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981.
HAb18G/CD147-mediated calcium mobilization and hepatoma metastasis require both C-terminal and N-terminal domains 总被引:4,自引:0,他引:4
Jiang JL Chan HC Zhou Q Yu MK Yao XY Lam SY Zhu H Ho LS Leung KM Chen ZN 《Cellular and molecular life sciences : CMLS》2004,61(16):2083-2091
HAb18G/CD147 is a heavily glycosylated protein containing two immunoglobulin superfamily domains. Our previous studies have indicated that overexpression of HAb18G/CD147 enhances metastatic potentials in human hepatoma cells by disrupting the regulation of store-operated Ca2+ entry by nitric oxide (NO)/cGMP. In the present study, we investigated the structure-function of HAb18G/CD147 by transfecting truncated HAb18G/CD147 fragments into human 7721 hepatoma cells. The inhibitory effect of HAb18G/CD147 on 8-bromo-cGMP-regulated thapsigargin-induced Ca2+ entry was reversed by the expression of either C or N terminus truncated HAb18G/CD147 in T7721C and T7721N cells, respectively. The potential effect of HAb18G/CD147 on metastatic potentials, both adhesion and invasion capacities, of hepatoma cells was abolished in T7721C cells, but not affected in T7721N cells. Release and activation of matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were found to be enhanced by the expression of HAb18G/CD147, and this effect was abolished by both truncations. Thapsigargin significantly enhanced release and activation of MMPs (MMP-2 and MMP-9) in non-transfected 7721 cells, and this effect was negatively regulated by SNAP. However, no effects of thapsigargin or SNAP were observed in T7721 cells, and expression of HAb18G/CD147 enhanced secretion and activation of MMPs at a stable and high level. Taken together, these results suggest that both ectodomain and intracellular domains of HAb18G/CD147 are required to mediate the effect of HAb18G/CD147 on the secretion and activation of MMPs and metastasis-related processes in human hepatoma cells by disrupting the regulation of NO/cGMP-sensitive intracellular Ca2+ mobilization although each domain may play different roles.Received 1 April 2004; received after revision 15 June 2004; accepted 22 June 2004 相似文献
982.
Zhang J Gray J Wu L Leone G Rowan S Cepko CL Zhu X Craft CM Dyer MA 《Nature genetics》2004,36(4):351-360
The retinoblastoma protein (Rb) regulates proliferation, cell fate specification and differentiation in the developing central nervous system (CNS), but the role of Rb in the developing mouse retina has not been studied, because Rb-deficient embryos die before the retinas are fully formed. We combined several genetic approaches to explore the role of Rb in the mouse retina. During postnatal development, Rb is expressed in proliferating retinal progenitor cells and differentiating rod photoreceptors. In the absence of Rb, progenitor cells continue to divide, and rods do not mature. To determine whether Rb functions in these processes in a cell-autonomous manner, we used a replication-incompetent retrovirus encoding Cre recombinase to inactivate the Rb1(lox) allele in individual retinal progenitor cells in vivo. Combined with data from studies of conditional inactivation of Rb1 using a combination of Cre transgenic mouse lines, these results show that Rb is required in a cell-autonomous manner for appropriate exit from the cell cycle of retinal progenitor cells and for rod development. 相似文献
983.
GongZhu-qian ZhuGuo-qiang 《武汉大学学报:自然科学英文版》2004,9(3):319-322
In this paper, a two-dimensional (2-D) finitedifference time-domain method (FDTD) scheme is used to simulate the transient scattering characteristics of buried objects, which are modeled by columns of arbitrary permittivities, conductivities, and sizes. The FDTD soil is modeled by isotropic, homogeneous and lossy media. The standing-traveling wave boundary condition (STWBC) that can simplify calculation and save CPU storage is used for modeling physical absorbers inside the FDTD computational dornairL Reflection of electromagnetic pulses incident on a layered medium and transient scattering by the ground and an underground airsqu are cylinder are computed. These results verify the validity of the FDTD scheme by comparisons with those shown in some references. Numerical results presented in the final part of this paper are desirable and meaningful, explicitly distinguishing echo waves stemming from the ground and the buried ohjects. 相似文献
984.
Twenty Yongzheng copper coins were fast dat-ing by X-ray energy dispersive spectrometer with scanning electron microscope (SEM-EDS) according to official ingredient. The 20 coins were classified in two groups according to the results. Group 1 represents the coins minted before AD 1 728 and group 2 represents the coins minted after AD 1 728. At concentration range (0.00%-5.13%), the distribution of lead is not homogeneous in the coins, and the largest diameter of lead inclusion is less than 15 μm. The official fineness degrade is supported. 相似文献
985.
Objective: To investigate the in-vitro antitumor immune responses of dendritoma formed by mouse hepatocellular carcinoma (HCC) cells and lymphotactin (Lptn) gene modified dendritic cells (DCs). Method: DCs prepared from mouse bone marrow were genetically modified by lymphotactin adenovirus, and fused with H22 cells by polyethylene glycol (PEG). RT-PCR and ELISA were employed to identify lymphotactin expression at mRNA and protein level. Cell phenotypes and fusion efficiency was detected by FACS. The stimulatory effect of DC on T cells was detected by mixed lymphocyte reaction. The cytotoxicity activity against H22 cells was assayed by LDH method. Results: Lymphotactin could be efficiently expressed by DCLptn/H22 hybridoma. DCLptn/H22 cells could induce potent T cell proliferation effect and generate strong cytotoxic T lymphocyte (CTL) reaction against allogenic H22 cells. Conclusion: Lymphotactin genetic modification could enhance the in vitro immune activity of the dendritoma. 相似文献
986.
