浙江沿海虾蛄生物学及其开发利用研究报告

本文根据浙江省教委、省基金、舟山市科委关于浙江沿海虾蛄生物学及其开发利用研究的要求，对四年多来的研究进行总结，主要包括资源与分布、生物学习性、暂养及运输技术、营养成份分析、开发利用等诸方面     

溶剂化缔合物模型与溶解度

提出了一种适合于研究非理想溶液的溶剂化缔合物模型.借助该模型,对物质的溶解度在定量和半定量的水平上进行了研究,并获得了满意的结果。     

用栅板剪切干涉法确定应力强度因子

本文介绍了一种用栅板剪切干涉法确定应力强度因子的新方法,阐明了该方法的基本原理与实验技术,通过三点弯曲梁确定了Ⅰ型裂纹应力强度因子,实验结果与理论上近似计算结果相符合。     

三峡二期围堰工程几个问题的研究及实践

三峡工程二期围堰是三峡工程最重要的临时建筑物之一，大江截流水深大，流量大；防渗墙深度大，工程量大，工期紧，且还要穿过各种复杂地层，通过试验，研究，采取有效地对策确保大江安全，顺利截流，基坑按三峡总工期目标要求抽干，检查渗漏量仅为设计估计量的1／10。     

光学系统自动设计中应用动态规划的个算法

本文用动态规划方法求解具有等式和不等式约束的光学系统的最优化问题,以Kuhn-Tucker条件为基础,利用牛顿迭代法提出最优决策的一个算法,并证明其局部收敛性。     

用微弹轰击法将GUS基因导入香蕉茎尖组织细胞

用在弹轰击法将外源ＤＮＡ导入香蕉（Ｍｕｓａ，ｓｐｐ）茎尖细胞，以ＧＵＳ基因作为报告基因，用Ｘ－Ｇｌｕｃ浴液对ＧＵＳ基因表达产物进行组织化学鉴定。样品材料细胞中发现有明显的蓝色斑点，而对照则没有。实验说明用微弹轰击法可以将外源基因导入香蕉茎尖组织并在其中成功表达，这为香蕉的外源基因导入工作提供了新的有效途径．     

Millikan′s油滴实验数据处理透析

本文从误差传递的角度出发，代入合理的数据，来说明目前高校实验中对此内容的数据处理的问题所在以及应怎样采取措施解决。     

钻柱系统振动测试和分析

利用应变和压电测试系统以及随机信号和振动分析系统,在模型和试验钻机上,进行了钻柱系统的固有频率、响应、钻头的动态压力、功率谱和动态传递函数等测试分析。在横向振动支承条件,岩石的刚度以及动态碎岩机理等方面得到了一些有益的研究结果,并提出了岩石刚度动态测定法。以上研究为理论分析提供了依据。     

Application of the Constrained Predicated Nets and Interval Logic to Production Systems

1 .INTRODUCTIONDuetotheireconomicsignificanceandclosenesstopersons,productionsystemsmustbemodeledbeforetheirimplementationanduse,thus,amethodwhichspecifies ,analyzesandsynthesizestheproductionsys tems—discreteeventsystems (DES)hasbeenestab lishedanddevelopedstepbystepinrecentyears.DESmethodspecifiestheplantbasedonitsdiscretecharacters,however,intherealworld ,therearefewplantsthatcanbemodeledbypurelydiscretemathe maticalmodelbecausetherearealwayssomecontinu ousparametersforstudyinnearlya…     

A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.