江西龙宫洞不同季节土壤微生物群落结构比较

采用非培养法和传统培养法相结合,利用Illumina高通量测序技术对江西省九江市彭泽县龙宫洞微生物的16S rDNA和ITS相关区域进行测序及生物信息学分析,通过实时荧光定量PCR(qPCR)对洞穴土壤中细菌和真菌含量进行绝对定量,探究不同季节对洞穴土壤微生物群落结构的影响,同时观察相应可培养微生物分布情况.结果表明:...     

Temporal and spatial distribution of tropospheric CO<Subscript>2</Subscript> over China based on satellite observations

Mid-troposphere CO₂ data retrieved by the AIRS (atmospheric infrared sounder) were validated with five ground-based stations and aircraft measurements in the Northern Hemisphere. AIRS CO₂ products show good agreement with ground and aircraft observations. The data had a monthly average accuracy better than 3 ppmv. In this study, the spatial and temporal distribution of mid-troposphere CO₂ from January 2003 to December 2008 was analyzed based on this satellite product. The average concentration of atmospheric CO₂ was higher in the Northern Hemisphere than in the Southern Hemisphere. The yearly average results show a gradual increase from 2003 to 2008. In China, the annual growth rate was about 2 ppmv/a, similar to the United States, Europe, Australia and India, but was slightly lower than Canada and Russia. Mid-troposphere CO₂ concentrations were higher over northern China than over southern areas, due to differences in natural conditions and industrial layout. There were four centers of high CO₂ concentration between 35° and 45°N over China, with low concentrations over Yunnan Province. There was a significant seasonal CO₂ variation with peak concentration in spring and the lowest concentration in autumn.     

疏水蛋白rHGFI与rHFBI的发泡能力研究

将巴斯德毕赤酵母异源表达出的疏水蛋白rHGFI和rHFBI修饰硅化玻璃后进行玻璃表面接触角测定.结果显示:蛋白处理的硅化玻璃表面由疏水变为亲水.蛋白溶液表面张力测定结果表明:rHGFI和rHFBI是表面活性非常强的蛋白,均可使表面张力降低.以常用的食品乳化剂酪蛋白酸钠为对照,检测rHGFI和rHFBI蛋白的发泡能力和泡沫稳定性.0.1%的rHGFI和rHFBI蛋白溶液发泡能力明显高于同浓度的酪蛋白酸钠溶液,rHFBI蛋白溶液的泡沫稳定性最佳.     

高职院校大学生职业指导目标达成的途径

该文对开展高职院校大学生职业指导工作的目标及途径进行探讨.目前大学生就业形势严峻,高职院校职业指导工作,开展水平、情况也不容乐观.文章从一个高职院校职业指导老师的角度,结合开展职业指导实际工作情况,对高职院校职业指导工作目标和途径进行了积极的思考和探讨.     

我看《牛拖船》

在散文集<牛拖船>中,作者运用朴实的语言、独特的视角、纯真的情感,集中地描绘了萧山沙地的风情,赋予了作品现实主义的精神.这种现实性的获得是由沙地的历史和自然环境、丰富的生活民情、作者自身的经历所决定的.     

高等学校生态道德教育方法探析

生态危机是人类对自然态度的非合理性投射,实质上是自我价值危机.它不仅需要科学技术来解决,而且包含着强烈的道德诉求.以生态道德理念唤醒麻木的心灵,去除价值巫魅,追求诗意生存,教育将承受之重!高等学校生态道德教育尚付阙如,大学生生态道德缺失的现实都昭示了加强高校生态道德教育刻不容缓.生态道德教育的方法主要有:道德讨论法,研究性学习,体验教育等.     

A combination of leaf rust resistance gene Lr34 and lesion mimic gene lm significantly enhances adult plant resistance to Puccinia triticina in wheat

Leaf rust caused by Puccinia triticina is an economically-important disease in wheat worldwide.A combination of different types of resistance genes may significantly enhance rust resistance under rust-favorable conditions.To investigate the interactions between the rust resistance gene Lr34 and the lesion mimic gene lm on 1BL in Ning 7840,a segregating F8-10 population of 180 recombinant inbred lines was developed from Ning 7840/Chokwang and evaluated for both lesion mimic expression and leaf rust response at the adult plant stage in a greenhouse.A major quantitative trait locus(QTL),derived from Sumai 3,was co-localized with Lr34 on chromosome 7D and explained 41.5% of phenotypic variations for rust severity and 22.1% for leaf tip necrosis(LTN).The presence of Lr34 was confirmed by Lr34-specific markers cssfr1 and cssfr2 in Ning 7840 and Sumai 3.Unlike Lr34,lm conditioned a spontaneous lesion mimic phenotype and had a significant effect on reducing uredinial size,and a smaller effect on severity.Additive effects were observed between lm and Lr34 for severity and LTN,and an epistatic effect was observed for infection type.Single marker analysis also identified several other QTL with minor effects on severity,infection type,or LTN.     

浅谈事业单位会计制度改革

随着我国财政体制改革与事业单位改革的不断深化,事业单位会计核算内外环境已发生巨大变化,越来越不适应形势发展的需要,这就使得现行事业单位会计制度改革势在必行.本文在阐述事业单位改革以及会计制度改革必要性的基础上,指出事业单位会计制度存在的主要问题,最后提出对事业单位会计制度改革的几点建议.     

浅谈钻井冲洗液在钻探施工中的重要性

介绍了常用钻井冲洗液的类型及适用地层，通过亲自实践总结出钻井冲洗液在钻探施工中的重要性。为今后相关钻探施工工作提供借鉴。