钱谦益唐宋兼宗的祈向与清代诗风新变

“诗必盛唐”是明代具有强势传播力的诗学观念 ,其消极影响是法唐而赝 ,自狭诗道。当时虽有接纳宋诗的呼唤 ,但难以形成趋势。明末清初 ,钱谦益以唐宋兼宗为新的诗学选择 ,具体途辙是以崇尚杜诗为由唐向宋的起点 ,在诗坛大力导入宋代诗风 ,融铸异质 ,求变创新。以沉潜深厚改变浮薄肤浅 ,以性情为本取代唯务格调 ,从而形成宏衍阔大的气局 ,使诗歌创作具有多元组合的美质。钱氏的这一诗学选择开启了清代新诗风     

Identification of a bacterial inhibitor against g-type lysozyme

Lysozymes are antibacterial effectors of the innate immune system in animals that hydrolyze peptidoglycan. Bacteria have evolved protective mechanisms that contribute to lysozyme tolerance such as the production of lysozyme inhibitors, but only inhibitors of chicken (c-) and invertebrate (i-) type lysozyme have been identified. We here report the discovery of a novel Escherichia coli inhibitor specific for goose (g-) type lysozymes, which we designate PliG (periplasmic lysozyme inhibitor of g-type lysozyme). Although it does not inhibit c- or i-type lysozymes, PliG shares a structural sequence motif with the previously described PliI and MliC/PliC lysozyme inhibitor families, suggesting a common ancestry and mode of action. Deletion of pliG increased the sensitivity of E. coli to g-type lysozyme. The existence of inhibitors against all major types of animal lysozyme and their contribution to lysozyme tolerance suggest that lysozyme inhibitors may play a role in bacterial interactions with animal hosts.     

Preparation of superhydrophobic silver nano coatings with feather-like structures by electroless galvanic deposition

Superhydrophobic silver nanocoatings with feather-like morphology are fabricated on copper substrates by electroless galvanic deposition. The coating exhibit superhydrophobicity with a contact angle of 156.4° and glide angle of 4° without any further surface modification. Scanning electron microscope (SEM), X-ray diffraction (XRD) and contact angle measurements are used to investigate the morphology, crystal structure and superhydrophobicity, respectively, of the coatings. The coatings exhibit high thermal stability; their water contact angle did not change when the coatings were heated to 200°C for 2 h. The mechanism of superhydrophobicity of the silver coating is discussed based on the work of Amirfazli, Wenzel and Cassie.     

Molecular basis of parathyroid hormone receptor signaling and trafficking: a family B GPCR paradigm

The parathyroid hormone (PTH) receptor type 1 (PTHR), a G protein-coupled receptor (GPCR), transmits signals to two hormone systems—PTH, endocrine and homeostatic, and PTH-related peptide (PTHrP), paracrine—to regulate different biological processes. PTHR responds to these hormonal stimuli by activating heterotrimeric G proteins, such as G_S that stimulates cAMP production. It was thought that the PTHR, as for all other GPCRs, is only active and signals through G proteins on the cell membrane, and internalizes into a cell to be desensitized and eventually degraded or recycled. Recent studies with cultured cell and animal models reveal a new pathway that involves sustained cAMP signaling from intracellular domains. Not only do these studies challenge the paradigm that cAMP production triggered by activated GPCRs originates exclusively at the cell membrane but they also advance a comprehensive model to account for the functional differences between PTH and PTHrP acting through the same receptor.     

我国旅游管理专业创新人才培养的研究现状分析

为详细研究适应地方需要的高职旅游管理专业创新人才，通过查阅相关的研究文献，分析了当前关于旅游专业创新人才培养目标、人才培养模式、课程建设体系、实践教学体系、教学方法改革等内容，指出了有待于继续研究的高职旅游管理专业创新能力培养的方向与方式问题，为指导高职院校旅游管理专业创新人才培养提供一定的理论借鉴。     

An improved 3-D ray tracing method using linear traveltime interpolation

An approach of three-dimensional seismic ray tracing is presented, which is derived from adopting two-dimensional linear traveltime interpolation (LTI). By adjusting the forward process using the partition of grid interface, and backward step by considering more directions, the new approach is suitable for the application of three-dimensional models. The calculation results show that, with the same accuracy, the improved 3-D method is much faster than the method of traditional LTI directly applied in the three-dimensional case.     

