基于动态搜索区域的无线传感器网络小世界特性构建方案

为了减少无线传感器网络节点的能量消耗,以延长无线传感器网络的寿命,首先研究了如何通过在无线传感器网络中构建小世界特性以延长其网络寿命,并着重分析了DAS( directed angulation towards the sink)方案.然后针对DAS方案中临近sink节点区域内的节点能量过快消耗的问题,提出了一种在无线传感器网络中构建小世界特性的新方案——菱形区域方案.该方案根据sensor节点到sink节点距离的大小,动态地改变菱形捷径端点搜索区域的大小,达到构建小世界特性并增强网络抗毁性的目的.理论证明了菱形区域方案在一定条件下较DAS方案既节省能量又能延长网络的寿命.     

采用离散元方法评价集料的骨架结构

为了从细观结构角度深入研究骨架型沥青混合料集料的受力特征,在离散元程序PFC3D内,采用多球相互重叠的方法描述粗集料颗粒的三维不规则形状,并建立了考虑级配特征的集料混合物生成方法.按照集料粒径由大到小的顺序,在PFC3D中对各档集料进行了虚拟的逐级填充,并定量分析了各粒径集料对逐级填充后混合物中集料间隙率、集料之间的接触点数量、接触力等参数的影响.结果表明:集料混合物中,较粗集料受到的接触力大于较细集料;在较粗集料的混合物中,加入大于2.36 mm的较细集料有助于增加集料混合物的接触点数量,降低较粗集料所承担的接触力;粒径4.75 ～9.50 mm集料对接触点数量的增加最为显著;4.75 mm是骨架型沥青混合料的关键粒径.     

基于球磨机筒体振动的料位特征量

基于球磨机筒体的振动机理,提出了一种新的料位特征量.首先对球磨机筒体内料位分布的特点进行了分析,给出了球磨机筒体上振动测点的布置方案.同时针对筒体上振动信号采集的特点,开发了一套振动数据无线采集系统,确定了筒体振动信号的采集方法,并通过实验采集了不同给煤量工况下球磨机筒体与轴承座振动信号.然后根据料位与球磨机筒体及轴承座振动之间的关系对原始振动信号进行了滤波处理.最后从反映料位变化的敏感程度出发,比较了球磨机筒体与轴承座振动料位特征量.比较结果表明,相对于传统的料位特征量,筒体振动料位特征量更能灵敏地反映料位的变化,为实现料位的准确测量提供了可靠的依据.     

Marrow mesenchymal stem cells transduced with TPO/FL genes as support for ex vivo expansion of hematopoietic stem/progenitor cells

A new marrow-derived mesenchymal stem cell (hMSC) line that could support expansion of hematopoietic stem/progenitor cells (HSPCs) was developed. Primary hMSCs were infected with retrovirus containing Flt-3 ligand and thrombopoietin genes. CD34+ cells from cord blood were expanded with primary hMSCs or transduced hMSCs. The expansion of total nucleated cells, CD34+ cells and mixed colonies containing erythroid and myeloid cells and megakaryocytes for 2 weeks coculture with transduced hMSCs was remarkably increased. The outputs of long-term culture-initiating cells for 2 and 4 weeks coculture with transduced hMSCs were also largely increased. The expansion rates of HSPCs with transduced hMSCs were unchanged for 6 weeks. In contrast, the expansion rates of HSPCs with primary hMSCs declined drastically through 6 weeks. SCID-repopulating cell expansion with transduced hMSCs for 4 weeks was significantly higher than that of uncultured CD34+ cells and HSPCs expanded with primary hMSCs. Received 21 June 2005; received after revision 30 July 2005; accepted 24 August 2005     

Emodin inhibits tumor cell migration through suppression of the phosphatidylinositol 3-kinase-Cdc42/Rac1 pathway

