排序方式: 共有67条查询结果,搜索用时 31 毫秒
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Occupational Health and Safety Systems,Corporate Governance and Viable Systems Diagnosis: An Action Research Approach 总被引:1,自引:1,他引:0
This paper establishes a theoretical basis for the implementation of governance systems in Occupational Health and Safety (OHS). This arose when a complex government organization reviewed the usage of its OHS computer system. This review precipitated several cycles of Action Research (AR). The AR allowed the recognition that the organization needed to define its OHS strategy, clarify its commitment to OHS, and examine its OHS systems in order to provide an effective OHS management and corporate governance system. As part of the review, a Viable Systems Diagnosis was done on the OHS function. It was recognized that Stafford Beer provided valuable insights into the information needs and communication flows of OHS information throughout this complex organization. Action research appears to be a particularly useful method for examining and implementing OHS management systems. 相似文献
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Gemoll T Habermann JK Lahmann J Szymczak S Lundgren C Bündgen NK Jungbluth T Nordström B Becker S Lomnytska MI Bruch HP Ziegler A Hellman U Auer G Roblick UJ Jörnvall H 《Cellular and molecular life sciences : CMLS》2012,69(2):325-333
DNA aneuploidy has been identified as a prognostic factor in the majority of epithelial malignancies. We aimed at identifying ploidy-associated protein expression in endometrial cancer of different prognostic subgroups. Comparison of gel electrophoresis-based protein expression patterns between normal endometrium (n?=?5), diploid (n?=?7), and aneuploid (n?=?7) endometrial carcinoma detected 121 ploidy-associated protein forms, 42 differentially expressed between normal endometrium and diploid endometrioid carcinomas, 37 between diploid and aneuploid endometrioid carcinomas, and 41 between diploid endometrioid and aneuploid uterine papillary serous cancer. Proteins were identified by mass spectrometry and evaluated by Ingenuity Pathway Analysis. Targets were confirmed by liquid chromatography/mass spectrometry. Mass spectrometry identified 41 distinct polypeptides and pathway analysis resulted in high-ranked networks with vimentin and Nf-κB as central nodes. These results identify ploidy-associated protein expression differences that overrule histopathology-associated expression differences and emphasize particular protein networks in genomic stability of endometrial cancer. 相似文献
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Strauss J Zhang Q Verleyen P Huybrechts J Neupert S Predel R Pauwels K Dircksen H 《Cellular and molecular life sciences : CMLS》2011,68(20):3403-3423
We report identification of a beta-type pigment-dispersing hormone (PDH) identical in two water flea species, Daphnia magna and Daphnia pulex. It has been identified by cloning of precursors, chromatographic isolation from tissue extracts followed by immunoassays
and de novo-mass spectrometric sequencing. The peptide is restricted to a complex system of distinct interneurons in the brain
and visual ganglia, but does not occur in neurosecretory cells projecting to neurohemal organs as in decapod crustaceans.
Thirteen neuron types individually identified and reconstructed by immunohistochemistry were almost identical in terms of
positions and projection patterns in both species. Several neurons invade and form plexuses in visual ganglia and major brain
neuropils including the central body. Five neuron types show contralateral pathways and form plexuses in the lateral, dorsal,
or postlateral brain neuropils. Others are local interneurons, and a tritocerebral neuron connects the protocerebrum with
the neuropil of the locomotory second antenna. Two visual ganglia neuron types lateral to the medulla closely resemble insect
medulla lateral circadian clock neurons containing pigment-dispersing factor based upon positional and projectional criteria.
Experiments under 12:12 h light/dark cycles and constant light or darkness conditions showed significant circadian changes
in numbers and activities of one type of medulla lateral PDH neuron with an acrophase in the evening. This simple PDH system
shows striking homologies to PDH systems in decapod crustaceans and well-known clock neurons in several insects, which suggests
evolutionary conservation of an ancient peptidergic interneuronal system that is part of biological clocks. 相似文献
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Hoischen A van Bon BW Rodríguez-Santiago B Gilissen C Vissers LE de Vries P Janssen I van Lier B Hastings R Smithson SF Newbury-Ecob R Kjaergaard S Goodship J McGowan R Bartholdi D Rauch A Peippo M Cobben JM Wieczorek D Gillessen-Kaesbach G Veltman JA Brunner HG de Vries BB 《Nature genetics》2011,43(8):729-731
Bohring-Opitz syndrome is characterized by severe intellectual disability, distinctive facial features and multiple congenital malformations. We sequenced the exomes of three individuals with Bohring-Opitz syndrome and in each identified heterozygous de novo nonsense mutations in ASXL1, which is required for maintenance of both activation and silencing of Hox genes. In total, 7 out of 13 subjects with a Bohring-Opitz phenotype had de novo ASXL1 mutations, suggesting that the syndrome is genetically heterogeneous. 相似文献
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Kaminsky R Ducray P Jung M Clover R Rufener L Bouvier J Weber SS Wenger A Wieland-Berghausen S Goebel T Gauvry N Pautrat F Skripsky T Froelich O Komoin-Oka C Westlund B Sluder A Mäser P 《Nature》2008,452(7184):176-180
Anthelmintic resistance in human and animal pathogenic helminths has been spreading in prevalence and severity to a point where multidrug resistance against the three major classes of anthelmintics--the benzimidazoles, imidazothiazoles and macrocyclic lactones--has become a global phenomenon in gastrointestinal nematodes of farm animals. Hence, there is an urgent need for an anthelmintic with a new mode of action. Here we report the discovery of the amino-acetonitrile derivatives (AADs) as a new chemical class of synthetic anthelmintics and describe the development of drug candidates that are efficacious against various species of livestock-pathogenic nematodes. These drug candidates seem to have a novel mode of action involving a unique, nematode-specific clade of acetylcholine receptor subunits. The AADs are well tolerated and of low toxicity to mammals, and overcome existing resistances to the currently available anthelmintics. 相似文献
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RIM1alpha forms a protein scaffold for regulating neurotransmitter release at the active zone. 总被引:16,自引:0,他引:16
Susanne Schoch Pablo E Castillo Tobias Jo Konark Mukherjee Martin Geppert Yun Wang Frank Schmitz Robert C Malenka Thomas C Südhof 《Nature》2002,415(6869):321-326
Neurotransmitters are released by synaptic vesicle fusion at the active zone. The active zone of a synapse mediates Ca2+-triggered neurotransmitter release, and integrates presynaptic signals in regulating this release. Much is known about the structure of active zones and synaptic vesicles, but the functional relation between their components is poorly understood. Here we show that RIM1alpha, an active zone protein that was identified as a putative effector for the synaptic vesicle protein Rab3A, interacts with several active zone molecules, including Munc13-1 (ref. 6) and alpha-liprins, to form a protein scaffold in the presynaptic nerve terminal. Abolishing the expression of RIM1alpha in mice shows that RIM1alpha is essential for maintaining normal probability of neurotransmitter release, and for regulating release during short-term synaptic plasticity. These data indicate that RIM1alpha has a central function in integrating active zone proteins and synaptic vesicles into a molecular scaffold that controls neurotransmitter release. 相似文献
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