Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin

When used in combination with certain chemotherapies, curcumin has been shown to increase apoptosis in several cancer cell lines. Here, we report the combined effects of curcumin and cinobufacini on human cervical carcinoma cells. The aim of this study was to examine whether curcumin could enhance apoptosis induced by cinobufacini. 3-(4,5-Dimethylthiazol-2-y1)-2,5- diphenytetrazolium bromide (MTT) assays revealed that the growth and proliferation of HeLa cells could be inhibited by 75% after a combined treatment of 25 μg/mL cinobufacini and 8 μg/mL curcumin. The combined treatment is 3 times more effective than treatment with 25 μg/mL cinobufacini alone. Annexin V-FITC/PI staining, morphological changes and immunofluorescence verified a significant enhancement in cinobufacini-induced apoptosis when cells were also exposed to curcumin. The data showed that the proportion of early apoptotic cells significantly increased from 15.43% in cells treated only with 25 μg/mL cinobufacini to 49.2% in cells treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. Moreover, compared with treatment of only 25 μg/mL cinobufacini, ROS production increased 1.7-fold, the intracellular free Ca 2+ concentration increased 1.5-fold, and the mitochon- drial membrane potential decreased by 20% in the combined treatment. Simultaneously, the atomic force microscopy (AFM) re- sults suggest that cells treated with a combination of cinobufacini and curcumin varied significantly in shape and ultrastructure. Collapsed cells with leaking cytoplasm, blebbing pores and emerging apoptotic bodies were prevalent. The nanoparticle size increased from 70 nm when the cells were treated with 25 μg/mL cinobufacini to 190 nm when the cells were treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. The size increase resulted in the cell membrane becoming considerably rough. These results can improve our understanding of combination treatments. Specifically, the combination of cinobufacini and curcumin may potentially find use as a novel cervical carcinoma treatment. Additionally, AFM is a powerful tool that can be used to explore cellular morphologies and ultrastructures.     

Ground demonstration system based on in-orbit assembly oriented manipulator flexible force control

To eliminate the load weight limit of carrier rockets and reduce the burden on support struc-tures,in-orbit assembly is a key technology to make design of scattering a large diameter telescope into submirror modules, which requires smooth operation of assembly robots, and flexible force con-trol technology is necessary.A ground demonstration system is presented for in-orbit assembly focu-sing on flexible force control.A six-dimensional force/torque sensor and its data acquisition system are used to compensate for gravity.For translation and rotation, an algorithm for flexible control is proposed.A ground transportation demonstration verifies accuracy and smoothness of flexible force control, and the transportation and assembly task is completed automatically.The proposed system is suitable for the development of in-orbit assembly robots.     

基于TOPSIS法的土地利用与区域发展协调性评价——以安徽省宿州市为例

利用宿州市2000～2010年土地利用变更调查数据与社会经济发展数据,构建了土地利用与区域发展协调性评价指标体系,采用TOPSIS方法对土地利用与区域发展的协调性进行了综合评价,并采用相关分析法,对评价指标体系中的各指标与协调性的相关程度进行了分析.结果表明:2000～2010年宿州市土地利用与区域发展之间的协调性不断优化;2000年协调度最低,其值为0.287,2010年协调度最高,其值为0.732;2000～2004年期间协调性缓慢上升、略有反复,2004～2008年呈加速上升态势,2008～2010年为协调性高速上升阶段;土地利用变化导致了社会经济的正向变化,对宿州市社会经济发展起到了正向的推动作用.宿州市土地利用与区域发展之间协调性逐年上升主要是因为宿州市区域发展战略的调整、经济增长方式的转变以及土地管理制度的改进等因素造成的.     

The mammalian sodium channel BNC1 is required for normal touch sensation

Of the vertebrate senses, touch is the least understood at the molecular level The ion channels that form the core of the mechanosensory complex and confer touch sensitivity remain unknown. However, the similarity of the brain sodium channel 1 (BNC1) to nematode proteins involved in mechanotransduction indicated that it might be a part of such a mechanosensor. Here we show that disrupting the mouse BNC1 gene markedly reduces the sensitivity of a specific component of mechanosensation: low-threshold rapidly adapting mechanoreceptors. In rodent hairy skin these mechanoreceptors are excited by hair movement. Consistent with this function, we found BNC1 in the lanceolate nerve endings that lie adjacent to and surround the hair follicle. Although BNC1 has been proposed to have a role in pH sensing, the acid-evoked current in cultured sensory neurons and the response of acid-stimulated nociceptors were normal in BNC1 null mice. These data identify the BNC1 channel as essential for the normal detection of light touch and indicate that BNC1 may be a central component of a mechanosensory complex.     

