IFT54 regulates IFT20 stability but is not essential for tubulin transport during ciliogenesis

Intraflagellar transport (IFT) is required for ciliogenesis by ferrying ciliary components using IFT complexes as cargo adaptors. IFT54 is a component of the IFT-B complex and is also associated with cytoplasmic microtubules (MTs). Loss of IFT54 impairs cilia assembly as well as cytoplasmic MT dynamics. The N-terminal calponin homology (CH) domain of IFT54 interacts with tubulins/MTs and has been proposed to transport tubulin during ciliogenesis, whereas the C-terminal coiled-coil (CC) domain binds IFT20. However, the precise function of these domains in vivo is not well understood. We showed that in Chlamydomonas, loss of IFT54 completely blocks ciliogenesis but does not affect spindle formation and proper cell cycle progression, even though IFT54 interacts with mitotic MTs. Interestingly, IFT54 lacking the CH domain allows proper flagellar assembly. The CH domain is required for the association of IFT54 with the axoneme but not with mitotic MTs, and also regulates the flagellar import of IFT54 but not IFT81 and IFT46. The C-terminal CC domain is essential for IFT54 to bind IFT20, and for its recruitment to the basal body and incorporation into IFT complexes. Complete loss of IFT54 or the CC domain destabilizes IFT20. ift54 mutant cells expressing the CC domain alone rescue the stability of IFT20 and form stunted flagella with accumulation of both IFT-A component IFT43 and IFT-B component IFT46, indicating that IFT54 also functions in IFT turn-around at the flagellar tip.     

介质流向对截止阀流场和流阻特性的影响

为探索介质流向对截止阀内部流场和流阻特性的影响,应用SOLIDWORKS软件建立截止阀的三维模型。使用Fluent软件中的有限元法和标准k-ε湍流模型,在不同开度的情况下,采用数值模拟分析的方法,研究不同介质流向下截止阀的流场和流阻特性,分析阀体内部的速度和压力分布规律。结果表明:在两种不同的流向下,阀门的流通能力相差较小;在小开度时,介质高进低出时能够减小阀门的压力损失,在流通高压介质的情况下,高进低出的流向能够极大的减小阀门的关闭力。流场的分析为截止阀的结构设计和优化提供参考。     

铜-石墨复合材料性能与石墨形状和粒径的相关性研究

以电解铜粉、鳞片状石墨粉及不同粒径的近球形石墨粉为原料,通过真空热压烧结工艺制备得到铜-石墨复合材料,并研究石墨形状、粒径对其显微组织、密度、致密度、电导率、硬度及抗压强度等性能的影响;在销盘式摩擦磨损试验机上考察其摩擦磨损性能,通过分析样品磨损表面的形貌,研究石墨形状和粒径对复合材料摩擦磨损性能的影响。结果表明：相比鳞片状石墨粉,采用相同粒径的近球形石墨粉有利于提高复合材料的致密度,获得更优异的力学性能,其抗压强度可以提高近65 MPa;随着近球形石墨粒径从19 μm减小到4 μm,复合材料的致密度、电导率、硬度、抗压强度和摩擦因数均逐渐降低,同时磨损量逐渐增大,其中,复合材料的电导率从28.6 %IACS降低至20.6 %IACS,抗压强度也降低约30 MPa。     

Periodic performance of the chaotic spreadspectrum sequence on finite precision

It is well known that the periodic performance of spread spectrum sequence heavily affects the correlative and secure characteristics of communication systems. The chaotic binary sequence is paid more and more attention since it is one kind of applicable spread spectrum sequences. However, there are unavoidable short cyclic problems for chaotic binary sequences in finite precision. The chaotic binary sequence generating methods are studied first. Then the short cyclic behavior of the chaotic sequences is analyzed in detail, which are generated by quantification approaches with finite word-length. At the same time, a chaotic similar function is defined for presenting the cyclic characteristics of the sequences. Based on these efforts, an improved method with scrambling control for generating chaotic binary sequences is proposed. To quantitatively describe the improvement of periodic performance of the sequences, an orthogonal estimator is also defined. Some simulating results are provided. From the theoretical deduction and the experimental results, it is concluded that the proposed method can effectively increase the period and raise the complexity of the chaotic sequences to some extent.     

Research on the frequency domain ∑△-DPCA

The frequency domain ∑A-DPCA processing （F-∑A-DPCA） is investigated in details and an improved scheme for the F-∑A-DPCA is proposed, which can significantly reduce the computational burden. In practice, because of the sum and difference beam pattern designed independently and other system errors, the clutter suppression of the time domain ∑A-DPCA processing （T-∑A-DPCA） is significantly degraded. However, the F-∑A-DPCA adaptively calculates the optimum gain ratio for motion compensation within each Doppler cells which is robust to system errors. Theoretical analysis and simulation results are presented to validate that the F-∑A-DPCA can achieve superior performance of clutter cancellation than the time domain processing, and its performance can be significantly increased if more pulses are used for the Doppler filtering. The improved approach is efficient, and feasible for real-time application.     

Wigner-Ville distribution and cross Wigner-Ville distribution of noisy signals

The Wigner-Ville distribution （WVD） and the cross Wigner-Ville distribution （XWVD） have been shown to be efficient in the estimation of instantaneous frequency （IF）. But the statistical result of the IF estimation from XWVD peak is much better than using WVD peak. The reason is given from a statistical point of view. Theoretical studies show that XWVD of the analyzed signal can be estimated from XWVD of the noise-contaminated signal. The estimation is unbiased, and the variance is equal to that of noise. In this case, WVD cannot be estimated from W-VD of the noise-contaminated signal. Therefore, higher SNR is required when WVD is used to analyze signals.     

Research on consistency measurement and weight estimation approach of hybrid uncertain comparison matrix

The consistency measurement and weight estimation approach of the hybrid uncertain comparison matrix in the analytic hierarchy process （AHP） are studied. First, the decision-making satisfaction membership function is defined based on the decision making＇s allowable error. Then, the weight model based on the maximal satisfactory consistency idea is suggested, and the consistency index is put forward. Moreover, the weight distributing value model is developed to solve the decision making misleading problem since the multioptimization solutions in the former model. Finally, the weights are ranked based on the possibility degree approach to obtain the ultimate order.     

Control of a key transition from prostrate to erect growth in rice domestication

The transition from the prostrate growth of ancestral wild rice (O. rufipogon Griff.) to the erect growth of Oryza sativa cultivars was one of the most critical events in rice domestication. This evolutionary step importantly improved plant architecture and increased grain yield. Here we find that prostrate growth of wild rice from Yuanjiang County in China is controlled by a semi-dominant gene, PROG1 (PROSTRATE GROWTH 1), on chromosome 7 that encodes a single Cys(2)-His(2) zinc-finger protein. prog1 variants identified in O. sativa disrupt the prog1 function and inactivate prog1 expression, leading to erect growth, greater grain number and higher grain yield in cultivated rice. Sequence comparison shows that 182 varieties of cultivated rice, including 87 indica and 95 japonica cultivars from 17 countries, carry identical mutations in the prog1 coding region that may have become fixed during rice domestication.     

最优资金流施工进度计划的风险分析

本文以合成事件技术为基础,研究进度计划中非关键路径对于关键路的时间干扰问题,定义了安全使用时差的新概念,以便对松驰时间和资金最佳运用情况作出可靠的风险决策。     

航空电子综合化数据传输网络建模方法研究

采用Petri网理论对航空电子综合化数据传输网络系统的建模方法进行研究。通过对研究对象的基本假设、研究对象与Petri网理论之间的关系,给出了航空电子综合化数据传输网络系统的Petri网模型