Calcium-induced cleavage of DNA topoisomerase I involves the cytoplasmic-nuclear shuttling of calpain 2

Important to the function of calpains is temporal and spatial regulation of their proteolytic activity. Here, we demonstrate that cytoplasm-resident calpain 2 cleaves human nuclear topoisomerase I (hTOP1) via Ca²⁺-activated proteolysis and nucleoplasmic shuttling of proteases. This proteolysis of hTOP1 was induced by either ionomycin-caused Ca²⁺ influx or addition of Ca²⁺ in cellular extracts. Ca²⁺ failed to induce hTOP1 proteolysis in calpain 2-knockdown cells. Moreover, calpain 2 cleaved hTOP1 in vitro. Furthermore, calpain 2 entered the nucleus upon Ca²⁺ influx, and calpastatin interfered with this process. Calpain 2 cleavage sites were mapped at K¹⁵⁸ and K¹⁸³ of hTOP1. Calpain 2-truncated hTOP1 exhibited greater relaxation activity but remained able to interact with nucleolin and to form cleavable complexes. Interestingly, calpain 2 appears to be involved in ionomycin-induced protection from camptothecin-induced cytotoxicity. Thus, our data suggest that nucleocytoplasmic shuttling may serve as a novel type of regulation for calpain 2-mediated nuclear proteolysis.     

依托地铁发展整合广州站域公共空间资源

广州地铁的网络化发展正在逐步走向成熟,城市公共空间在"以公交为导向"的城市规划理念(TOD)下,依托地铁发展,有效地整合功能和建立有地域性特征的发展模式是其中的关键环节.文中通过对现状和问题的分析、比较研究,用系统科学的方法,提出以地铁车站为核心、步行系统为联系纽带,形成网络化的站域公共空间体系.     

Meet-Cloud for Secure and Accurate Distribution of Negative Messages in Vehicular Ad hoc Network

Keeping Vehicular Ad hoc Network(VANET) from attacks requires secure and efficient distribution of information about bad entities. Negative messages are pieces of information that define the negative attributes of vehicles. By formally defining the negative message, we observe that accuracy is essential for its efficient distribution. We formally define the coverage percentage and accurate coverage percentage to describe the availability and distribution efficiency of negative message. These two metrics can jointly evaluate the performance of a distribution method. To obtain both high coverage percentage and high accurate coverage percentage, we propose meet-cloud, a scheme based on meet-table and cloud computing to securely and accurately distribute negative messages in VANET. A meet-table in a Road Side Unit(RSU) records the vehicles it encounters. All meettables are sent to cloud service to aggregate a global meet-table. The algorithm for distributing and redistributing negative messages are designed. Security analysis shows that meet-cloud is secure against fake and holding on to negative message attacks. Simulations and analysis demonstrate that meet-cloud is secure under denial of service and fake meet-table attacks. The simulation results also justify that meet-cloud outperforms the RSU broadcast and epidemic model.     

Quantitative Analysis of Multi-Recovery-Based Intrusion Tolerance Model

Quantitative analysis has always been a difficult problem in security analysis of intrusion tolerance systems. An intrusion tolerance model based on multiple recovery mechanisms is introduced in this paper and how to quantify the security attributes of the model is proposed. A state transition model with recovery states more accurately describes the dynamic behavior of the system. Considering that recovery mechanisms have a great impact on the security performance of the system, we set up the cost models corresponding to different recovery mechanisms. We propose a feasible security measure based on mean cost to security failure in order to evaluate the system cost during the recovery phase. The experimental results confirmed the feasibility of the proposed methods.     

一种适用于牧区的新型太阳能系统

我国是世界上地质灾害最严重的国家之一,对人民群众的生命财产安全构成了极大的威胁.湖南省又是我国地质灾害较严重的省份之一,且水系发育,河湖地地质灾害较多.本文以澧县为例,介绍了河湖地区管涌、岸崩的发育特征、分布规律及形成因素等,并提出了相应的防治措施,最后对河湖地质灾害进行了易发性分区,取得了较好的实际效果.结果表明:文中提出的方法对河湖地区的地质灾害防治具有一定的实际指导意义.     

