Electrostatic effect on electron transfer between cytochrome b5 and cytochrome c

The binding and electron transfer between wild type, E44A, E56A, E44/56A, E44/48/56A/D60Aand F35Y variants of cytochrome b5 and cytochrome c were studied. When mixed with cytochrome c, the cytochrome b5E44/48/56A/D60A did not show the typical UV-vis difference spectrum of absorption, indicating that the alteration ofthe surface electrostatic potential obviously influenced the spectrum. The electron transfer rates of wild type cytochromeb5, its variants and cytochrome e at different temperature and ionic strength exhibited an order of F35Y > wild type >E56A > E44A > E44/48/56A/D60A. The enthalpy and entropy of the reaction did not change obviously, suggestingthat the mutation did not significantly disturb the electron transfer conformation. The investigation of electron transfer rateconstants at different ionic strength demonstrated that electrostatic interaction obviously affected the electron transfer pro-cess. The significant difference of Cyt b5 F35Y and E44/48/56A/D60A from the wild type protein further confirmed thegreat importance of the electrostatic interaction in the protein electron transfer.     

国家高新区研发投入影响因素研究

我国高新区研发投入水平偏低,在建设创新型国家及提升我国自主创新能力方面的作用没有得到充分发挥。对国家高新区研发投入强度的影响因素进行了回归分析,发现市场竞争程度、企业利润水平、企业规模对高新区研发投入有正向影响,而出口规模、资产负债率则对高新区研发投入有负面影响,据此提出了提高高新区研发投入水平的政策建议。     

电火工品的感度控制和非破坏预测

介绍了电火工品用瞬态脉冲试验控制感度的基本方法，讨论了电火工品发火感度无损检测的发展前景，认为发展单发性能预测和相应的仪器有很大的可能性。     

Speed-Sensorless Control Using Elman Neural Network

1. INTanDUCTIONControlling induction motor without sensor is available nowadays. In order to aclileve two-level closed lOops,i. e., digital current lOop and digital speed loop, it is necessary to sense.and digitize the motor phase currentand motor speed. Howver, the sensors involved have the followillg disadvantages. First, they aJre eXPensiveand make up a significan portion of the total cost. Second, they may result in electrical or mechaulcal fault.Based on speed estimation, it is pos…     

21世纪高师地理信息系统教学刍议

针对地理信息系统课程的特点，对高等院校２１世纪地理信息系统课程的教学内容和教学方法作了探讨。     

言近义远 妙趣横生——试析比喻修辞格在英谚中的运用及其语义效果

本文从美学修辞入手 ,借助一些具代表性的例子探讨了英语谚语中比喻修辞格的运用及其产生的语义效果。以期对更好地欣赏比喻及其它修辞手段所赋予英语谚语的美学魅力有所俾益和帮助     

α-硫辛酸-(8-生物素酰胺基-3,6-二氧辛基)酰胺的合成

选择α-硫辛酸、长醚链二胺、生物素为主要原料,合成了α-硫辛酸-(8-生物素酰胺基-3,6-二氧辛基)酰胺;并用1HNMR、GC-MS、IR等对中间体及目标产物的分子结构进行了表征.通过改变生物素分子中的侧链部分,可合成不同种类的生物素衍生物,增加了生物素-亲合素/链霉亲合素/中性亲合素自组装膜体系的材料的种类和应用.     

瞬态电晕放电成像的实验研究

阐述了瞬态电晕放电成像的物理过程，根据气体放电的流光理论，采用纳秒脉冲放电技术，获得清晰的电晕放电的硬币成像图像，并对脉冲峰值电压及相对湿度等成像条件进行了探讨。     

反射法检测桩基质量的几个影响因素

结合工程实例,对于影响反射法检测桩基质量的几个因素作了客观的分析,并且提出了初步的改进意见,对桩基检测有一定的指导意义.     

关于三次参数曲线的形状控制

讨论了平面三次参数曲线在端点出现拐点，二重点，尖点的情形，同时，讨论了曲线端点处的切向量平行等特殊情形。