BE—8347—14固定液对水中痕量酚的分离性能的研究

本文用BE—8341—14固定液对松花江水中痕量酚的测定,并对其在气相色谱中的分离性能以模式水样进行了研究。其结果表明,该固定液为实际水样中痕量酚的测定提供了新的参考方法。     

沿海防护林体系海陆风环流的近地面层风温湿廊线特征

利用系留气球观测系统观测了沿海防护林体系海陆风环流时近地面的风、温、湿廊线特征，有如下结论：（１）海防林体系海陆风廓线均呈单峰形，但海风的厚度比陆风大；（２）海防林体系对近地面层的海陆风有较明显的减弱作用，但对陆风的影响高度比对海风的影响高，在林带高度附近风速降低最多，１～１．５Ｈ可降低５０％～６０％，１．５～５Ｈ可降低２０％～３０％，（３）对近地面层有一定的增温增湿作用，防风环流时１ｍ高度可增温约１℃，增加比温１．０ｇ／ｋｇ。     

聚丙烯酸酯核／壳乳液膜的应力松弛

合成了阴离子乳化剂含量不同的５种聚丙烯酸酯核／壳乳液，分别成膜，用岛津ＡＧ－Ａ材料试验机，进行了５种膜的应力松弛试验，求出了应力松弛模量和应力松弛函数，应力松弛函数形式为Ｅ＝Ａｔ＾Ｂ，并讨论了成膜厚度对应力松弛数据的影响。     

染污绝缘子在交流电压下的污闪条件分析

沿染污绝缘表面的交流电弧在每周内强度交变，甚至当电流过零时发生熄灭和重燃。决定交流污闪条件的最重要的因素是交流电弧的恢复条件，而不是以往人们所认为的重燃条件。本文对交流电弧的恢复条件作了理论分析和试验验证，并提出了计算染污绝缘子交流污闪条件的数学模型，理论计算结果和试验结果是相符的。     

Compensatory caspase activation in MPP+-induced cell death in dopaminergic neurons

Many have hypothesized that cell death in Parkinsons disease is via apoptosis and, specifically, by the mitochondrial-mediated apoptotic pathway. We tested this hypothesis using a mouse dopaminergic cell line of mesencephalic origin, MN9D, challenged with the Parkinsonism-causing neurotoxin MPP⁺ (1-methyl-4-phenylpyridinium ion). Apoptosis was the main mode of cell death when the cells were subjected to MPP⁺ treatment under serum-free conditions for 24 h. Caspase-3 and caspase-9, however, were not activated, thus indicating the existence of alternate or compensatory cell death pathway(s) in dopaminergic neuronal cells. Using caspase inhibitors, we demonstrated that these pathways involve caspase-2, –8, –6 and –7. A time-course study indicated that activation of caspase-2 and –8 occurred upstream of caspase-6 and caspase-7. Upon MPP⁺ challenge, the apoptosis-inducing factor was translocated from the mitochondria into the MN9D cytosol and nucleus. These results suggest the existence of alternative apoptotic pathways in dopaminergic neurons.Received 20 September 2004; received after revision 5 November 2004; accepted 22 November 2004     

Dysregulation of the TSC-mTOR pathway in human disease

The mammalian target of rapamycin (mTOR) has a central role in the regulation of cell growth. mTOR receives input from multiple signaling pathways, including growth factors and nutrients, to stimulate protein synthesis by phosphorylating key translation regulators such as ribosomal S6 kinase and eukaryote initiation factor 4E binding protein 1. High levels of dysregulated mTOR activity are associated with several hamartoma syndromes, including tuberous sclerosis complex, the PTEN-related hamartoma syndromes and Peutz-Jeghers syndrome. These disorders are all caused by mutations in tumor-suppressor genes that negatively regulate mTOR. Here we discuss the emerging evidence for a functional relationship between the mTOR signaling pathway and several genetic diseases, and we present evidence supporting a model in which dysregulation of mTOR may be a common molecular basis, not only for hamartoma syndromes, but also for other cellular hypertrophic disorders.     

Screening of the interacting proteins with NifA in <Emphasis Type="Italic">Azospirillum brasilense</Emphasis> Sp7 by the yeast two-hybrid system

NifA in AzospiriUum brasilense plays a key role in regulating the synthesis and activity of nitrogenase in response to ammonia and oxygen available. In this work we used the yeast two-hybrid system to identify the proteins that interact with NifA. The nifA gene was fused to the yeast two-hybrid vector pGBD-C2, and three A. brasilense Sp7 genomic libraries for use in yeast two-hybrid studies were constructed. Screening of the libraries identified four clones encoding proteins that interact with NifA. The confirmation of the interactions of each gene product of the four clones and NifA were carried out by exchanging the vectors for nifA and the four clones and by mutageneses of the four clones with shift reading frame experiments in yeast two-hybrid studies. DNA sequence analyses showed that two clones encode proteins containing PAS domains that play an important role in signal transduction. One clone has high similarity with the fhuE gene of Escherichia coli, whose gene product is involved in iron uptake and transportation, and the other clone encodes an unknown protein.     

战术导弹仿真技术的发展与展望

本文论述了仿真技术在战术导弹研制中的重要地位和作用。概述了国外仿真技术在战术导弹上的应用情况以及有关的仿真设备。文中结合我国的实际,提出了当前战术导弹仿真技术面临的几个问题,并就它们的解决途径提出了一些看法和建议。