血府逐瘀汤对动脉粥样硬化大鼠细胞间黏附分子和脂质过氧化物的影响

为探讨血府逐瘀汤对动脉粥样硬化(atherosclerosis,AS)大鼠主动脉壁细胞间黏附分子-1(ICAM-1)表达水平和脂质过氧化物的影响,将40只大鼠随机分为正常对照组、模型组、血脂康组、血府逐瘀汤高剂量组、血府逐瘀汤低剂量组,对照组饲喂正常饲料,模型组给予高脂饲料,治疗组在给予高脂饲料的同时给予治疗药物,12周后免疫组化法观察主动脉壁ICAM-1的表达水平、检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)的含量.结果显示:模型组ICAM-1的表达水平呈强阳性表达,治疗组各组均呈弱阳性表达,各组间均有统计学意义(P<0.05).治疗组SOD水平较模型组显著升高,MDA含量显著降低,各组间均有统计学意义(P<0.05).这说明血府逐瘀汤可以通过下调ICAM-1的表达,清除脂质过氧化物、保护内皮细胞起到抗AS的作用.     

学术论文结束语的撰写要求探讨

学术论文的结束语,对吸引读者深入阅读全文发挥着重要作用。从内容要求、语言要求、结论和结语的区别等方面阐述了学术论文结束语的写作要求,对常见问题进行了分析并给出了建议。指出结束语是读者获得新知识、新经验的途径之一,在实践中应重视其写作。     

高校图书馆提高教学与科研服务水平探讨

阐述了高校图书馆为教学与科研服务的现状,分析了我国高校图书馆为教学与科研服务中存在的问题,提出了高校图书馆提高教学与科研服务能力的方法及建议。     

The SH3-binding domain of Cx43 participates in loop/tail interactions critical for Cx43-hemichannel activity

Connexin 43 (Cx43) hemichannels establish local signaling networks via the release of ATP and other molecules, but their excessive opening may result in cell death. Hence, the activity of Cx43-hemichannels ought to be critically controlled. This involves interactions between the C-terminal tail (CT) and the cytoplasmic loop (CL), more particularly the L2 domain within CL. Previous work revealed an important role for the last nine amino acids of the Cx43 CT by targeting the L2 domain, as these nine amino acids were sufficient to restore the activity of CT-truncated Cx43-hemichannels. However, we discovered that deletion of the last 19 amino acids of the CT only partially lowered the binding to the L2 domain, indicating that a second L2-binding region is present in the CT. We here provide evidence that the SH3-binding domain is another CT region that targets the L2 domain. At the functional level, the SH3-binding domain was able to restore the activity of CT-truncated Cx43-hemichannels and alleviate the inhibition of full-length Cx43-hemichannels by high intracellular Ca²⁺ concentration ([Ca²⁺]_i) as demonstrated by various approaches including patch clamp studies of unitary Cx43-hemichannel activity. Finally, we show that in full-length Cx43-hemichannels, deletion of either the SH3-binding domain or the CT9 region suppresses the hemichannel activity, while deletion of both domains completely annihilates the hemichannel activity. These results demonstrate that the Cx43 SH3-binding domain, in addition to the CT9 region, critically controls hemichannel activity at high [Ca²⁺]_i, which may be involved in pathological hemichannel opening.     

WNK signalling pathways in blood pressure regulation

Hypertension (high blood pressure) is a major public health problem affecting more than a billion people worldwide with complications, including stroke, heart failure and kidney failure. The regulation of blood pressure is multifactorial reflecting genetic susceptibility, in utero environment and external factors such as obesity and salt intake. In keeping with Arthur Guyton’s hypothesis, the kidney plays a key role in blood pressure control and data from clinical studies; physiology and genetics have shown that hypertension is driven a failure of the kidney to excrete excess salt at normal levels of blood pressure. There is a number of rare Mendelian blood pressure syndromes, which have shed light on the molecular mechanisms involved in dysregulated ion transport in the distal kidney. One in particular is Familial hyperkalemic hypertension (FHHt), an autosomal dominant monogenic form of hypertension characterised by high blood pressure, hyperkalemia, hyperchloremic metabolic acidosis, and hypercalciuria. The clinical signs of FHHt are treated by low doses of thiazide diuretic, and it mirrors Gitelman syndrome which features the inverse phenotype of hypotension, hypokalemic metabolic alkalosis, and hypocalciuria. Gitelman syndrome is caused by loss of function mutations in the thiazide-sensitive Na/Cl cotransporter (NCC); however, FHHt patients do not have mutations in the SCL12A3 locus encoding NCC. Instead, mutations have been identified in genes that have revealed a key signalling pathway that regulates NCC and several other key transporters and ion channels in the kidney that are critical for BP regulation. This is the WNK kinase signalling pathway that is the subject of this review.     

高校图书馆特藏资源建设研究

探讨了高校图书馆特藏资源建设的现状,阐述了高校图书馆特藏资源建设的不足,指出了高校图书馆特藏资源建设的方向,提出了提升高校图书馆特藏资源服务水平的对策。     

NBR_0代数上的运算和性质

通过提出模糊逻辑代数NBR_0的概念,研究逻辑代数NBR_0的代数结构,定义其上的运算,并探讨新运算,→_1,→_2与原运算之间关系,得到了NBR_0代数的一个等价刻画.     

关于 heparosan 中文译名的建议

heparosan 是某些细菌荚膜中多糖骨架的二糖重复单位,也是肝素和硫酸乙酰肝素的生物合成前体。目前医学界以 heparosan 为起始物,通过化学或酶修饰已获得一系列的类似物。因此,heparosan 亟需一个更加准确的、能够揭示其内涵的中文名。根据 heparosan 在肝素类多糖生物合成中的作用以及术语翻译的基本命名规则,建议将 heparosan 的中文译名定为“肝素原”。     

高校异构系统数据整合的设计与实现

近年来，为提高工作效率，简化业务流程，我国高校开发了多种与自身业务相关的应用系统。随着校内各种应用系统的增多，为避免“信息孤岛”的出现，学校对不同业务系统中的数据信息实现整合的愿望，也越来越迫切。基于此，文章介绍了高校数据整合应用系统在设计与实现中主要的研究内容和实施难点。分析了NOVELL Identity Manager 3关键技术，并根据实际工作经验，对系统的架构作出了比较详细的说明。