Energetic landscape of alpha-lytic protease optimizes longevity through kinetic stability.

During the evolution of proteins the pressure to optimize biological activity is moderated by a need for efficient folding. For most proteins, this is accomplished through spontaneous folding to a thermodynamically stable and active native state. However, in the extracellular bacterial alpha-lytic protease (alphaLP) these two processes have become decoupled. The native state of alphaLP is thermodynamically unstable, and when denatured, requires millennia (t1/2 approximately 1,800 years) to refold. Folding is made possible by an attached folding catalyst, the pro-region, which is degraded on completion of folding, leaving alphaLP trapped in its native state by a large kinetic unfolding barrier (t1/2 approximately 1.2 years). alphaLP faces two very different folding landscapes: one in the presence of the pro-region controlling folding, and one in its absence restricting unfolding. Here we demonstrate that this separation of folding and unfolding pathways has removed constraints placed on the folding of thermodynamically stable proteins, and allowed the evolution of a native state having markedly reduced dynamic fluctuations. This, in turn, has led to a significant extension of the functional lifetime of alphaLP by the optimal suppression of proteolytic sensitivity.     

Comparative study of the karyotypes of two Egyptian species of bats,Taphozous perforatus andTaphozous nudiventris (Chiroptera: Mammalia)

A detailed karyotypic analysis of two Egyptian species of bats,Taphozous perforatus andTaphozous nudiventris, was made on the basis of conventional data and G-band patterns. No detectable karyotypic difference was found between the two species (2n=42, F.N. 64). By comparison of G-band patterns, some differences between the two species were seen in the corresponding autosomal pairs. These results are reported for the first time in Egypt.     

对模糊随机向量凸集的定义及探讨

在模糊随机变量的基础上引入了模糊随机向量凸集的概念,给出了几个模糊随机凸集的例子.     

SIMULTANEOUS REACTION AND LIQUID-LIQUID EXTRACTION IN THE HYDROGEN PEROXIDE PRODUCTION

The gas-liquid-liquid reactive extraction system for preparing hydrogen peroxide via anthraquinone was investigated. The oxidation reaction of hydrogenated working solution was combined with the extraction of hydrogen peroxide from working solution in a sieve plate column. The reaction of 2-ethylanthrahydroquionone with oxygen and the liquid-liquid extraction of hydrogen peroxide take place simultaneously. The oxygen was introduced with hydrogenated working solution through a nozzle in the bottom of the column, which worked as agitated air as well as oxidation reagent. The results showed the oxidation and extraction do not hamper each other, on the contrary, the presence of oxidation gas in the column can promote the transfer of hydrogen peroxide from organic phase to aqueous phase, thus the reaction efficiency and extraction efficiency increased with increasing gas superficial velocity. Furthermore, the oxidation efficiency is almost 100% and the extraction efficiency is higher than 90% in this process.     

Detecting myocardial ischemia with 2-D CVIB imaging method--an in vivo animal experiment study

A 2-D cyclic variation of integrated backscatter (CVIB) imaging method was established for detecting myocardial ischemia. To demonstrate the feasibility and validity of this method, animal experiments were conducted. Acute myocardial ischemia was induced by occluding left anterior descending coronary artery in 10 anesthetized open-chest dogs. While scanning the normal hearts and the ischemic hearts with a B scanner, digital radiofrequency data were acquired by a real-time acquisition system in synchronism. The offline analysis to the radio-frequency signal with the 2-D CVIB imaging method was performed to verify the consistency between the imaging result and the design of the experiment. In addition, 4 dogs in experiment were treated with the heart pacemaker in order to investigate the influence of changing in heart rate on the detection of ischemic myocardium with the proposed method. The experimental result showed that the 2-D CVIB imaging method succeeded in detecting the ischemic myocardium and is a new non-invasive way for the cardiologists to both quantitatively and visually evaluate the contractile performance of the myocardium.     

IT系统在企业中的应用

从实际应用的角度，阐述了IT系统在企业中的应用和建设问题。从面向客户的角度出发，对企业IT系统进行了划分，并讨论了各系统之间逻辑关系及其具体功能，在此基础上，对各系统的具体建设内容进行了重点分析。     

高温多雨地区高等级沥青路面材料的分析与应用措施

针对南方高温多雨地区的气候特点 ,为解决沥青混凝土路面修筑后的高温稳定性和水稳定性这两个突出的问题 ,依据本单位的一个工程实践 ,从集料、沥青、级配设计、施工工艺等方面进行了系统的分析与阐述。说明高温多雨地区高等级沥青路面只要材料科学选择、严格控制、应用措施得当 ,质量是能够达到规范要求的     

一种评定实验教学质量与学习效果的方法研究

本文分析了实验教学评价的特殊性、复杂性与重要性。介绍了运用权重法评定实验教学效果的基本步骤方法。设计了用权重法评定实验教学质量与学习效果的两例评估计算方法。提出了还可以根据实验教学的实践性特点,用权重法设定专项评价和制作自动评价系统课件的设想。     

基于IP多播的机房信息管理系统的设计与实现

提出了一种基于IP多播技术的解决方案并加以实现,满足了对上机学生按系或班级分组进行管理与各类统计的需要,实现了传统C/S结构所能实现的全部功能,解决了传统机房信息管理系统使用带来的问题。