Quantal transmitter secretion from myocytes loaded with acetylcholine.

It is well known that transmitter secretion requires specialized secretory organelles, the synaptic vesicles, for the packaging, storage and exocytotic release of the transmitter. Here we report that when acetylcholine (ACh) is loaded into an isolated Xenopus myocyte, there is spontaneous quantal release of ACh from the myocyte which results in activation of its own surface ACh channels and the appearance of membrane currents resembling miniature endplate currents. This myocyte secretion probably reflects Ca(2+)-regulated exocytosis of ACh-filled cytoplasmic compartments. Furthermore, step depolarization of the myocyte membrane triggers evoked ACh release from the myocyte with a weak excitation-secretion coupling. These findings suggest that quantal transmitter secretion does not require secretory pathways unique to neurons and that the essence of presynaptic differentiation may reside in the provision of transmitter supply and modification of the preexisting secretion pathway.     

一种基于数字水印的错误检测和定位技术

为了增强视频通信系统在有误码信道的适应能力 ,提出了一种基于数字水印技术的错误检测方法 ,使得解码器可以对误码块进行有效的补偿。其原理为编码器在量化后离散余弦变换 (DCT)系数中嵌入易碎的数字水印 ,解码器可以通过提取水印从而确认错误以及错误发生的首宏块位置。仿真结果证明 :在信道误码率为 5× 10 - 4时 ,与常用的基于语法的方法相比 ,本方法的错误检测率是其的 1.32～ 1.82倍 ,错误正确定位率是其的 2 .95～ 5 .32倍 ,而且 PSNR只有微弱降低 ,同时算法复杂度很低     

新型全固态铁离子敏感电极研究

采用稀土电极材料六硼化镧制备了一种新的全固态铁离子敏感电极。研究了该敏感电极在常见介质中对Ｆｅ３＋的响应行为。结果表明，该铁离子敏感电极在１．５ｍｏｌ／ＬＨ３ＰＯ４介质中的线性响应范围、检测下限和响应斜率分别为１．０×１０－５～１．０×１０－１ｍｏｌ／ＬＦｅ３＋，６．０×１０－６ｍｏｌ／ＬＦｅ３＋和６０ｍＶ／ｐＣ，且具有较好的选择性。     

A calcium sensor in the sodium channel modulates cardiac excitability.

Sodium channels are principal molecular determinants responsible for myocardial conduction and maintenance of the cardiac rhythm. Calcium ions (Ca2+) have a fundamental role in the coupling of cardiac myocyte excitation and contraction, yet mechanisms whereby intracellular Ca2+ may directly modulate Na channel function have yet to be identified. Here we show that calmodulin (CaM), a ubiquitous Ca2+-sensing protein, binds to the carboxy-terminal 'IQ' domain of the human cardiac Na channel (hH1) in a Ca2+-dependent manner. This binding interaction significantly enhances slow inactivation-a channel-gating process linked to life-threatening idiopathic ventricular arrhythmias. Mutations targeted to the IQ domain disrupted CaM binding and eliminated Ca2+/CaM-dependent slow inactivation, whereas the gating effects of Ca2+/CaM were restored by intracellular application of a peptide modelled after the IQ domain. A naturally occurring mutation (A1924T) in the IQ domain altered hH1 function in a manner characteristic of the Brugada arrhythmia syndrome, but at the same time inhibited slow inactivation induced by Ca2+/CaM, yielding a clinically benign (arrhythmia free) phenotype.     

生存教育:素质教育的重要内容

“生存教育”是以培养和训练学生生存能力为主要目的的教育。形势发展的需要和现实的差距，要求我们必须把生存教育当作素质教育的重要任务和内容来抓。生存教育要使学生学会在“独自”情况下生存，学会在压力下生存，学会在紧急状况下生存，学会在集体中生存，学会在逆境中生存。应从多个方面、多种途径实施生存教育。     

Erosion and Sediment Production in Small Watershed in Purple Hilly Areas and Prevention Techniques

Purple Soil distributes extensively and mainly in China. Because of abundant easily weatherable parent rocks/materials and unstable soil structure, and also influenced by parent materials, usage systems, and slope gradients, erosion and sediment production of purple soils are very severe with main fashions of water erosion and gravitational erosion. Basing on observed data in small watersheds, rainfall erosivity,vegetation coverage, previous soil water content, flow and relating factors such as climate, topograph of small watershed,land usage, and soil kinds are all the influence factors of erosion and erodibility of purple soil as well as sediment production and transport in small watershed of purple hilly areas.The effective technological countermeasures of ecosystem restoration, agricultural tillage for water conservation and erosion prevention, agriculture project, and soil changing for fertility and anti-erosion were provided.     

三邻菲咯啉合亚钌-亚硫酸根-氯酸钾体系化学发光法测定葡萄酒中亚硫酸盐总量

提出了Ru(phen) 32 +-SO32 - -KClO3(phen =1 ,1 0菲咯啉 )体系化学发光法测定溶液中亚硫酸盐含量的方法 .SO32 - 浓度与化学发光强度在 1 .0× 1 0 - 6～ 1 .0× 1 0 - 3 mol/L范围内成正比 ,检出限为 2 .32× 1 0 - 6mol/L (S/N =3) ,1 .0× 1 0 - 4 mol/LSO32 - 溶液重复 6次测定结果的相对标准偏差为 3.8% .该方法成功地用于白葡萄酒中的亚硫酸盐总含量的测定 ,结果令人满意 .     

移民村落的权力博弈与移民的适应——以广东三峡移民村落白村为例

通过对一个安置在广东的三峡移民村落白村首任村长上任、下台的调查与分析,探究了移民村落的乡村权力“博弈”过程,以及这种乡村权力与移民适应的关系,指出帮助移民获得适当的社会资本是帮助移民更好地适应的策略之一。     

Graves甲亢合并周期性麻痹的131I疗效分析

目的了解Graves甲亢合并周期性麻痹的发病机制及131I对其治疗价值.方法对34例Graves甲亢合并周期性麻痹的患者均采用131I治疗.结果34例患者经予131I治疗后,其中有8例治疗无效于6月后进行了2次131I治疗.6个月治愈率76.47%%(26/34),12个月治愈率97.06%(33/34),总的有效率为100%;其中早发甲低者3例,发生率8.82%,予替代治疗后均获好转;晚发甲低者4例,发生率11.76%.结论131I对Graves甲亢合并周期性麻痹的疗效肯定,使用安全,疗程短,应考虑作为首选治疗.