Immobilization and Hybridization of cDNA Arrays on the Glass Surface

IntroductionMicroarray technology uses microscopic arrays ofDNA molecules immobilized on solid supports forbiomedical analysis,such as gene expressionanalysis,polymorphism and mutation detection,drug screening,and gene discovery[1 3] .　 Several approaches can be used to preparemicroarrays. DNA can be synthesized in situ onglass using combinatorial chemistry approach.This method typically produces microarraysconsisting of groups of oligonucleotides ranging insize from 1 0 to 2 5 bases wherea…     

Gene Disease Diagnostic System

IntroductionBinary optics[1,2 ] is a new technology which uses amicro- ion- etching film layer on a thin glass plateinstead of a common cubic glass lens.With binaryoptics,optical elements (such as lens andgratings) can be fabricated as integrated circuit(IC) devices.The binary optics can be integratedinto a laboratory on- a- chip[37] ,which includes thethree classic steps,sample preparation,biochemical reaction,and detection analysis.These small biochips have been used forpolymerase chain rea…     

Point Mutation Identification Using On-Chip Ligase Detection Reaction

An efficient method was developed to detect point mutations using oligonucleotide microarrays and the ligase detection reaction (LDR). Allele-specific LDR primers were immobilized on polylysine-coated glass slides to perform LDR on a chip. The spotting concentration and detection limit were analyzed using a synthesized oligonucleotide as a template. The optimal primer spotting concentration was 20 μmol/L and the lowest detectable template concentration was 0.33 nmol/L. The method was successfully employed to identify malignant mutations of hypertrophic cardiomyopathy. Asymmetric polymerase chain reaction was employed to prepare single stranded DNA as LDR templates from cloned plasmids. The discrimination ratios for AC, TC, GT, TT, GA, and AA mismatches are 32.82, 44.24, 17.75, 18.34, 11.66, and 8.91, respectively. This method may allow construction of multiple mutation detection systems.     

智能IC卡电度表管理信息系统

文章介绍了智能ＩＣ卡电度表管理信息系统的功能及工作原理,并阐述了该系统的关键部分：由单片机应用系统组成的ＩＣ卡电度表的设计。     

走出研究面向大众的生物芯片

生物芯片技术是应现代分子生物学研究的重大需求,在学科交叉不断深入的基础上诞生的,现已成为国际上的热点研究领域.     

生物芯片技术的发展与应用

生物芯片技术是９０年代初期发展起来的一门新兴技术,通过微技术制作的生物芯片,可以民千上万乃至几恨个生命信息集成在一个很小的芯片上,达到对基因、怕和 细胞等进行分析和检测的目的,用这些生物芯片制作的各种生化分析仪和传统仪器相比较具有体积小、重量轻、便于携带、无污染、分析过程自动化、分析速度快、所需样 度剂少等诸多优点,这类仪器的出现将给生命科学研究、疾病诊断、新药开发、生物武器战争、司法鉴定、食品     

一种非公平条件下的多SCP智能网过载控制算法

智能业务的螺旋形膨胀不但使智能网的结构向着多SCP的方向发展,更使得智能网SCP过载控制机制显得极其重要.首先给出了一套不依赖集中控制的完全分布式多SCP智能网组网方案,然后给出了在这种网络环境下的呼叫分发算法以及在非公平条件下的基于ACG算法的SCP过载控制算法.