A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

三种群捕食-食饵离散系统的持久性

研究了具有第Ⅱ类功能性反应的三种群捕食-食饵离散系统，得到了保证该系统持久性的充分条件．     

浅论医疗事业单位固定资产的规范化管理

剖析了医疗事业单位固定资产管理工作中存在的问题及其产生的原因,提出了医疗事业单位加强对固定资产进行规范化管理的若干建议。     

电厂汽轮发电机组漏氢处理

针对汽轮发电机组发电机漏氢严重使得安全生产受到极大威胁的情况,文章比较全面地分析了故障产生原因,给出了较好的解决办法,使发电机每天漏氢量大大减少,符合厂家及电力行业要求。     

库所耦合网的T-不变量求解算法

首先定义了库所耦合网及相关概念,其次揭示了库所耦合网N中各个分支网的T-不变量同N的T-不变量之间的关系,然后给出了一个库所耦合网的T-不变量求解算法,最后将该算法实现并给出初步实验数据,以说明所提算法比现有算法节省计算时间和存储空间。     

浅论钻探工艺与台班效益

通过对某公路勘察过程中两种不同钻探工艺的分析,浅论了钻探工艺与钻探台班效益的关系。     

东露天铁路专线采空区治理对策研究

针对东露天铁路专线所经过的采空区特点,分析了典型采空区上覆岩层的稳定性和上覆岩层移动形式,提出该地区采空区的治理措施。     

ARP欺骗攻击的整网解决方案研究

随着各种病毒与木马程序的泛滥,ARP欺骗攻击在校园网中已经成为危害网络正常稳定运行的主要问题.本文在对ARP协议进行深入分析的基础上,提出一种全网设备联动实现整体防御ARP欺骗的可行方案.     

UNIX系统源语言调试器的分析

源语言调试器是程序员不可缺少的软件开发工具。本文从介绍UNIX系统程序调试的基本思想入手,较详细地分析了UNIX系统源语言调试器的实现,阐述了源语言调试器实现中的几个主要问题。     

ADC的结构及其应用

介绍了目前流行的ADC的4种结构及其性能特点。并从其电路实现的复杂程度、采样速度和应用领域等方面进行了对比研究．