单倍体小麦与簇毛麦的不对称体细胞杂交

以小麦杂２４２（Ｔｒｉｔｉｃｕｍａｅｓｔｉｖｕｍ,ｃｖ．２４２）花药来源的愈伤组织分离原生质体作受体;簇毛麦（Ｈａｙｎａｌｄｉａｖｉｌｏｓａ）幼胚来源的愈伤组织原生质体经２２０μＷ／ｃｍ２紫外线照射３０ｓ作供体,用ＰＥＧ法诱导融合,最后获得再生愈伤组织．对融合再生的４个最先长大的细胞系进行染色体,同工酶和ＲＡＰＤ分析．结果表明,这些细胞系均为体细胞杂种,ＲＡＰＤ技术可作为小麦体细胞杂种早期鉴定的有效方法     

中国大陆东部盆地构造动力学分析

在中国大陆东部地区,松辽、渤海湾和苏北等—系列中、新生代大中型含油气盆地与郯庐断裂具有密切的时空关系。郯庐断裂活动是邻近地区断裂变形、深部物质作用、岩石圈减薄、岩浆作用以及盆地形成和演化的主要控制因素。断裂活动和盆地动力学过程可以分为３个阶段。①在印支至早燕山运动期间,由于太平洋板块向北西俯冲,郯庐断裂发生大规模左行压扭活动。在松辽地区,派生的应力场使早期的地壳断裂成为郯庐断裂的分支,并发生张剪变形;在渤海湾和苏北地区,早期的两组剪切断裂联合成北凸的弧形断裂。由于断裂的触发与减压作用,地壳上部进一步张裂,形成断陷盆地。②从晚侏罗世开始至早白垩世,太平洋板块的俯冲作用时强时弱,郯庐断裂压扭与拉张裂陷交替进行。同时,由于重力均衡和深部幔隆的收缩作用,使松辽盆地整体下沉,渤海湾和苏北地区仍是幔隆和断陷盆地形成时期。③新生代期间,郯庐断裂分段活动,由于印度板块与欧亚板块的强烈碰撞效应,渤海湾、苏北地区和郯庐断裂带南段共同受到北西—南东方向的伸展变形,地幔再次拱升,渤海湾和苏北盆地形成     

Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract

When added with cytochrome c, Xenopus laevis egg extract XS-150 can induce exogenous nuclei undergoing apoptosis. Apoptosis specific DNase XAD was activated during this process, and cut chromatin between nucleosome, leading to DNA Ladder in electrophoresis. Our results showed that an inhibitor of XAD, IXAD, exists abundantly in normal egg extract, its molecular weight is about 40 ku. Normally, IXAD exists either in the form of dimmer or in complex with XAD. It was degraded during apoptosis, releasing active XAD. The results of Western assay and cross-inhibition showed that IXAD was likely homologous to DFF45 in structure and function. At the same time, these results also indicated that the pathway in apoptosis was conserved in evolution.     

A study of the microstructure of CTAB/1-butanol/octane/ water system by PGSE-NMR and Cryo-TEM

In this work, the effect of octane concentration on the phase behavior of CTAB/water/1-butanol system was studied by using pulsed field gradient spin-echo NMR measurements and freeze fracture electron microscopy (Cryo- TEM and FFEM). When the octane concentration increases, the liquid crystalline phase is destabilized and a continuous single-phase microemulsion region from the water apex to the oil apex is formed. The conductivity behavior has a distinct percolative phenomenon, which indicates that the single-phase microemulsion is changed continuously from oil- in-water (o/w) structure via a bicontinuous structure to water-in-oil (w/o) structure. This result is consistent with those of the PGSE-NMR, Cryo-TEM, and FFEM. In the w/o region, the self-diffusion coefficient of water is relatively high ((1—6)×10^-10 m·s^-1) due to the higher solubility of water in the continuous phase consisting of octane (10% by weight) and 1-butanol. The penetration of a large amount of octane molecules between surfactant chains results in the much lower self-diffusion coefficient of octane.     

Investigating the function of Akt by tet-off inducible expression system

A tet-off inducible cell line named BBT derived from BA/F3b cell line was constructed and the effect of this inducible expression system was significant when detected by tet-off responded luciferase reporter gene assay. Then tet-off responded Akt expression plasmid was transfected into BBT cells, and the stable cell lines were screened. The result of Northern blot showed that the expression of akt was significantly inducible. The clone with the best inducible effect was selected and named BBA for investigating the function of Akt. We found that Akt could significantly inhibit zinc-induced decrease of cell viability when assayed by MTT method. And the flow cytometric analysis showed that Akt could markedly repress zinc-induced apoptosis.     

论精神价值的特性及其在人类生活中的地位和作用

精神价值具有五个方面的基本特性 :满足人的精神需要 ;其价值量不能用货币形态或市场价格来衡量 ,而是根据其对人类生活近期和长期的作用力和影响力来衡量 ;具有无形的特征 ,它作用于使用者但又不被使用者消耗掉 ;具有无限的传播性、放大性和增值性 ;具有长久性和永久性。因而 ,精神价值在人类生活中的地位和作用首先表现在精神价值并不是人类生活的奢侈品 ,而是人类生活的必须品。其次 ,精神价值是人类创造经济价值的动力和源泉。第三 ,精神财富是人类文明的基石和支柱     

喀斯特洞穴大型底栖动物群落结构与环境因子的关系——以贵州惠水3个洞穴为例

为了解喀斯特洞穴大型底栖动物群落结构及其与环境因子的关系,选择贵州省惠水县的龙洞、牛洞、龙潭洞作为研究对象,分析洞穴大型底栖动物的群落组成、现存量、生物多样性特征,并且运用Spearman相关性分析洞穴部分环境因子与底栖动物群落结构的关系,同时基于底栖动物生物学指数对洞穴水质作出评价。结果表明：夏、冬两季共采集到大型底栖动物39种,隶属4门5纲10目31科,以节肢动物和软体动物为主。洞穴大型底栖动物密度在季节上表现为夏季>冬季,在空间上表现为有光带>弱光带>黑暗带,生物量则无明显时空变化。Spearman相关性分析表明,夏季影响洞穴大型底栖动物群落结构的主要环境因子是光照强度、沉积物有机质和浊度,冬季影响洞穴大型底栖动物群落结构的主要环境因子是光照强度、总氮和水温。Shannon-Wiener指数、Margalef指数及科级水平生物指数(Family-Level Biotic Index, FBI) 3种生物指数评价洞穴水质状况有差异,FBI的评价结果与洞穴的实际水质情况更符合。     

飞行体物质的自隐形特征

应用运动体的相对论变换诱导出其自身的介电常数和磁导率变化，开拓出一类新型的运动体自隐形机制首先明确自隐形概念，再介绍隐形原理和自隐形关系最后指出这些结果可供隐形功能材料设计参考．     

新型磺酰胺类除草剂的设计、合成和生物活性

利用已构建的ALS抑制剂的药效团模型，设计并合成了四种新型磺酰胺类化合物，并进行了结构表征，利用平皿法和ALS酶的离体测定法进行了生物活性测定，结果表明它们具有一定的生物活性，为进一步开发具有实用价值的除草剂作了有益的尝试。     

有序性原则与学生自学能力的培养

本文以普物教学为例探讨了遵循有序性原则,运用“五环式”教学方法培养理工科学生的自学能力的问题.