利用有机溶剂法快速纯化绿色荧光蛋白

介绍非色谱GFP纯化方法——有机溶剂纯化法．该方法在短时间内（30min）即可获得大量较高纯度（91．7％）,且荧光光谱特征不改变的GFP蛋白．纯化过程简单,无需昂贵的仪器和设备,适合绝大多数中小实验室使用．     

Effect of Dy substitution on the microstructure and magnetic properties of nanograin Nd-Fe-B single-phase alloys

Nanocrystalline single-phase alloys with the nominal compositions (at%) of Nd_12.3-xDy_xFe_79.7Zr_0.8Nb_0.8Cu_0.4B_6.0 (x=0, 0.5, 1.5, and 2.5) were prepared by melt-spinning and subsequent annealing. X-ray diffraction analysis shows that the as-spun ribbons were mainly composed of the amorphous phase. A slight content of Dy stabilizes the amorphous phase during annealing treatment. The grain size becomes smaller and the coercivity of the annealed ribbon is gradually improved with the increase of Dy content. Excessive Dy is harmful to the remanence. It is found that no intergranular phase exists between the grains by high-resolution transmission electron microscopy, and the grain boundaries are crystallographically coherent in the optimally annealed sample. The optimum magnetic properties of remanence (J_r=1.09 T), coercivity (H_ci=1048 kA/m), and maximum magnetic energy product ((BH)_m=169.5 kJ/m3) are obtained from the x=0.5 ribbon in a post heat-treated state (700℃, 10 min).     

Multi-pollutants simultaneous removal from flue gas

The multi-stage humidifier semi-dry flue gas cleaning technology, the CRS plasma flue gas cleaning technology and oxidative additive flue gas cleaning technology were investigated for multi-pollutants removal. The semi-dry flue gas cleaning technology using multistage humidifier and additive can improve oxidation and absorption, and it can achieve high multi-pollutants removal efficiency. The CRS discharge can produce many OH radicals that promote NO oxidation. Combining NaOH absorption can achieve high deSO2 and deNOx efficiencies. It is very fit of the reconstruction of primary wet flue gas desulfurization (WFGD). In addition, Using NaClO2 as additive in the absorbent of WFGD can obtain very high removal efficiency of SO2 and NOx.     

钢筋混凝土梁高温试验现象及损伤分析

本文进行了相同条件下不同受火时间的钢筋混凝土梁的高温试验,通过试验数据及表观现象分析,研究火灾中受火时间分别为0小时,0.5 小时,1小时,1.5小时,2小时,3小时,钢筋混凝土梁构件宏观方面的变化,包括质量损失及表观颜色,裂缝,保护层等的变化规律和高温前后纵波波速的变化规律,总结出不同受火时间对钢筋混凝土梁构件损伤程度的影响。     

强力砂带平面磨削温度分布的有限元分析及试验研究

本文分析了国内外磨削温度的计算方法，指出了用有限元法及有限差分法在时间递推上的优势，用以处理二维不稳态磨削温度场，得出了有重要理论和实用价值的磨削温度计算公式，拟定了相应的计算机程序，以不同磨削用量下的温度场进行了数值计算及大量的试验对比，采用了Ｐｅｋｌｅｎｉｋ测温法通过计算机实现数据采集，试验结果表明，理论计算值与试验值相当吻合，并得出一些重要结论。     

An ERP study on shift of spatial attention resulting from number processing

Using the “stimulus-delay-target” experimental model, the brain mechanism of visual-spatial attention shift after number processing was conducted on 13 participants by using event-related potentials (ERP) technology. Results show that influence of the number processing on the spatial attention is indexed by the P3 component; parietal lobes at both sides may be the main neural substrate for number processing and spatial attention, and the numerical-spatial interaction mainly occurs in the response selection stage.     

8个经典4色Ramsey数的新下界

提出了计算经典多色Ｒａｍｓｅｙ数Ｒ（ｑ１,ｑ２,…,ｑｎ）下界的一个算法,得到８个新的下界：Ｒ（３,３,３１１）≥３１４,Ｒ（３,３,３,１２）≥３５４,Ｒ（３,３,３,１３）≥４３２,Ｒ（３,３,３,１４）≥４６２,Ｒ（３,３,３,１５）≥,５２２,Ｒ（３,３,３,１６）≥６１８,Ｒ（３,３,３,１７）≥６７４,Ｒ（３,３,３,１８）≥７７０。     

用神经网络理论进行纤维水泥砼的配合比设计

根据公路桥梁桥面铺装对纤维水泥砼的使用性能要求，将一批试验数据作为样本，通过神经网络对其进行学习和训练，得到了相应的神经网络权值矩阵和阈值向量。计算结果与实际试验结果的对比表明，采取本方法可以提高纤维水泥砼配合比的设计效率。     

散热器进流系数算法的研究

详细剖析了跨越式单管采暖系统中，水流过散热器的温降对进流系数的影响，并针对现在所采用的计算方法的不合理之处，建立了一种新的数学模型来计算散热器的进流系数。