Identification of a novel DNA molecule associated with Tobacco leaf curl virus

A circular DNA molecule, designated as DNAβ, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAβ sequences of whitefly-transmitted geminiviruses (WTGs). The complete nucleotide sequences of DNAβ of Y5 and Y8 (TLCV DNAβ) were determined. Y5 DNAβ comprises 1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNAβ consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA. TLCV DNAβ has little sequence homology to DNA-A of TLCV., except that it shares conserved TAATATTAC loop sequence with TLCV DNA-A. Sequence comparison showed that Y5 DNAβ shared 85% sequence homology with Y8 DNAβ, and both Y5 DNAβ and Y8 DNAβ had relatively low sequence identity (51%–65%) with the reported DNAβ molecules associated with Ageratum yellow vein virus and Cotton leaf curl virus. The immunotrapping PCR and whitefly transmission tests showed that DNAβ molecule could be encapsidated in virus particle and transmitted by Bemisia tabaci. This is the first report of DNAβ associated with WTGs in China.     

The 1997–1998 warm event in the South China Sea

A strong warm event happens during spring 1997 to spring 1999 in the South China Sea. Its intensity and duration show that it is the strongest event on the record over the past decades. It also corresponds with the severe flood over the valley of the Yangtze River and a couple of marine environmental events. This note addressed the evolution process by using several data sets, such as sea surface temperature, height and wind stress in addition to subsurface temperature. The onset of the warm event almost teleconnects with the El Niño event in the tropical Pacific Ocean. Summer monsoon is stronger and winter monsoon is weaker in 1997 so that there are persistent westerly anomalies in the South China Sea. During the development phase, the warm advection caused by southerly anomalies is the major factor while the adjustment of the thermocline is not obvious. Subsequently, the southerly anomalies decay and even northerly anomalies appear in the summer of 1998 resulting from the weaker than normal summer monsoon in 1998 in the South China Sea. The thermocline develops deeper than normal, which causes the downwelling pattern and the start of the maintaining phase of the warm event. Temperature anomalies in the southern South China Sea begin to decay in the winter of 1998–1999 and this warm event ends in the May of 1999.     

逆变器预置开关角误差对输出谐波的影响

针对数字控制技术的特点，分析了在选择性谐波消除法的理论计算结果与实际开关角数字控制结果出现偏差时，对逆变器输出电压谐波含量的影响。并研究了开关角数量对逆变器性能的影响，结果表明：开关角控制误差对逆变器输出谐波有一定影响，但可以在计算时修正；而开关角数量的增加有利于减小电流脉动，提高输出电压质量，但开关频率随之增加。     

略论庄子散文的艺术性

庄子散文大多以丰富多彩的寓言构成。他擅长讲故事，善于刻画人物，文笔辛辣，气势雄伟，意境开阔，笔法多样，行文千变万化，他善于比喻，富于变化，含蓄蕴藉，生动活泼，是中国文学史上的语言大师。     

用Barkhausen杂讯研究中碳钢的低周疲劳损伤特性

用磁无损检测技术中的Barkhausen杂讯法评估中碳钢的底周疲劳过程，表明Barkhausen杂讯的幅值B随循环周次N的增长而变化，可以利用B和N的关系曲线来评估材料的损伤特性.     

浅谈燃气采暖热水炉的概念及其检验标准

从中国加入世贸组织引起燃气行业标准改革入手，引出燃气采暖热水炉的概念，燃气采暖热水炉标准的编写势在必行，并阐述了燃气采暖热水炉的新旧产品检验标准内容要求的不同之处。     

网上CAI系统技术

本文在总结网上CAI已有成果的基础上，概述了未未网上CAI的特点，并提出了网上CAI系统设计的一些想法，指出了尚待研究的一些问题。     

百色起义纪念馆馆舍规划设计

通过对百色起义纪念馆所处环境的分析，提出建设百色市历史文化长廊的规划设想，确定“一轴线，二核心，一序列”的规划结构，在此基础上，介绍了具有强烈时代感和个性特征的百色起义纪念馆馆舍的规划设计。