宁乡青铜文化起源再探索

宁乡青铜文化是在中原青铜文化的影响和刺激下发展起来的,但其青铜铸造技术并非直接来源于中原,而是来源于虎方.虎方青铜铸造技术直接来源于二里岗时期居住在湖北黄陂盘龙城的中原人.宁乡青铜文化起源于武丁时期,因武丁南征而兴起.古三苗国以宁乡炭河里为中心从殷墟二期开始创造了灿烂的青铜文明.     

中国沿海和内陆强震前地表潜热通量异常特征

地表潜热通量(SLHF)是反映地表蒸发能量大小的大气参数。国外学者分析近年若干沿海地区的强震时发现,沿海强震震中附近在震前出现了潜热通量异常,而远离海域的内陆地震并未出现潜热通量异常。本文以近10年发生在中国的1次沿海强震和3次内陆强震为例,进行了震前SLHF异常的时空特征分析,发现震前3～10d,震中附近均出现了SLHF异常。该异常主要分布在震中及其周围地区,且与区域内的主要活动构造带走向一致。因此,SLHF异常可以成为一种有价值的大地构造活动性遥感监测指标,并为中国沿海和内陆地区强震提供有用的临震前兆信息。     

Characterization of an endo-1,4-β-glucanase from Lilium longiflorum that functioned in pollen germination and pollen tube growth

Endo-β-glucanases play vital roles in the regulation of pollen tube growth. Here, a previously identified endo-l,4-β-glucanase from Lilium Iongiflorum （lily）, named LlpCell, was expressed in Escherichia coli, purified, and further investigated for its physiological function. The recombinant LlpCell protein hydrolyzed carboxy-methylcellulose （CMC） and exhibited activity towards laminarin from Eisenia. arborea and 1,3：l,4-β-glucan of barley. The pH for the optimum activity was 6.0 and the value of Km calculated from CMC was 5.0 mg/mL. Adding EDTA resulted in the total loss of the enzymatic activity, and this effect could be restored by the addition of Ca^2＋. Western blotting analysis showed that LlpCell protein was present in pollen grains and rehydrated pollen grains, and the amount of the protein was increased during pollen germinating, but not in the pollen tube. Consistently, the immunofluorescence labeling study with the antibody against LIpCell also indicated the presence of LIpCell at the begin-ning of germination, but not in the elongating pollen tube. Furthermore, incubation of LlpCell with pollen at the beginning of pollen germination increased the germination percentage and the length of pollen tube. All of these results suggested that LlpCell could play an important role in the regulation of lily pollen germination and the initiation of pollen tube growth.     

Study on cellular internalization of poly（vinyldiaminotriazine）-based hydrogen bonding type non-viral transgene vector

Previously we successfully prepared poly（vinyldiaminotriazine）（PVDT）-based non-viral vectors which complexed piasmid DNA via hydrogen bonding with adenine-thymine base pairs, in this report, surface charges and complex sizes of this system were further examined. The results showed that PVDT-based polymer could cover surface charges of DNA resulting in slightly negative or neutral complexes. It was also found that the complex sizes were governed by two events： the aggregation induced by the instability of neutral particles, and more compact complexes produced by PVDT-based polymers. In the study of cellular uptake, chlorpromazine and filipin III were used to inhibit clathrin-and caveolae-mediated endocytosis, respectively. We found that PVDT-based systems were transported into cells via a non-clathrin, non-caveolae mediated endocytosis. This special process was studied by temperature inhibition and kinetics assays. It was revealed that such a pathway was characterized by （i） a more en- ergy dependent process and （ii） a much slow transfection-effective internalization.     

Molten-salt synthesis of tungsten oxide nanotubes： Morphological and gas sensitivity

A very simple molten-salt method was used to synthesize WO3 nanotubes at moderate temperature （600 ）. The outer and inner diameters of the WO3 nanotubes were about 100 nm and 30 nm,with a length of dozens of micrometers. TEM and XRD analyses showed that the WO3 nanotubes were single crystal, and had monoclinic structure with the axes preferentially aligned along the [011] direction. The photoluminescence and the gas sensitivity of the nanotubes had been tested, showing that the products very well responded to acetone at 340℃.     

新疆野生甘草与栽培甘草的质量特征比较

为比较新疆野生甘草与栽培甘草的质量差异,本文采用粉末X射线衍射(XRD)全谱分析法和紫外分光光度法对二者进行对比分析。结果表明:野生甘草和栽培甘草在2θ值2°～30°低角度范围内指纹图谱无较大特征差异;野生甘草中主要化学成分和Fe、Mg、Ca三种微量元素的含量均高于栽培甘草,且成份比例也不同——野生甘草中三种微量元素的含量比例是1.00:6.67:107.84,而栽培甘草中为1.00:8.70:36.42。     

Localization of calmodulin and calmodulin-like protein and their functions in biomineralization in P. fucata

Calmodulin (CaM) and calmodulin-like protein (CaLP) are two proteins involved in biomineralization. Their localizations in Pinctada fucata mantle epithelia were studied by Western blot (WB) analysis of the nuclear/cytosol fraction of primary cultured Pinctada fucata mantle cells and immunogold electron microscopy. The results showed a completely different distribution of these two proteins at the subcellular level. CaM was distributed throughout both the nucleus and cytoplasm of the mantle epithelium but CaLP was distributed only in the cytoplasm. The functions of these two proteins in biomineralization were investigated by shell regeneration. During this process, the expressions of CaM and CaLP were greatly enhanced in different organelles of the mantle epithelium. Overexpression of these two proteins and a mutant of calmodulin-like protein (M-CaLP) that lacks an extra C-terminal tail in MC3T3-E1 promoted the mRNA expression of osteopontin, a biomineralization marker for osteoblasts. All of the results indicated that CaM and CaLP have completely different distributions in the mantle epithelium and affect the biomineralization process at different levels. The extra C-terminal tail of CaLP is important for its functions in biomineralization in Pinctada fucata.     

实行列式的几个不等式

给出实矩阵的几个不等式,这些不等式推广了[1-4]中的若干结论.     

古诗词英译中意象的传递——从两首古诗英译看动态意象和静态意象的传递

意象是古诗词的灵魂,是赏析和翻译古诗词的关键.为了使原文和译文达到一样或类似的审美效果,译者要对意象做适当的处理.在翻译古诗词的过程中,原文中的意象应尽量保留.当保留原文意泉可能引起译文读者误解或转交意象可以更好地体现原文时,需做适当的转换.