略论蜀与滇的文化关系

我国西南地区部族众多,是典型的多民族地区。蜀与滇因为地域相邻,自古以来就关系密切。古蜀可能是西南地区最早创建的一个联盟之国,或称为宗主国。春秋战国时期,古蜀国的东方有巴国与楚国,北方有秦,这些都是当时势力比较强盛的列国。而在同时期的西南夷区域,夜郎与滇等依然是小邦,或者是邑聚之类的部族。古蜀国通过商贾与周边少数民族进行贸易以获取资源,此类商贸活动既有短程也有远程,产自于古蜀国的丝绸、青铜器、巴蜀的盐巴,以及其他很多物品,便通过商贸输入了滇国和西南夷地区,有些物品甚至经过远程贸易辗转贩卖到了南亚和中亚。与此同时,古蜀国成熟而高超的青铜铸造技术,也在商周之后随着商贸传入了滇国和西南夷地区。汉武帝大力经略西南夷,到东汉时期西南丝路已成为重要国际通道;后诸葛亮平定南中,有效地促进了汉族和西南各族的团结与融合,其意义是非常深远的。     

多胺基针刺布的制备及其在H2S去除中的应用

为了解决含硫化氢(H_2S)废气的高效净化问题,直接以腈纶针刺布为原材料,通过一步反应制备了多胺基针刺布,测得其交换容量为5.9 mmol/g,并用红外光谱和热分析技术对其进行了表征;研究了碱液存在下多胺基针刺布对H_2S的去除性能及气体流量、碱液浓度、增重率、氧含量和H_2S浓度等因素的影响,结果表明碱液和多胺基针刺布的协同作用对气体中的H_2S具有优异的去除性能.吸附饱和的多胺基针刺布可用碱液再生,且再生后性能无明显变化,在所设定实验条件下,针刺布处理容量和碱吸收容量分别稳定在30.6 mg/g和697.2 mg/g左右.     

基于未确知测度理论的定向铅钻遇采空区判识方法研究

老空水害事故是矿井安全生产的重要隐患之一。为确保矿井防治水安全,对老空区的探查与识别必不可少。文章立足于定向钻钻探新技术,根据定向钻观测内容及钻遇采空区的表现特征,确定了钻遇采空区的判识依据,基于未确知测度理论,结合实际案例,研究建立了钻遇采空区综合判识方法。研究结果表明,采用基于未确知测度理论的钻遇采空区判识方法在定向钻钻探过程中能够准确地识别采空区。研究成果对于指导定向钻判识采空区、完善定向钻探查老空区(包括老巷)探查技术体系具有重要参考价值。     

基于风廓线雷达的降水云分类方法在海南地区的应用

将风廓线雷达用于热带降水云体进行垂直探测,可以实时对热带地区降水云体进行较准确分类.通过对海口地区降水云系特征(2017年1月～12月)进行统计分析,提出一套基于风廓线雷达探测数据(回波强度、垂直速度和速度谱宽)的热带降水云体分类方法.以该方法为基础,结合天气雷达等观测资料,对2017年发生在海口地区的两次降水过程展开详细分析.结果表明:风廓线雷达具备较好体现降水云体垂直结构的能力,相比于天气雷达,风廓线雷达探测数据可以更精细地描述降水云系的变化趋势;相比于传统降水分类方案,采用以风廓线雷达探测数据为基础的分类方案可以提高对复杂降水类型识别的正确率;风廓线雷达可以更好地描述快速过境的高空微弱对流系统.     

Facile synthesis of flake-like dihydrate zinc oxalate particles

Monodispersed dihydrated zinc oxalate(ZnC_2O_4·2H_2O) particles with characteristic morphology were synthesized by aging a mixed solution of zinc nitrate(Zn(NO_3)_2) and sodium oxalate(Na_2C_2O_4) in the presence of a citrate ligand, with an average flat size of approximately 10–15 μm. The important parameters, including the solution pH values and the concentration of the zinc ions and citrate ligand, were investigated using a series of experiments. It is verified that the citrate ligand significantly affects the morphology of zinc oxalate particles, probably via its multiple roles of chelating, dispersing, and selective absorption. Thermodynamic equilibrium of the distribution of zinc species in an aqueous solution of Zn(Ⅱ)-citrate-oxalate-H_2O was estimated to explain the experimental results and to clarify the size and morphological evolution mechanism of the precipitated particles.