用mRNA差异显示技术分离盐胁迫下小麦耐盐相关cDNA

目的用mRNA差异显示技术分离小麦盐胁迫应答cDNA,为进一步研究小麦耐盐机理奠定基础。方法将小麦(Triticum aesticum L.)耐盐品系宝丰7228r种子分别置于含NaC l 0‰和12‰的Hoagland培养液中生长,7d后分别取叶片,提取总RNA并分离出其中的mRNA,通过锚锭引物O ligo dT10GC反转录和9个10核苷酸随机引物进行PCR扩增。结果DDRT-PCR结果显示,有4个差异DNA片段只在盐胁迫的小麦耐盐品系基因组中表达,而在对照中没有出现。这4个差异cDNA片段分别命名为ts01(260 bp),ts02(330 bp),ts03(420 bp),ts04(600 bp)。4个差异cD-NA片段的RNA杂交结果显示,只有ts04存在明显差异,在盐胁迫条件下有杂交斑点而在对照中没有杂交斑点,其余3个cDNA片段在盐胁迫和对照中都没有杂交斑点。进一步将ts04克隆并进行DNA序列测定及同源性分析。结论ts04可能是耐盐相关cDNA片段,该片段核酸序列与麦属抗性相关的肌动蛋白基因有23%同源。     

面向组件的仿真系统构建方法研究

随着软件组件技术的不断发展,基于组件的软件开发方法在仿真领域逐渐被接受,特别是基于分布式的仿真软件开发。SISO（仿真互操作标准组织）提出的BOM（基本对象模型）通过标准化规范描述面向组件的仿真模型,为构建具有可扩展、可组合和可互操作的仿真系统提供了面向组件的实现技术。首先,描述BOM的模板结构及其体系结构,设计基于BOM的仿真模型组件结构,分析这种结构的仿真模型组件间的互操作性;接着从概念层次、联邦成员层次和联邦层次三个不同层次提出面向组件的仿真系统构建方法;最后讨论一种通用的仿真运行框架,通过组合不同的仿真模型组件实现仿真系统的快速构建。

Abstract：

With the development of the software component technique, the components based software development method is accepted in the simulation domain and specifically in the distributed simulation’s software development. The standard of Base Object Model （BOM） developed by SISO （Simulation Interoperability and Standard Organization） is the component-oriented specification of simulation models which gives a component-oriented technique to construct extensible composable and interoperable simulation systems. Firstly, the template and architecture of BOM were described based on which the structure of simulation model components were designed and the interoperation between them were analyzed; Secondly, the processes for constructing simulation systems were argued from the concept level and the federate level to the federation level; Lastly, by composing various of simulation model components, the general simulation running framework which was argued provided component-oriented capabilities to rapidly building simulation systems.