Prophylaxis of tumor through oral administration of IL-12 GM-CSF gene carried by live attenuated salmonella

A live attenuated AraA^- autotrophic mutant ofSalmonella typhimurium (SL3261) was used as carrier for eukaryotic expression vectors EGFPN1, pCMVmIL-12, pCMVhIL-12, pCMVmGM-CSF and pCMVhGM-CSF and was administered orally to BALB/c and C57BL/6 mice. After 6 weeks, these mice were challenged with 4T1 and Lewis tumor cells respectively. GFP expression and gene integration could be detected in mice’s livers, spleens, intestines, kidneys and tumors. The serum level of cytokines increased significantly in treated mice, so did the ratio of CD ₈ ⁺ /CD ₄ ⁺ , which resulted in the tumor regression and prolongation of the survival time of those mice. These researches laid an experimental foundation for the tumor gene therapy using live attenuated salmonella.     

Sex-related gene and sex identification of Crested IbisNipponia nippon (Ciconiiformes: Threskiornithidae)

The Crested Ibis (Nipponia nippon) is a critical endangered species of the world. At present, a reintroduction program will be conducted to save this species essentially. However, because the Crested Ibise is a sexual alike bird, it is very difficult to identify the sex from the morphological character. In order to identify the sex easily and select the right individuals for captive breeding and reintroduction, the sex related gene on W chromosome was amplified and the sex of three Crested Ibises were also identified in the present study. The 262bp fragment was also sequenced, and we found that there were 13 different nucleotide sites with 2.25 of transition/transversion based on the comparison with that of the Oriental White Stork. The sequence will also provide a theoretic base for further designing specific primer for sex related gene in the Crested Ibis.     

Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18.

Salmonella enterica serovar Typhi (S. typhi) is the aetiological agent of typhoid fever, a serious invasive bacterial disease of humans with an annual global burden of approximately 16 million cases, leading to 600,000 fatalities. Many S. enterica serovars actively invade the mucosal surface of the intestine but are normally contained in healthy individuals by the local immune defence mechanisms. However, S. typhi has evolved the ability to spread to the deeper tissues of humans, including liver, spleen and bone marrow. Here we have sequenced the 4,809,037-base pair (bp) genome of a S. typhi (CT18) that is resistant to multiple drugs, revealing the presence of hundreds of insertions and deletions compared with the Escherichia coli genome, ranging in size from single genes to large islands. Notably, the genome sequence identifies over two hundred pseudogenes, several corresponding to genes that are known to contribute to virulence in Salmonella typhimurium. This genetic degradation may contribute to the human-restricted host range for S. typhi. CT18 harbours a 218,150-bp multiple-drug-resistance incH1 plasmid (pHCM1), and a 106,516-bp cryptic plasmid (pHCM2), which shows recent common ancestry with a virulence plasmid of Yersinia pestis.     

心理干预对维持性血液透析患者影响的随机对照研究

将77例维持性血液透析患者随机分为干预组或对照组。对照组接受常规的血液透析并按医嘱服用重组人促红细胞生成素（rhEPO）;干预组在此基础上,还接受渐进性肌肉放松训练和脑波同步治疗。采用SCL-90量表和SF-36问卷,在干预前、后3天内分别对两组进行测量,同时检测血常规、肾功能及透析间期体重增加量与干体重的比值(IWGR%)。70例患者（干预组36例,对照组34例）完成了实验。结果发现,经过心理干预,干预组的心理健康状况、总体生活质量、血红蛋白（HB）、红细胞计数（RBC）、红细胞压积（HCT）以及透析前血清钙(Ca²⁺)显著高于对照组;透析前血清尿素氮（BUN）、磷 (PO^3-₄)、钾（K⁺）水平以及透析间期体重增加量与干体重的比值（IWGR%）显著低于对照组;两组透析前的血清肌酐（Scr）差异无显著性意义。     

金黄色葡萄球菌SarA蛋白原核表达及免疫原性研究

目的 构建SarA的原核表达载体,对SarA蛋白免疫原性进行初步研究.方法 首先应用生物信息学方法 对SarA基因进行分析;以金黄色葡萄球菌基因组为模板,通过PCR扩增、酶切、连接等一系列反应进行原核重组质粒的构建;IPTG诱导原核重组质粒表达,对SarA蛋白进行纯化、浓缩,将浓缩后的蛋白与佐剂混合免疫家兔,收集血清,...     

基于幂函数控制指令的垂直飞行仿真性能分析

基于模态直接切换的飞行高度控制方法,在输入控制指令后,存在控制量输出震荡,甚至发散的问题。幂函数符合飞行高度指令变化规律,以此为原型函数,推导给定高度,当前高度和垂直速度之间的关系,将这一变换关系作为垂直速度控制回路的输入指令,设计了平稳控制系统,该系统不需要进行控制模态切换,就可以实现飞行高度保持,以及飞行高度的改变。通过A320飞行仿真平台对设计的平稳控制系统进行性能分析,结果表明该系统可以实现高度的平稳控制,且稳态误差得到了很好抑制。     

建立我国公司治理结构问题的探讨

本文深入分析了公司治理结构的内涵，比较分析了国外几种典型的公司治理结构模式，并就建立我国公司治理结构的目标、途径等问题进行了探讨     

Laplace变换的数值反演算法的研究

利用交错级数的加速方法以及修正的Bessel-Gauss求和公式对满足一定条件的函数的Laplace变换的数值反演进行讨论，给出了一个条件较为有效的数值计算方法。