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21.
Schwarz-Linek U Werner JM Pickford AR Gurusiddappa S Kim JH Pilka ES Briggs JA Gough TS Höök M Campbell ID Potts JR 《Nature》2003,423(6936):177-181
Staphylococcus aureus and Streptococcus pyogenes, two important human pathogens, target host fibronectin (Fn) in their adhesion to and invasion of host cells. Fibronectin-binding proteins (FnBPs), anchored in the bacterial cell wall, have multiple Fn-binding repeats in an unfolded region of the protein. The bacterium-binding site in the amino-terminal domain (1-5F1) of Fn contains five sequential Fn type 1 (F1) modules. Here we show the structure of a streptococcal (S. dysgalactiae) FnBP peptide (B3) in complex with the module pair 1F12F1. This identifies 1F1- and 2F1-binding motifs in B3 that form additional antiparallel beta-strands on sequential F1 modules-the first example of a tandem beta-zipper. Sequence analyses of larger regions of FnBPs from S. pyogenes and S. aureus reveal a repeating pattern of F1-binding motifs that match the pattern of F1 modules in 1-5F1 of Fn. In the process of Fn-mediated invasion of host cells, therefore, the bacterial proteins seem to exploit the modular structure of Fn by forming extended tandem beta-zippers. This work is a vital step forward in explaining the full mechanism of the integrin-dependent FnBP-mediated invasion of host cells. 相似文献
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多孔泡沫材料的声吸收特性 总被引:5,自引:4,他引:5
为了结合泡沫金属兼有的高吸声和高热传导两种特性以进一步提高其吸声性能,回顾了泡沫金属材料的应用和声学建模;通过对泡沫金属和用于制造泡沫金属的高分子基体材料的实验,比较了作者提出的声波通过泡沫金属传播的3种黏滞模型的预测结果,表明所有模型在泡沫金属典型胞元尺寸所对应的低雷诺数范围内是有效的(假定声波为线性,幅值低于160 dB).第一种模型考虑了声波沿平行于刚性圆柱束轴线方向传播时所受到的空气曳力,第二种模型考虑了声波沿垂直于刚性圆柱束轴线方向的传播,第三种模型考虑了声波通过球形节点的传播.结合这3种模型,提出了一种泡沫金属声学性能的综合模型,可以用来预测泡沫金属的声吸收特性.此外,还介绍了一种用于泡沫金属材料基本声传播特性实验的后处理技术. 相似文献
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Loftus B Anderson I Davies R Alsmark UC Samuelson J Amedeo P Roncaglia P Berriman M Hirt RP Mann BJ Nozaki T Suh B Pop M Duchene M Ackers J Tannich E Leippe M Hofer M Bruchhaus I Willhoeft U Bhattacharya A Chillingworth T Churcher C Hance Z Harris B Harris D Jagels K Moule S Mungall K Ormond D Squares R Whitehead S Quail MA Rabbinowitsch E Norbertczak H Price C Wang Z Guillén N Gilchrist C Stroup SE Bhattacharya S Lohia A Foster PG Sicheritz-Ponten T Weber C Singh U Mukherjee C El-Sayed NM 《Nature》2005,433(7028):865-868
Entamoeba histolytica is an intestinal parasite and the causative agent of amoebiasis, which is a significant source of morbidity and mortality in developing countries. Here we present the genome of E. histolytica, which reveals a variety of metabolic adaptations shared with two other amitochondrial protist pathogens: Giardia lamblia and Trichomonas vaginalis. These adaptations include reduction or elimination of most mitochondrial metabolic pathways and the use of oxidative stress enzymes generally associated with anaerobic prokaryotes. Phylogenomic analysis identifies evidence for lateral gene transfer of bacterial genes into the E. histolytica genome, the effects of which centre on expanding aspects of E. histolytica's metabolic repertoire. The presence of these genes and the potential for novel metabolic pathways in E. histolytica may allow for the development of new chemotherapeutic agents. The genome encodes a large number of novel receptor kinases and contains expansions of a variety of gene families, including those associated with virulence. Additional genome features include an abundance of tandemly repeated transfer-RNA-containing arrays, which may have a structural function in the genome. Analysis of the genome provides new insights into the workings and genome evolution of a major human pathogen. 相似文献
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The segment polarity network is a robust developmental module 总被引:42,自引:0,他引:42
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Synaptotagmins I and IV promote transmitter release independently of Ca(2+) binding in the C(2)A domain 总被引:5,自引:0,他引:5
At nerve terminals, a focal and transient increase in intracellular Ca(2+) triggers the fusion of neurotransmitter-filled vesicles with the plasma membrane. The most extensively studied candidate for the Ca(2+)-sensing trigger is synaptotagmin I, whose Ca(2+)-dependent interactions with acidic phospholipids and syntaxin have largely been ascribed to its C(2)A domain, although the C(2)B domain also binds Ca(2+) (refs 7, 8). Genetic tests of synaptotagmin I have been equivocal as to whether it is the Ca(2+)-sensing trigger of fusion. Synaptotagmin IV, a related isoform that does not bind Ca(2+) in the C(2)A domain, might be an inhibitor of release. We mutated an essential aspartate of the Ca(2+)-binding site of the synaptotagmin I C(2)A domain and expressed it in Drosophila lacking synaptotagmin I. Here we show that, despite the disruption of the binding site, the Ca(2+)-dependent properties of transmission were not altered. Similarly, we found that synaptotagmin IV could substitute for synaptotagmin I. We conclude that the C(2)A domain of synaptotagmin is not required for Ca(2+)-dependent synaptic transmission, and that synaptotagmin IV promotes rather than inhibits transmission. 相似文献