Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)

The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.     

中外低保管理面面观

目前我国城市居民最低生活保障制度的执行情况进展良好。低保政策的执行在保障下岗和失业人员基本生活及促进就业和再就业等方面发挥了明显的作用。但贫困问题是一个世界性问题 ,我们应借鉴各国在缓解贫困方面的作法 ,促进我国城市扶贫工作的顺利进行     

育种目标性状间的同一关系分析方法研究

借助集对分析理论,提出了一种新的育种目标性状间的关系分析方法,阐明了其基本原理与分析步骤,并结合小麦育种实例,介绍了这种分析方法的具体应用。在此基础上,讨论了这种分析方法的优越性及尚待改进之处。表3,参9。     

美国对非石油战略的地缘政治透视

冷战时期，非洲由于丰富的自然资源和重要的战略位置成为美苏两个超级大国争夺的重要区域。冷战结束以后，随着前苏联的解体，非洲的战略地位有所下降，美国对非洲的兴趣也日益减弱。但近几年来，美国对非洲的战略开始由冷变热。文章从政治地理角度对美国近年来对非石油战略的转变进行了分析。     

Development of Full Automatic External Welding Machine for Annular Welds of Pipeline

To meet the demands of rapid development of pipeline constructions in China, a complete set of full automatic external welding machine for annular welds of pipeline and welding procedure have been developed thereof. The system has been applied in the “West-East Pipeline Project” and its performance is proven. With this welding machine the welding parameters can be either preset before or adjusted in real time during the welding process, which avoids the blemishes of same type domestic and international products. Thus it is best propitious to in-situ construction conditions and workers‘ technical level in China. In this article the development of mechanical and control systems of this product is introduced and typical welding procedure parameters are provided.     

制备介孔复合环境材料的酸洗刻蚀工艺

探索了一种制备介孔复合环境材料的方法--破坏式造孔与有机复合相结合,使最终产品可大量吸附污染水体中的各种污染物并具有一定的杀菌功能.该方法通过酸洗刻蚀,达到了去除原材料中的杂质和刻蚀孔径的目的,所制备的中间产品24h吸湿率为原材料的2倍,染料吸附量为原材料的1.6倍.     

增塑剂ZS-301的合成研究

以棕榈油为原料合成增塑剂ZS-301，对棕榈油品质(以熔点计)，氢氧化钠用量，氯化锌用量通过正交试验进行了确定。结果表明最佳条件为：选择熔点为48℃～51℃的棕榈油．氢氧化钠的用量16份，氯化锌用量15份(以棕榈油重量为100份计)。在最佳条件下所制得的10批样品均符合国外Pirelli公司同类产品的指标要求。     

极低硫钢的精炼脱硫动力学模型

通过对影响脱硫反应限制性环节的分析,将持续接触和瞬时接触两种反应模式综合考虑,建立了精炼脱硫动力学模型,并通过实验室"极低硫钢精炼脱硫"实验数据对模型进行验证.结果表明,本模型的预测结果与实验结果比较吻合.     

Spectroscopic study on the photophysical properties of chlorine substituted tetraphenylporphyrin-histidine and its zinc(Ⅱ)complexes

As the structural analogues of (bacterio)chlorophylls in photosynthetic organisms, porphyrins play an important role in building up artificial photosynthetic systems[13]. In natural photosynthetic pigment-protein complexes,certain amino acid residuals modulate the reactions ofcharge transfer and energy transfer via coordination andother types of interactions with chlorophyll molecules.For instance, in higher-plant photosynthetic reaction cen-ter PSⅡ, the oxidization-reduction system consistin…     

筒型基础系缆平台沉／拔过程侧摩阻力原型测试

介绍了1999年首次安装在渤海锦州9－3地区多筒基础平台安装施工过程中的侧摩阻力原型测试研究，描述了简型基础上沉和上拔过程中筒壁侧摩阻力的测试系统和测试方法，给出了主要测试结果并与现行规范进行了对比分析，这项成果对筒型基础平台的设计和施工具有直接的参考意义。