Methylation of hippocampal phosphatidylethanolamine and proteins during long-lasting potentiation

Whereas the monomethylation of hippocampal phosphatidylethanolamine is decreased following the induction of long-lasting potentiation of the CA1 population spike, carboxymethylation of proteins is unaffected.     

Evidence for ecology's role in speciation

A principal challenge in testing the role of natural selection in speciation is to connect the build-up of reproductive isolation between populations to divergence of ecologically important traits. Demonstrations of 'parallel speciation', or assortative mating by selective environment, link ecology and isolation, but the phenotypic traits mediating isolation have not been confirmed. Here we show that the parallel build-up of mating incompatibilities between stickleback populations can be largely accounted for by assortative mating based on one trait, body size, which evolves predictably according to environment. In addition to documenting the influence of body size on reproductive isolation for stickleback populations spread across the Northern Hemisphere, we have confirmed its importance through a new experimental manipulation. Together, these results suggest that speciation may arise largely as a by-product of ecological differences and divergent selection on a small number of phenotypic traits.     

Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia

The duplication of entire genomes has long been recognized as having great potential for evolutionary novelties, but the mechanisms underlying their resolution through gene loss are poorly understood. Here we show that in the unicellular eukaryote Paramecium tetraurelia, a ciliate, most of the nearly 40,000 genes arose through at least three successive whole-genome duplications. Phylogenetic analysis indicates that the most recent duplication coincides with an explosion of speciation events that gave rise to the P. aurelia complex of 15 sibling species. We observed that gene loss occurs over a long timescale, not as an initial massive event. Genes from the same metabolic pathway or protein complex have common patterns of gene loss, and highly expressed genes are over-retained after all duplications. The conclusion of this analysis is that many genes are maintained after whole-genome duplication not because of functional innovation but because of gene dosage constraints.     

Ultra-high suppression microwave photonic bandstop filters

A bandstop radio frequency （RF） filter can enhance the capabilities of RF communication systems by removing unwanted signal components such as distortion, or interference. In modem cognitive radio systems, frequency agile filters are desired to block interferers with dynamically changing frequencies and powers. These filters should be tunable over a broad frequency range, and they should be capable of suppressing very strong signals with a high resolution. To simultaneously meet these requirements with traditional RF filters are highly chal- lenging. Microwave photonic （MWP）, a technology that primary deals with the generation, distribution, and processing of high speed RF signals using photonic techniques and components, is promising for creating frequency agile RF filters. However, traditional MWP filters are lacking the high resolution and peak suppression exhibited by state-of-the-art RF electrical filters. We recently introduced a new class of MWP notch filter which is free from this limitation. This scheme allowed the creation of anomalously high suppression MWP notch filter from virtually any kind of optical resonance, irrespective of its type （gain or absorp tion）, or its magnitude. This enabled, for the first time, simultaneous optimization of the MWP filter resolution, peak attenuation, and frequency tuning range. In this paper, we present the analysis of notch filter response creation using the novel sideband processing technique. We then show the applicability of this technique to a wide range of optical filters. We compare simulated and experimental results of the notch filter response created using three types of optical filter commonly used in MWP signal processing, namely stimulated Brillouin scattering （SBS）, an integrated optical ring resonator （ORR）, and a phase-shifted fiber Bragg-grating （FBG）.     

p53 mutant mice that display early ageing-associated phenotypes.

The p53 tumour suppressor is activated by numerous stressors to induce apoptosis, cell cycle arrest, or senescence. To study the biological effects of altered p53 function, we generated mice with a deletion mutation in the first six exons of the p53 gene that express a truncated RNA capable of encoding a carboxy-terminal p53 fragment. This mutation confers phenotypes consistent with activated p53 rather than inactivated p53. Mutant (p53+/m) mice exhibit enhanced resistance to spontaneous tumours compared with wild-type (p53+/+) littermates. As p53+/m mice age, they display an early onset of phenotypes associated with ageing. These include reduced longevity, osteoporosis, generalized organ atrophy and a diminished stress tolerance. A second line of transgenic mice containing a temperature-sensitive mutant allele of p53 also exhibits early ageing phenotypes. These data suggest that p53 has a role in regulating organismal ageing.     

Drought sensitivity shapes species distribution patterns in tropical forests

Although patterns of tree species distributions along environmental gradients have been amply documented in tropical forests, mechanisms causing these patterns are seldom known. Efforts to evaluate proposed mechanisms have been hampered by a lack of comparative data on species' reactions to relevant axes of environmental variation. Here we show that differential drought sensitivity shapes plant distributions in tropical forests at both regional and local scales. Our analyses are based on experimental field assessments of drought sensitivity of 48 species of trees and shrubs, and on their local and regional distributions within a network of 122 inventory sites spanning a rainfall gradient across the Isthmus of Panama. Our results suggest that niche differentiation with respect to soil water availability is a direct determinant of both local- and regional-scale distributions of tropical trees. Changes in soil moisture availability caused by global climate change and forest fragmentation are therefore likely to alter tropical species distributions, community composition and diversity.     

DNA sequence and analysis of human chromosome 8

The International Human Genome Sequencing Consortium (IHGSC) recently completed a sequence of the human genome. As part of this project, we have focused on chromosome 8. Although some chromosomes exhibit extreme characteristics in terms of length, gene content, repeat content and fraction segmentally duplicated, chromosome 8 is distinctly typical in character, being very close to the genome median in each of these aspects. This work describes a finished sequence and gene catalogue for the chromosome, which represents just over 5% of the euchromatic human genome. A unique feature of the chromosome is a vast region of approximately 15 megabases on distal 8p that appears to have a strikingly high mutation rate, which has accelerated in the hominids relative to other sequenced mammals. This fast-evolving region contains a number of genes related to innate immunity and the nervous system, including loci that appear to be under positive selection--these include the major defensin (DEF) gene cluster and MCPH1, a gene that may have contributed to the evolution of expanded brain size in the great apes. The data from chromosome 8 should allow a better understanding of both normal and disease biology and genome evolution.