测定血小板衍生生长因子活性方法的改进

以BALB／C小鼠胚胎成纤维细胞(BALB／C3T3)作为靶细胞，经20mmol／L醋酸钠平衡后的CM—Sepharose(CMS)吸附过的胎牛血清作为基础培养血清，应用[3-(4，5-dimethylthi—azolyl)-2，5-diphenyhetrazolium bromide，MTT]方法测定血小板源生长因子(platelet—derived growth factor，PDGF)诱导细胞增殖的生物活性，结果表明，该方法具有良好的量效关系，且精确度、重现性均较高。     

科技孵化企业聚集知识溢出效应的理论分析

阐述了科技孵化企业知识溢出效应的内涵与机理．在企业知识分类的基础上，运用新经济增长理论的知识溢出理论建立技术企业聚集的生产函数，并分别对不同技术领域企业、同技术领域非竞争企业和同技术领域竞争企业3种类别科技孵化企业聚集的知识溢出效应进行了理论分析和推导．结果表明，同技术领域非竞争企业聚集更有效率，从理论上证明了相对宽泛的专业孵化器更有效的命题，为企业孵化器的建设并深化其服务提供了理论依据．     

转变教育思想教育观念是地方高校的首要问题

教育思想、教育观念是高等教育改革和发展的灵魂.一般地方高校在教育思想、教育观念上对教学中心地位,教学和科研的关系,素质教育,教育管理以及办学的定位、办学思路等方面容易产生一些认识上的误区.打破旧观念,树立"理论知识传授与实践能力培养并重","协调发展","综合素质教育和人的全面发展","多元质量","以人为本,人才强校"的新观念是地方高校在改革发展中应解决的首要问题.     

NPP distribution related to the terrains along the North-South Transect of Eastern China

Vegetation net primary productivity (NPP) is defined as the accumulative amount of organic matters per unit area and time, which is calculated from the difference between accumulative photosynthesis and accumulative autotrophic respiration by green plants. Since the NPP reflects the physiological and ecological characteristics of the plants and the interactive results of the environmental factors, it is an important index to evaluate a structure of ecological system, functional features and …     

RNA interference-mediated inhibition of Hepatitis B Virus replication

Persistent and recurrent infection of hepatitis B virus (HBV) represents one of the most common and severe viral infections of humans, and has caused a formidable health problem in the affected countries. Currently used antiviral drugs have a very limited success on controlling HBV replication and infection. RNA interference (RNAi), a process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of target mRNA in mammalian and plant cells, has recently been used to knockdown gene expression in various species. In this study, we sought to determine whether RNAi-mediated silencing of HBV viral gene expression could lead to the effective inhibition of HBV replication. We first developed RNAi vectors that expressed small interfering RNA (siRNA) and targeted the HBV core or surface gene sequence. Our results demonstrated that these specific siRNAs efficiently reduced the levels of corresponding viral RNAs and proteins, and thus suppressed viral replication. Treatment with siRNA gave the greatest reduction in the levels of HBsAg (92%) and in HBeAg (85%) respectively in the cultured cell medium. Our findings further demonstrated that the RNAi-mediated antiviral effect was sequence-specific and dose-dependent. Therefore, our findings strongly suggest that RNAi-mediated silencing of HBV viral genes could effectively inhibit the replication of HBV, hence RNAi-based strategy should be further explored as a more efficacious antiviral therapy of HBV infection.     

JANSIM:基于Java的主动网络仿真模块

原有的主动网络仿真模块对于现有主动网系统的支持度较低，使得开发成本和学习成本都很高。基于Java的主动网络仿真模块JANSIM，实现了在ANTS上开发的主动协议可以无需重新在仿真环境下重新编写即可利用仿真环境的网络仿真功能对其进行仿真测试，从而大幅度的降低了主动网协议开发者的学习成本和移植成本。JANSIM包括ANTS-ESIB的适配嚣包、ANTS协议转换器和ANTS脚本转换器。ANTS-ESIB适配器可以使得基于JANSIM新开发的ANTS应用直接运行在仿真系统上，两个转换器则可以使得原先开发的ANTS协议和脚本自动转换为基于JANSIM的协议，从而在仿真环境下运行。     

A HEURISTIC PATH-PLANNING METHOD FOR ENHANCING MACHINE-TOOL CONTOUR-FOLLOWING

Nomenclature ki integral action coefficient[s-1]kp speed proportional gain[s-1]kv position proportional gain[s-1]kf feed forward factor of the speed loop[%]M axis mass[kg]V speed[m/s]F driving effort[N]Rc radius of curvature[m]d distance to the apex[m]o lateral offset[m]εcontouring error[m]ωm lowest mechanical resonant frequency[s-1]ζdamping coefficient[-]r inertia ratio[-]μmembership function[-]αcorner angle[rad]x,y horizontal and vertical coordinates[m]?angle to the normal line[rad]1.I…     

MERGING OPTIMALITY CONDITIONS WITH GENETIC ALGORITHM OPERATORS TO SOLVE SINGLE MACHINE TOTAL WEIGHTED TARDINESS PROBLEM

1.Introduction Production sequencing and scheduling is one of the most important activities in production planning and control.Sequencing is defined as the order in which the jobs are processed through the machines.The allocation of machines over time to process a collection of jobs is defined as Scheduling.Several methods have been developed to solve the scheduling problem which can be classified as follows:1)efficient optimal methods,2)implicit and explicit,or complete enumerative methods,a…     

单片机仿真实验的探讨与研究

本文介绍了传统的单片机实验方法和利用Proteus（http：／／www．labcenter.co．uk）软件进行单片机仿真实验。包括从元器件的选择、电路的连结、源程序的输入及调试、仿真结果的输出，并比较了各自的特点。     

Development of the CAS-IBB single-particle microbeam for localized irradiation of living cells

A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydrogen ions, produced by a van de Graaff accelerator, in an energy range of 2.0-3.0 MeV, into an area smaller than that of the nucleus of an individual living cell. The beam is collimated by a borosilicate glass capillary that forms the beam-line exit. An integrated computer program recognizes the cells and locates them one by one over the microbeam exit for irradiation. We present technical details of the CAS-LIBB microbeam facility, particularly on the collimator, hardware, control program, as well as cell irradiation protocols available. Various factors contributing to the targeting and positioning precision are discussed along with accuracy measurement results.