Expression of human clotting factor Ⅸ mediated by recom- binant lentiviral vector in cultured cells and hemophilia B mice

To explore the expression of human clotting factor Ⅸ (hFⅨ) cDNA in vitro and the feasibility of gene therapy for hemophilia B mice mediated by recombinant lentiviral vector, a recombinant hFⅨ lentiviral vector driven by ubiquitin-C promoter, FUXW, and by ABP liver specific promoter, FAXW, was constructed respectively. Recombinant lentivirus was harvested from 293T cells by calcium phosphate-mediated transient cotransfection of three plasmids (transgene vector, CMV腞8.2, VSV-G). hFⅨ expression was detected in supernatant of 293T, BHK and L-02 cells infected with FUXW virus, whereas higher expression of hFⅨ levels (630 ng/106 cells/48 h) was detected only in L-02 cells infected with FAXW virus. Serum hFⅨ antigen was detected in all hemophilia B mice treated with FAXW virus by tail vein injection, an efficiency level of hFⅨ was observed (45 ng/mL, approximately 1% of normal human levels), the expression lasted for more than 60 d. The results indicated that HIV-based lentiviral vectors offer a promising approach to the gene therapy of hemophilia B.     

用液膜法从水溶液中提取镍 I.液膜组成及操作条件对分离效果的影响

研究了以兰１１３Ｂ－Ｐ２０４－煤油－Ｈ２ＳＯ４液膜体系从稀ＮｉＳＯ４溶液中分离回收镍的方法。实验结果表明液膜法除Ｎｉ２＋在技术上是可行的。讨论了膜相组成、酸度、乳水比、油内比、搅拌转速及试剂比等因素对传质过程的影响。并考察了膜相的重复使用。     

大豆蛋白质的泡沫分离研究 I.操作工艺条件

在一连续操作的泡沫精馏塔中，考察了各种操作条件对大豆蛋白质溶液泡沫分离过程的影响，包括：进料浓度、进气流量、ｐＨ值、回流比等，确定了较佳的操作条件。用流动法测定了大豆蛋白质在气液界面上的表面过剩浓度并回归了线性吸附方程。结果表明：在溶液浓度较稀时，表面过剩浓度与溶液浓度呈线性关系。     

螺旋压缩机：数学模型和性能计算

本书是施普林格出版社2005年出版的一本关于螺旋压缩机的新著。螺旋式压缩机是一种最常见的用于压缩气体的机器，由于其构造简单、工作效率高而受到人们的关注。本书系统阐述了螺旋压缩机的种类、几何参数转子的设计工作原理和螺旋压缩机的计算。     

浅析市场经济条件下水资源管理的三大转变

在市场经济条件下，水资源管理要实现三大转变：一是由传统防范和利用意识向市场资源型转变，建立起水的资源观、可持续利用观和价值观；二是由行政管水的运行机制向水市场体系转变，逐步建立起市场调节与政府调控相结合的新的管理机制；三是由政出多门的管理体制向水务一体化管理体制转变，建立起“统一、高效、负责”的水务一体化管理体制。     

山西省地表水水功能区划分及其管理

水功能区的划分是实现水资源综合开发、合理利用、积极保护、科学管理的基础工作。根据“全国水功能区划技术细则”，结合对山西省黄河流域的自然、社会情况，尤其是水资源状况的综合分析，系统介绍了水功能区划的方法，对山西省黄河流域河流地表水进行了水功能区划并提出管理建议。     

催化动力学测定痕量钴研究综述

综述了近年来催化动力学光度法测定痕量钴的进展情况。对催化显色动力学光度法、催化褪色动力学光度法、催化荧光光度法、阻抑动力学光度法、催化化学发光光度法等几个类别，从反应介质、灵敏度、线性范围等方面介绍了对不同反应体系的研究情况，并对催化动力学光度法测定痕量钴的发展趋势做出预测。     

RF channel performance assessment for a multiple carrier millimeter-wave communication system

Channel capacity, system versatility and ease of maintenance are factors of prime concern in future generations of wireless communication systems. As a result, millimeter-wave can offer considerable advantages in providing broadband interface. Nonlinearity and noise effects, including parametric fluctuations, are two significant causes for signal degradation in broadband communication systems. Two methods to assess the performance of an RF channel in a multi-carrier system are described. By measuring the forward transfer function from transmitter IF to receiver IF as the frequency is swept across the channel bandwidth, characteristics such as gain flatness, channel dispersion, and saturation effects are revealed. To evaluate the ability of the communication link in preserving the synchronization between two carriers, their heterodyned spectrum at the receiver IF output is studied. Both measurement methods employ standard equipment and have been found to be effective when applied to a multi-carrier millimeter-wave system with over 1 GHz instantaneous bandwidth.     

NPP distribution related to the terrains along the North-South Transect of Eastern China

Vegetation net primary productivity (NPP) is defined as the accumulative amount of organic matters per unit area and time, which is calculated from the difference between accumulative photosynthesis and accumulative autotrophic respiration by green plants. Since the NPP reflects the physiological and ecological characteristics of the plants and the interactive results of the environmental factors, it is an important index to evaluate a structure of ecological system, functional features and …