TCP-Rab: a receiver advertisement based TCP protocol 总被引:3,自引:0,他引:3
With the evolution of network technologies, the deficiencies of TCP protocol are becoming more and more distinct. The new TCP implementation, called Receiver Advertisement Based TCP (TCP-Rab) proposed here to eliminate these deficiencies, adopts two basic mechanisms: (1) Bandwidth Estimation and (2) Immediate Recovery. Bandwidth estimation is carried out at the receiver, and the result is sent back to the sender via the acknowledgements. Immediate Recovery guarantees high performance even in lossy link. Rab can distinguish the reason for packet loss, and thus adopt appropriate recovery strategy. For loss by network congestion, it will back off its congestion window, and for loss by link errors, it will recover the congestion window immediately. Simulations indicated that Rab has superiority over other TCP implementations. 相似文献
987.
MRS study on lentiform nucleus in idiopathic Parkinson‘s disease with unilateral symptoms 总被引:5,自引:0,他引:5
OBJECTIVE: To investigate changes in magnetic resonance spectroscopy (MRS) of lentiform nucleus during the early stage of Parkinson's disease. METHODS: Twenty-five patients with idiopathic Parkinson disease with unilateral symptoms (IPDUS) and 25 healthy volunteers were enrolled in this study. MRS of the lentiform nucleus in each patient was taken and then concentrations of N-acetylaspartate (NAA), Creatine (Cr) and Choline (Cho) were calculated. RESULTS: Compared to that in the control, NAA/ (Cho+Cr) was significantly lower in the lentiform nucleus contralateral to symptoms and even that in the ipsilateral side in IPDUS patients (all P<0.05); while there was no difference between the two sides in the healthy volunteer (P>0.05). The ratio of NAA/(Cho+Cr) ipsilateral to the sympatomatic side of the patient was also lower than that of the control (P<0.05). CONCLUSIONS: There might be some changes with MRS on the lentiform nucleus during the early stage of idiopathic Parkinson's disease with unilateral symptom. MRS may be one of the reliable methods for early or even sub-clinical diagnosis. 相似文献
988.
In this work, the charge dynamics characteristics of injection, transport and decay in porous and non-porous polytetrafluoroethylene (PTFE) film electrets were investigated by means of corona charging, isothermal and thermal stimulating surface-potential decay measurements. The results showed that the initial surface potential, whether positively or negatively charging, is much higher in non-porous PTFE than in porous PTFE. For porous film the value of initial surface potentials increases with increase of film thickness. Higher charging temperature can remarkably improve charge stability. The charge dynamics are correlated to materials microstructure according to their scanning electron micrographs. For non-porous PTFE films, polarizability change of C-F bonds is the main origin of electret charges; but for porous PTFE film a large number of bulk and interface type traps are expected because of the greater area of interface and higher crystallinity. 相似文献
989.
Science Letters:On stochastic optimal control of partially observable nonlinear quasi Hamiltonian systems 总被引:4,自引:0,他引:4
A stochastic optimal control strategy for partially observable nonlinear quasi Hamiltonian systems is proposed. The optimal control forces consist of two parts. The first part is determined by the conditions under which the stochastic optimal control problem of a partially observable nonlinear system is converted into that of a completely observable linear system. The second part is determined by solving the dynamical programming equation derived by applying the stochastic averaging method and stochastic dynamical programming principle to the completely observable linear control system. The response of the optimally controlled quasi Hamiltonian system is predicted by solving the averaged Fokker-Planck-Kolmogorov equation associated with the optimally controlled completely observable linear system and solving the Riccati equation for the estimated error of system states. An example is given to illustrate the procedure and effectiveness of the proposed control strategy. 相似文献
990.
Jinsong Li Dayuan Chen Zhiming Han Ziyu Zhu Duancheng Wen Qingyuan Sun Zhonghua Liu Minkang Wang Li Lian Jun Du Pengyan Wang Hemin Zhang 《科学通报(英文版)》2002,47(6):467-469
Interspecies somatic nuclear transfer (NT) may provide a new approach for preservation of the endangered rare species. Previous
interspecies cloning studies have shown that a nucleus from a quiescent somatic cell supports early development of reconstructed
embryos in the ooplasm from another species. In this study, we transferred nonquiescent somatic cells from a giant panda into
the perivitelline space of the enucleated rabbit oocytes. After electrofusion (at the rate of 71.6%) and electrical activation,
4.2% of the panda-rabbit reconstructed embryos developed to blastocyst in vitro. For improving the development rate of reconstructed embryos, we used serial NT in this study, i.e. blastomeres from reconstructed
morulae were transferred into the perivitelline space of the enucleated rabbit oocytes. The fusion rates in the groups of
serial I, serial II and serial III were 79.5%, 84.1% and 78.0%, respectively, having no difference with that of somatic group.
And the blastocyst rates in serial NT groups were 19.4%, 13.5% and 10.3%, respectively, which are significantly higher than
that in somatic NT group. These results indicate that the nuclei from nonquiescent somatic cells can support early development
of reconstructed embryos and serial NT can improve the development rate of interspecies reconstructed embryos.
These authors contributed equally to this work. 相似文献