Ca2+ release induced by inositol 1,4,5-trisphosphate is a steady-state phenomenon controlled by luminal Ca2+ in permeabilized cells.

Low concentrations of inositol 1,4,5-trisphosphate (InsP3) evoke a very rapid mobilization of intracellular Ca2+ stores in many cell types, which can be followed by a further, much slower efflux. Two explanations have been suggested for this biphasic release. The first proposes that the Ca2+ stores vary in their sensitivity to InsP3, and each store releases either its entire contents or nothing (all-or-none release); the second proposes instead that the stores are uniformly sensitive to the effects of InsP3, but that they can release only a fraction of their Ca2+ before their sensitivity is somehow attenuated (steady-state release). Experiments using purified InsP3 receptor molecules reconstituted into lipid vesicles have shown heterogeneity of the receptors in their response to InsP3 under conditions in which the total Ca2+ level at both sides of the receptor is held constant. We now report that in permeabilized A7r5 smooth-muscle cells incubated in Ca(2+)-free medium, the amount of 45Ca2+ remaining in the stores after the rapid transient phase of release is independent of their initial Ca2+ levels, indicating that partially depleted stores are less sensitive to InsP3. Moreover, if the stores are reloaded with 40Ca2+ after the first stimulus, reapplication of the same low concentration of InsP3 will release further 45Ca2+. This recovery of InsP3 sensitivity is almost complete. Under these conditions, Ca2+ release must thus occur by a steady-state mechanism, in which the decreasing Ca2+ content of the stores slows down further release.     

多变量自校正控制计算机辅助设计软件系统

本文介绍了一个可帮助用户进行多变量自校正控制系统设计,而使用户不必了解算法细节的计算机辅助设计(CAD)软件系统;它既包括一些经典的自校正控制算法,又引入了一些近年来新研究的成果。使用情况表明,该系统算法先进,使用方便,控制效果较好,是一个实用的软件工具。     

协商理论简介

本文对协商问题的研究,特别对其与经济理论的联系作了一个简要的说明,重点按照公理形式探讨了协商理论的发展及其成果,并对协商问题在多人情形下的推广和关于协商问题风险性分析等都作了一些介绍。本文还对策略形式探索协商理论的主要发展“轮廓”作了描述。对有关协商理论各分支的研究现状和动态亦作了些评述。     

Exocytotic fusion is activated by Rab3a peptides.

Studies of intracellular traffic in yeast and mammalian systems have implicated members of the Rab family of small GTP-binding proteins as regulators of membrane fusion. We have used the patch clamp technique to measure exocytotic fusion events directly and investigate the role of GTP-binding proteins in regulating exocytosis in mast cells. Intracellular perfusion of mast cells with GTP-gamma S is sufficient to trigger complete exocytotic degranulation in the absence of other intracellular messengers. Here we show that GTP is a potent inhibitor of GTP-gamma S-induced degranulation, indicating that sustained activation of a GTP-binding protein is sufficient for membrane fusion. We have found that synthetic oligopeptides, corresponding to part of the effector domain of Rab3a, stimulate complete exocytotic degranulation, similar to that induced by GTP-gamma S. The response is selective for Rab3a sequence and is strictly dependent on Mg2+ and ATP. This suggests that sustained activation of a Rab3 protein causes exocytotic fusion. The peptide response can be accelerated by GDP-beta S, suggesting that Rab3a peptides compete with endogenous Rab3 proteins for a binding site on a target effector protein, which causes fusion on activation.     

Trying times for human insulin.

The introduction of human insulin to treat diabetics seemed straightforward. What can account for the problems that have followed?     

企业文化定位的影响因素分析

通过讨论企业性质、企业能动要素价值的变迁以及企业的素质和外部环境状况对企业文化定位的影响,认为不同的企业都有其独特的个性,因而企业文化必须根据企业的素质和外部环境的状况以及所处的生命周期阶段来定位,确立其实际的内容.     

分次环的Brown-McCoy根

研究了分次环的Brown-McCoy根,用新的方法证明并推广了文献[1]中的主要结果,证明在比自由群更广泛的群类上分次环的Brown-McCoy根是分次的.