Genome editing and animal models

Transgenic technology allows a gene of interest to be introduced into the genome of a laboratory animal, and provides an extremely powerful tool to dissect the molecular mechanisms of disease. Transgenic mouse models made by microinjection of DNA into zygotic pro- nuclei in particular have been widely used by the genetics community for 30 years. However, it remains a rather crude approach： injected sequences randomly insert in multiple copies as concatamers, they can be mutagenic, and they have variable or silenced expression depending on the site of integration, a phenomenon called position effects. As a result, multiple lines are required in order to confirm appropriate transgene expression. This can be partially overcome by flanking transgenes with insulator sequences to protect the transgene from the influence of the sur- rounding regulatory elements. Large （〈300 kb） BAC- based transgenic vectors have also been shown to be more resistant to position effects. However, animals carrying extra copies of fairly large regions of the genome could have unpredictable phenotypes. The most effective method used to control for position effects is to target transgene insertion to specific genomic loci, the so-called targeted transgenesis; for instance, the fast, site-specific transgenic technology TargattTM. The purpose of this review is to provide an overview on the current existing methods for making targeted transgenic mouse models.     

英语核心词汇研究综述

核心词汇是最简单、最基本的词汇,在二语习得过程中占有重要地位,同时在日常交际中也举足轻重。回顾并总结核心词汇方面的相关研究,对核心词汇的概念、特征和界定方法做详细阐述,可以发现,核心词汇在词汇学习中占有重要地位,对外语教学及词典编纂具有重要意义。     

黔东南施秉白云岩喀斯特地区生物多样性与世界遗产价值

通过对中国南方喀斯特第二批提名预选地贵州施秉白云岩喀斯特地区28 295 ha范围内的生物物种进行初步调查,发现该地区物种资源丰富,共有高等植物1 352种。其中,列入中国物种红色名录的高等植物有70种,列入IUCN红色名录的有16种,列入濒危野生动植物国际贸易公约(CITES)的有26种。共有脊椎动物298种,还有丰富的昆虫和洞穴动物。其中,列入中国物种红色名录的脊椎动物有243种,列入IUCN红色名录的有21种,列入濒危野生动植物国际贸易公约(CITES)的有41种。该研究区的区系具有古老残遗的特性,植物区系是温带与热带亚热带区系并重,而陆生脊椎动物的区系则表现了亚热带特点。据初步统计,研究区的植被类型有针叶林、针阔混交林、常绿阔叶林、常绿落叶阔叶混交林、竹林、灌丛等8个森林植被型组、61个群系;根据动物适应白云岩喀斯特环境的特点,大致将研究区内的动物群落划分为溪河动物群、河谷芦竹与河滩灌丛动物群、森林动物群、竹林动物群、岩岸动物群、灌丛灌草丛动物群、农田村寨动物群和洞穴动物群等群落类型。施秉喀斯特提名预选地初步反映了陆地白云岩喀斯特生态系统和动植物群落正在进行的重要的生态和生物演化过程,包含了就地保护生物多样性最重要和显著的自然栖息地,包括从科学和保护的角度看具突出普遍价值的濒危物种的栖息地,基本满足世界自然遗产(ix)和(x)条评价标准。     

《初等代数研究》课程教学改革初探

从高师数学教育培养目标和《初等代数研究》课程的教学特点出发，分析了目前教学过程中存在的问题，探讨本课程的教育功能和在教学实践中所采取的部分措施．     

Fractal characteristics of round jets in steady crossflow

The fractal dimensions of turbulent round jets in steady crossflow has been analyzed by using planar laser-induced fluorescence (PLIF) method. The relation between the fractal dimension and the momentum ratio, the variation of the fractal dimension with the elevation of jet and the dilution have been investigated. The comparison of the fractal characteristics between the multiple jets and the signal jet has been carried out.     

Microelectronics-embedded channel bridging and signal regeneration of injured spinal cords

Due to the difficulty in spinal cord regeneration with biological methods, the microelectronic neural bridge, a new concept based on microelectronic technology, is presented. The microelectronic system has been realized in the forms of hybrid and integrated circuits. The integrated circuits for neural signal detection, stimulation, and regeneration are realized in a CMOS process. In animal experiments with 100 toads, 48 rats, and 3 rabbits, nerve signals have been successfully detected from spinal cords and sciatic nerves, and functional electrical stimulation has been carried out for spinal cords and sciatic nerves. When the microelectronic system is bridged between the controlling and stimulated nerve, the relevant motion of legs and nerve signal waveforms, which are stimulated by the evoked or spontaneous nerve signal through such a system, have been observed. Therefore, the feasibility of the presented method was demonstrated.     

Preparation and kinetic analysis of Li4SiO4 sorbents with different silicon sources for high temperature CO2 capture

Using diatomite and analytical pure SiO2 as silicon sources,Li4SiO4 sorbents for high temperature CO2 capture were prepared through solid-state reaction method.Phase composition was analyzed by X-ray diffraction,and the CO2 absorption capacity and absorptoin-desorption performance were studied by the simultaneous thermal thermogravimetric analyzer(TG-DSC).The results showed that silicon source had an important influence on CO2 absorption properties.The kinetic parameters for the chemisorption and diffusion processes were obtained by the isothermal study for different silicon sources.The results showed that the activation energies for these two processes were estimated to be 105.427 and 35.928 kJ/mol for the sample with analytical pure SiO2(AS).While for the sample with diatomite(DS),the activation energies for these two processes were estimated to be 78.500 and 20.439 kJ/mol,respectively.     

加强数字化校园建设促进学校精细化管理

学校精细化管理是提升学校内涵的关键.随着现代信息技术的高速发展和广泛使用,为精细化管理提供了有力的技术手段和支撑平台,高校要以数字化校园建设为契机,实现精细化与数字化的同步运行,最终实现学校管理的全面信息化.     

悬垂美感与织物结构特性的关系讨论

通过多元逐步回归，讨论了织物悬垂美感指标与织物结构特性指标间的内在联系，揭示了二者密切相关的特性．     

恒电位脉冲法检测高纯铁素体不锈钢的敏化

用恒电位脉冲（ＰＰＴ）法对高纯铁素体不锈钢的敏化度进行了检测，并与传统的动电位再活化（ＥＰＲ）法进行了比较，结果表明：ＰＰＴ法可以用来检测铁素体不锈钢的敏化，并且具有检测速度快，表面损伤小的优点