生物质谱在生命科学中的应用

生物质谱因其高灵敏度、高准确度、快速、易于自动化等特点，使其在核酸化学、蛋白质组学、糖生物学、细胞信号转导等生命科学领域得到了广泛应用．随着生物质谱在生物研究领域的普及和仪器自动化程度的不断提高，该技术的发展将会更加迅速。     

乳腺癌PKCs表达与其分化程度的相关性

为了研究蛋白激酶C（PKC）和乳腺癌分化及转移的相关性，采用免疫组化SABC方法检测48例人乳腺癌的PKC蛋白表达，包括PKCα、βI、η和ξ等蛋白。结果表明，与分化低的乳腺癌对比，分化高的乳腺癌PKC蛋白表达水平提高。在临床I－II和III级的乳腺癌中，PKCη蛋白表达率分别为61．5％和9．1％，具有显著意义（P＜0．05），PKCξ蛋白表达率分别为92．3％和27．3％，具有极显著意义（P＜0．01）。另外，在乳腺癌未转移的病例中，PKCs蛋白表达水平普遍较高，其中PKCξ与肿转移相关性极显著（P＜0．01）。此外，PKCs蛋白表达水平与肿瘤患者年龄无关。以上结果提示，PKCη和PKCξ有可能作为评价临床乳腺癌分化和转移程度的指标之一。     

阿尔茨海默病动物模型和治疗的相关进展

1906年11月3日,在德国Tübingen的一次会议上,巴伐利亚精神病学家Alois Alzheimer首次描述了随后以其名字命名的一种神经退行性综合症的病理特征,即阿尔茨海默症(Alzheimer's disease,AD),亦称老年痴呆[1].     

利用夹点技术对精馏系统换热网络的调优分析

利用夹点技术对永坪炼油厂100万吨／年常压蒸馏装置换热网络进行了调优分析，找出了节能方面存在的问题，为进一步采取节能措施和途径提供了依据。     

水平井多封隔器管柱力学分析及安全评价

针对水平井多封隔器管柱在井下多种载荷的共同作用下易引起管柱失效的安全问题,基于力学研究中的多杆件的多次超静定结构,引入力法对多封隔器之间管柱进行受力分析,同时考虑温度效应、活塞效应、鼓胀效应和螺旋屈曲效应的影响,建立考虑多封隔器影响的轴向力数学模型;在此基础上建立多封隔器管柱力学安全强度评价准则和封隔器控制信封失效判断方法,对现场水平井多封隔器管柱进行力学分析和安全评价。研究结果表明,建立的考虑多封隔器影响的轴向力数学模型计算结果大于常规不考虑多封隔器影响时轴向力,若不考虑多封隔器的影响将低估多封隔器管柱的轴向力,而造成管柱受力危险;同时对管柱危险位置和封隔器进行安全评价,得到了酸化作业时油压和套压的综合控制参数,以期为水平井多封隔器管柱安全工作参数的优化设计提供理论依据。     

渤海湾盆地新生代构造演化

渤海湾新生代盆地经历了早第三纪裂陷沉降和晚期第三纪－第四纪后裂陷热沉降两个阶段。基本完成了一个完整的裂陷作用旋回。早第三纪裂陷作用包括三个裂陷伸展期，它们分别相当于孔店级－沙四段、沙三段、沙二段－沙一段－东营组等三套地层的发育时期，各裂陷伸展期的构造运动学特征有一定的差异，反映在伸展构造样式的变化上，大致可归纳为由多米诺式正断层系统向铲式正断层系统、坡坪式正断层系统的演化序列及由非旋转平面式正断层系统向铲式正断层系统、坡坪式正断层系统的演化序列两个演化模式。