Language clusters based on linguistic complex networks

To investigate the feasibility of using complex networks in the study of linguistic typology, this paper builds and explores 15 lin-guistic complex networks based on the dependency syntactic treebanks of 15 languages. The results show that it is possible to classify human languages by means of the following main parameters of complex networks: (a) average degree of the node, (b) cluster coefficients, (c) average path length, (d) network centralization, (e) diameter, (f) power exponent of degree distribution, and (g) the determination coefficient of power law distributions. The precision of this method is similar to the results achieved by means of modern word order typology. This paper tries to solve two problems of current linguistic typology. First, the language sample of a typological study is not real text; second, typological studies pay too much attention to local language structures in the course of choosing typological parameters. This study performs better in global typological features of language and not only enhances typological methods, but it is also valuable for developing the applications of complex networks in the humanities, social, and life sciences.     

Prediction of sites under adaptive evolution in flavin-containing monooxygenases: Selection pattern revisited

Flavin-containing monooxygenase (FMO), like cytochrome P450 (CYP), is a monooxygenase that uses the reducing equivalents of NADPH to reduce one atom of molecular oxygen to water, while the other atom is used to oxidize the substrate. Recently, it was shown that some CYP isoforms have been subject to positive selection. However, it is unknown whether the highly conserved phase I detoxification enzyme, FMO, has undergone similar positive Darwinian selection. We used maximum-likelihood models of codon substitution, evolutionary fingerprinting, and cross species comparison to investigate the occurrence of adaptive evolution in FMO sequences. We used recent genomic data from a range of species, including vertebrates and invertebrates. We present the evidence for the occurrence of adaptive evolution in mammalian FMO 3, 4, 5, and fugu FMOs but not in mammalian FMO 1, FMO 2, frog FMOs, other fish FMOs and invertebrate FMOs. The sites under adaptive evolution were significantly associated with the insertion domain in mammalian FMO 5. We identified specific amino acid sites in FMOs 3–5 that are likely targets for selection based on the patterns of parallel amino acid change. The most likely role of adaptive evolution is the repair of mutations that permitted optimal NADP+ binding and improved catalytic efficiency. The occurrence of positive selection during the evolution of phase I detoxification enzymes such as FMOs 3–5 and fugu FMO suggests the occurrence of both high selection pressure acting on species within their unique habitats and significant changes in intensity and direction (forms of xenobiotics and drugs) resulting from changes in microhabitat and food.     

Plasmonic biosensors and nanoprobes based on gold nanoshells

Gold nanoshells (GNSs), consisting of a dielectric core coated with gold, have gained extensive attention as they show readily tunable optical properties and good biocompatibility. As highly sensitive and label-free optical biosensors with wide applications, GNSs have been investigated in many fields including drug delivery, immunoassay, cancer treatment, biological sensing and imaging. Taking advantage of the adjustability of the local surface plasmon resonance (LSPR) and the sensitivity of the surfaceenhanced Raman scattering (SERS) signal of GNSs, we have developed diverse applications including plasmonic biosensors and nanoprobes based on GNSs. In this review we introduce plasmonic and electromagnetic properties and fabrication methods of GNSs. We describe research progress in recent years, and highlight several applications of GNSs developed by our group. Finally we provide a brief assessment of future development of GNSs as plasmonic materials that can be integrated with complementary analytical techniques.     

中国现代毒理学的形成与发展

毒理学是关系人类健康的一门学科,人类与毒物斗争的历史是人类文明史中重要的篇章.生物学研究的每一次进展都使得毒理学产生新飞跃,从五四运动至今的90多年内,我国现代毒理学发展经历了起步、取得阶段性成果和开拓创新三个不同的发展阶段.