Bose-Einstein condensation of atomic gases

The early experiments on Bose-Einstein condensation in dilute atomic gases accomplished three long-standing goals. First, cooling of neutral atoms into their motional ground state, thus subjecting them to ultimate control, limited only by Heisenberg's uncertainty relation. Second, creation of a coherent sample of atoms, in which all occupy the same quantum state, and the realization of atom lasers - devices that output coherent matter waves. And third, creation of a gaseous quantum fluid, with properties that are different from the quantum liquids helium-3 and helium-4. The field of Bose-Einstein condensation of atomic gases has continued to progress rapidly, driven by the combination of new experimental techniques and theoretical advances. The family of quantum-degenerate gases has grown, and now includes metastable and fermionic atoms. Condensates have become an ultralow-temperature laboratory for atom optics, collisional physics and many-body physics, encompassing phonons, superfluidity, quantized vortices, Josephson junctions and quantum phase transitions.     

Selective imprinting of gut-homing T cells by Peyer's patch dendritic cells

Whereas naive T cells migrate only to secondary lymphoid organs, activation by antigen confers to T cells the ability to home to non-lymphoid sites. Activated effector/memory T cells migrate preferentially to tissues that are connected to the secondary lymphoid organs where antigen was first encountered. Thus, oral antigens induce effector/memory cells that express essential receptors for intestinal homing, namely the integrin alpha4beta7 and CCR9, the receptor for the gut-associated chemokine TECK/CCL25 (refs 6, 8, 9). Here we show that this imprinting of gut tropism is mediated by dendritic cells from Peyer's patches. Stimulation of CD8-expressing T cells by dendritic cells from Peyer's patches, peripheral lymph nodes and spleen induced equivalent activation markers and effector activity in T cells, but only Peyer's patch dendritic cells induced high levels of alpha4beta7, responsiveness to TECK and the ability to home to the small intestine. These findings establish that Peyer's patch dendritic cells imprint gut-homing specificity on T cells, and thus license effector/memory cells to access anatomical sites most likely to contain their cognate antigen.     

Optimal Component Types for the Design of Construction Processes

Within the project preparation phase, experienced professionals manually map design information onto process information with the aim to develop realistic and practical schedules. Unfortunately, the mapping itself is neither part of any underlying data model nor is it supported by current scheduling tools. As a consequence the process of setting up the data model for a schedule is still not supported formally. Huhnt and Enge described a modelling technique that addresses the missing linkage between design and process information[1]. The approach makes use of so called component types. These are template sub-processes that describe the fabrication procedure of typical building components. Decomposing the building into com-ponents and assigning a component type to each component allows for formal support while scheduling. Depending on the decomposition of the building into components and the complexity of the involved component types the specification effort differs. The question about optimal component types arises: Which layout of building components and component types results in minimal specification effort? This paper presents a branch and bound algorithm to determine optimal component types. For a given schedule, which has been modelled based on component types, all possible decompositions into sub-processes are determined. During the decomposition process the encountered configurations are compared. Those with minimal specifica-tion effort are registered. Theoretical and practical examples are examined and discussed.     

Impacts of orbital forcing and atmospheric carbon dioxide on Miocene ice-sheet expansion

The processes causing the middle Miocene global cooling, which marked the Earth's final transition into an 'icehouse' climate about 13.9 million years ago (Myr ago), remain enigmatic. Tectonically driven circulation changes and variations in atmospheric carbon dioxide levels have been suggested as driving mechanisms, but the lack of adequately preserved sedimentary successions has made rigorous testing of these hypotheses difficult. Here we present high-resolution climate proxy records, covering the period from 14.7 to 12.7 million years ago, from two complete sediment cores from the northwest and southeast subtropical Pacific Ocean. Using new chronologies through the correlation to the latest orbital model, we find relatively constant, low summer insolation over Antarctica coincident with declining atmospheric carbon dioxide levels at the time of Antarctic ice-sheet expansion and global cooling, suggesting a causal link. We surmise that the thermal isolation of Antarctica played a role in providing sustained long-term climatic boundary conditions propitious for ice-sheet formation. Our data document that Antarctic glaciation was rapid, taking place within two obliquity cycles, and coincided with a striking transition from obliquity to eccentricity as the drivers of climatic change.     

Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma

Glioblastoma multiforme (GBM) is a lethal brain tumour in adults and children. However, DNA copy number and gene expression signatures indicate differences between adult and paediatric cases. To explore the genetic events underlying this distinction, we sequenced the exomes of 48 paediatric GBM samples. Somatic mutations in the H3.3-ATRX-DAXX chromatin remodelling pathway were identified in 44% of tumours (21/48). Recurrent mutations in H3F3A, which encodes the replication-independent histone 3 variant H3.3, were observed in 31% of tumours, and led to amino acid substitutions at two critical positions within the histone tail (K27M, G34R/G34V) involved in key regulatory post-translational modifications. Mutations in ATRX (α-thalassaemia/mental retardation syndrome X-linked) and DAXX (death-domain associated protein), encoding two subunits of a chromatin remodelling complex required for H3.3 incorporation at pericentric heterochromatin and telomeres, were identified in 31% of samples overall, and in 100% of tumours harbouring a G34R or G34V H3.3 mutation. Somatic TP53 mutations were identified in 54% of all cases, and in 86% of samples with H3F3A and/or ATRX mutations. Screening of a large cohort of gliomas of various grades and histologies (n = 784) showed H3F3A mutations to be specific to GBM and highly prevalent in children and young adults. Furthermore, the presence of H3F3A/ATRX-DAXX/TP53 mutations was strongly associated with alternative lengthening of telomeres and specific gene expression profiles. This is, to our knowledge, the first report to highlight recurrent mutations in a regulatory histone in humans, and our data suggest that defects of the chromatin architecture underlie paediatric and young adult GBM pathogenesis.     

伞滑刃属(Bursaphelenchus)属内分类研究

目前已经报道了伞滑刃属(Bursaphelenchus)内近百种线虫,其共同的形态特征是雄虫的尾部末端具有交合伞。在该属的分类中尚存在同物异名的问题。为此,基于其特征的复杂性,笔者提出根据线虫侧线的数目、尾乳突的排列、是否出现阴门盖、交合刺形状、外分泌孔及后部子宫分支的位置等特征,将该属分为7组,其中4条侧线组为xylophilus, okinawaensis, africanus, fungivorus, cocophilus, kevini和sexdentati;3条侧线组为eggersi, eremus, hofmanni,leoni;2条侧线组为abietinus和sinensis。73种列入上述分组中,24种因为缺乏形态学资料未能列入。交合刺不能用于分组。列入该分组的39种基于亚基核糖体DNA数据的分子树说明了该分组的合理性。另外,Parasitaphelenchinae, Aphelenchoidinae和Ektaphelenchinae亚科中28种线虫基于核糖体大亚基D1—D2区序列生成的分子树显示了它们在Aphelenchoididae科中的系统关系。