Enhanced cell migration is one of the underlying mechanisms in cancer invasion and metastasis. Therefore, inhibition of cell migration is considered to be an effective strategy for prevention of cancer metastasis. We found that emodin (3-methyl-1,6,8-trihydroxyanthraquinone), an active component from the rhizome of Rheum palmatum, significantly inhibited epidermal growth factor (EGF)- induced migration in various human cancer cell lines. In the search for the underlying molecular mechanisms, we demonstrated that phosphatidylinositol 3-kinase (PI3K) serves as the molecular target for emodin. In addition, emodin markedly suppressed EGF-induced activation of Cdc42 and Rac1 and the corresponding cytoskeleton changes. Moreover, emodin, but not LY294002, was able to block cell migration in cells transfected with constitutively active (CA)-Cdc42 and CA-Rac1 by interference with the formation of Cdc42/Rac1 and the p21-activated kinase complex. Taken together, data from this study suggest that emodin inhibits human cancer cell migration by suppressing the PI3K-Cdc42/Rac1 signaling pathway.Received 7 February 2005; received after revision 11 March 2005; accepted 18 March 2005     

龙虾叶状幼体弧菌病防治的研究

对龙虾叶状幼体弧菌病采用多项措施，多种药物防治对比试验研究，总结出用沙－活性炭过滤海水，减少水体病原菌量，用漂白粉消毒水池和用具，杜绝污染源，用土毒素药浴亲虾，避免病原菌由卵带入，选用体格健壮的中年亲虾，保证孵化出的叶状幼体有旺盛的生命力，投放光合细菌、金藻、扁藻、丰年虫幼体、文蛤肉喂叶状幼体，改善叶状幼体营养，增强叶状幼体抗病力，利用光合细菌加速培育水体中有机物分解，净化水质，恶化病原菌生存环境     

Real time observation of the photocleavage of single DNA molecules

A method for real time observation of photo-cleavage of stratched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed.DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the Process of Photocleavage and relaxation of DNA under radiation of blue light is observed.We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules.The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity,cancer research as well as the interaction between DNA and dyes.     

Science Letters: A new quantitative model of ecological compensation based on ecosystem capital in Zhejiang Province, China

Ecological compensation is becoming one of key and multidiscipline issues in the field of resources and environmental management. Considering the change relation between gross domestic product (GDP) and ecological capital (EC) based on remote sensing estimation, we construct a new quantitative estimate model for ecological compensation, using county as study unit, and determine standard value so as to evaluate ecological compensation from 2001 to 2004 in Zhejiang Province, China. Spatial differences of the ecological compensation were significant among all the counties or districts. This model fills up the gap in the field of quantitative evaluation of regional ecological compensation and provides a feasible way to reconcile the conflicts among benefits in the economic, social, and ecological sectors.     

基于处方图的变量施肥作业系统设计与实现

设计一种由机载作业控制终端、DGPS、变量施肥控制器、电液比例阀及液压马达系统、地速信号采集单元和辅助平行作业导航单元等组成的变量施肥作业系统.设计基于PC104 CPU模块的机载作业控制终端并开发了基于处方图的变量作业控制和辅助导航软件.采用TI 2407型DSP开发变量施肥控制器,实现对阀控液压马达系统转速的闭环增量式PID控制算法.通过田间试验分析了系统的作业性能,结果表明:75 kg/hm2和225 kg/hm2两个常量施肥试验的施肥变异系数分别为16.83%和8.08%,与目标施肥量相比,平均施肥误差分别为2.98%和1.05%;0到375 kg/hm2的变量施肥试验表明,系统施肥位置滞后约为3.57 m,施肥变化延迟时间约为1.84 s.     

TiC掺杂对MCMB烧结体组织和性能的影响

以Tween 80为分散剂,分别以丙烯酰胺和N,N'-亚甲基双丙烯酰胺为单体和交联剂,采用凝胶注模工艺制备中间相碳微球(mesocarbon microbead,MCMB)片状素坯,再经过1 600 ℃的烧结,制备MCMB素坯烧结体试样.试验研究了粒度大小对粉体分散性、MCMB碳材料催化石墨化效果、烧结后MCMB碳材料层板物理性能的影响,并分析了产生这些影响的原因.研究结果表明:50 nm的TiC在MCMB烧结体中的分散效果好于15 μm的TiC;随着Ti质量分数的增加,掺杂50 nm 的TiC层板性能优于掺杂15 μm的层板;50 nm的TiC比15 μm的TiC更适合做MCMB的掺杂物.