下方形截面管道内的水/气两相流压降进行了实验研究．利用本次微重力气／液两相流压降实验结果对一些目前常用的基于地面常重力实验结果的两相流压降预测模型(均相模型、Lockhart-Martinelli—Chisholm模型和Friedel模型)在微重力条件下的适用性进行了评估．比较发现,均相模型和Lockhart-Martinelli—Chisholm模型预测结果和实验数据差异很大;而Friedel模型的预测结果尽管也与实验数据有着明显的差别,但在这些模型中是误差最小的．因此,可以利用Friedel模型来对微重力气／液两相流压降进行初步预估,而新的、更精确的模型将依赖于更有物理意义的分析方法及不同重力条件下气／液两相流压降实验的结果．     

一个非线性电路模型的混沌态特性分析

对非线性系统进行分析时,可能出现测量结果为混沌态,以一个非线性电路模型为例。分析混沌态的几率密度并提出“赝温度”的新概念,以表征混沌态的赝随机变化的剧烈程度。从而为非线性系统的混沌态进行“乱中有序”的定量描述提供了一种新方法。     

Axisymmetric fundamental solutions for a finite layer with impeded boundaries

Axisymmetrie fundamental solutions that are applied in the consolidation calculations of a finite clay layer with impeded boundaries were derived. Laplace and Hankel integral transforms were utilized with respect to time and radial coordinates, respectively in the analysis. The derivation of fundamental solutions considers two boundary-value problems involving unit point loading and ring loading in the vertical. The solut-ions are extended to circular distributed and strip distributed normal load. The computation and analysis of set-tlements, vertical total stress and excess pore pressure in the consolidation layer subject to circular loading are presented.     

实时数据通讯的方法及应用

介绍了在Windows98环境下,通过Modem和公用电话网(PSTN),利用设置好的"拨号网络",实现PC机系统之间的实时数据传输的方法。讨论了运用VB语言,调用WindowsAPI所提供的远程访问函数,设计了一个专用于"拨号网络"通讯的类,并详细介绍了该类的功能和用法。     

Advances and applications of electrohydrodynamics

Electrohydrodynamics (EHD) has been applied in many areas, such as EHD enhanced heat transfer, EHD pump, electrospray mass spectrometry, electrospray nanotechnology, etc. The basic theories underlying these applications and a brief review of the EHD advances are presented in this paper. As electrospray is an important branch of EHD,the applications of electrospray are particularly discussed.Finally, this paper gives some suggestions for the future research and development.     

基于GA优化的城市可持续发展评价的普适公式

在分析城市可持续发展指标体系的特征基础上,分别确定了用遗传算法优化的持续、协调、结构和集聚４类单项指标的发展指数的普适公式;并对可持续发展的不同层次指标分别采用广义对比分析法和层次分析法进行加权,建立了城市可持续发展的综合指数评价模型。模型应用于乌鲁木齐的可持续发展评价结果与实况定性分析结果一致。该评价模型简单、实用,具有可比性和通用性。