Elafin重组腺病毒载体在气道上皮细胞中表达的时相性变化研究

为研究elafin重组腺病毒表达载体Ad～elafin转染气道上皮细胞的特性，探讨其在上皮细胞中表达的时相性变化，运用①应用原代培养技术培养人气道上皮细胞；②Ad—elafin表达载体转染气道上皮细胞；③在转染后不同的时相中，采用荧光显微镜观察报告基因GFP的表达，ELISA法单克隆抗体检测细胞上清液elafin蛋白的表达，Northern膜杂交检测elafin基因mRNA的表达量等方法进行研究．发现：①原代培养人气道上皮细胞约5d即可作为靶细胞。②转染Ad—elafin24h时，荧光显微镜下可见上皮细胞内有少量的GFP表达，随着时间的延长，细胞内荧光逐渐增加，96h时GFP荧光阳性的细胞约为80％。③转染24～96h，实验组细胞上清液elafin的含量高于对照组（p〈0．01）；同时elafinmRNA的水平高于对照组（P〈0．05，0．01），且elafin表达及elafinmRNA水平在一定时间内呈时间依赖性关系。结论：Elafin重组腺病毒表达载体Ad—elafin成功转染人气道上皮细胞，目的基因不仅能在上皮细胞中表达，而且elafin蛋白具有细胞外分泌作用．     

热型连铸工艺凝固过程的数值模拟

采用直接差分的方法对热型连铸工艺凝固过程的稳定温度场进行了模拟,绘制了热型连铸工艺凝固过程的温度曲线,分析了铸型出口温度、冷却条件、连铸速度和型内金属液温度对凝固温度曲线的影响．铸型出口温度、冷却条件、连铸速度依次为影响液固界面的主要因素,型内金属液温度对液固界面的影响很小．     

不同种植年限果园土壤有机质变化特征研究

选取吉县0年、1年、6年、8年、11年和19年苹果园的土壤作为供试材料,分析土壤有机质含量的变化规律.研究结果表明:不同种植年限果园土壤有机质含量变化呈正态分布,且随种植年限增加,呈先下降后上升的趋势,距树干不同距离的土壤有机质含量变化均呈现这一趋势.     

Production of 316L stainless steel implant materials by powder metallurgy and investigation of their wear properties

In this study,the mechanical and wear properties of AISI 316L stainless steel implant materials,produced by powder metallurgy(P/M),were investigated.AISI 316L stainless steel powder was cold-pressed with 800 MPa of pressure and then sintered at 1200,1250 and 1300°C for 30 min as three sample groups.The microstructure,and mechanical and wear properties of the resulting steels were investigated.Light optical and scanning electron microscopiese were used to characterize the microstructure of the steels.Room temperature mechanical properties of the steels were determined by hardness measurements and impact tests.Wear was determined using the pin-on-disc wear test,and the results were evaluated according to weight loss.The results indicate that the sintering temperature,time and atmosphere are important parameters that affect the porous ratio of materials produced by P/M.Sintering at high temperature can eliminate small pores and make the residual pores spherical.The wear tests showed that the wear of the AISI 316L stainless steel implants changed depending on the sintering temperature and load.Spherical pores in the samples increase the wear resistance.Moreover,decreasing the porosity ratio of these materials improves all of their mechanical properties.     

基于无源辨识器的随机非线性系统自适应跟踪模块化设计

运用基于估计的模块化设计思想,研究了受方差不确定Wiener噪声干扰的参数严格反馈非线性系统对已知信号的自适应跟踪问题.构造控制Lyapunov函数,设计了具有鲁棒稳定特性的输入状态稳定控制器,确保系统满足控制器-辨识器分离.对不确定方差采用自适应方式处理.根据无源性理论,设计无源辨识器模块,给出了参数自适应律,确保系统误差的有界性,使跟踪误差在概率意义下收敛.     

循环冗余校验算法分析和实现

在网络中传输报文时,噪声干扰或传输中断等因素往往使接收端收到的报文出现错码。为了及时可靠地把报文传输给对方并有效地检测错误,需要采用差错控制。循环冗余校验CRC（Cyclic Redundancy Check）是由分组线性码分支而来,其主要应用是二元码组。循环冗余校验CRC编码简单且误判概率很低,在通信系统中得到了广泛的应用。文中详细介绍了循环冗余校验CRC的差错控制原理及其实现方法。     

Microsatellite instability in gastric cancer: molecular bases,clinical perspectives,and new treatment approaches

Gastric cancer is one of the most aggressive malignancies, with limited treatment options in both locally advanced and metastatic setting, resulting in poor prognosis. Based on genomic characterization, stomach tumour has recently been described as a heterogeneous disease composed by different subtypes, each of them with peculiar molecular aspects and specific clinical behaviour. With an incidence of 22% among all western gastric tumour cases, stomach cancer with microsatellite instability was identified as one of these subgroups. Retrospective studies and limited prospective trials reported differences between gastric cancers with microsatellite stability and those with instability, mainly concerning clinical and pathological features, but also in regard to immunological microenvironment, correlation with prognostic value, and responses to treatment. In particular, gastric cancer with microsatellite instability constitutes a small but relevant subgroup associated with older age, female sex, distal stomach location, and lower number of lymph-node metastases. Emerging data attribute to microsatellite instability status a favourable prognostic meaning, whereas the poor outcomes reported after perioperative chemotherapy administration suggest a detrimental role of cytotoxic drugs in this gastric cancer subgroup. The strong immunogenicity and the widespread expression of immune-checkpoint ligands make microsatellite instability subtype more vulnerable to immunotherapeutic approach, e.g., with anti-PD-L1 and anti-CTLA4 antibodies. Since gastric cancer with microsatellite instability shows specific features and clinical behaviour not overlapping with microsatellite stable disease, microsatellite instability test might be suitable for inclusion in a diagnostic setting for all tumour stages to guarantee the most targeted and effective treatment to every patient.     

氯化钙对洋桔梗切花瓶插期间的保鲜效果和生理效应

以不同浓度的氯化钙(CaCl2)溶液作为保鲜液,对洋桔梗切花进行瓶插试验,研究其对切花形态变化和体内生理变化的影响.结果表明:适宜浓度的CaCl2溶液能明显延长洋桔梗切花保鲜时间,增加花枝鲜质量,保持切花的水分平衡,延缓呼吸高峰的出现,降低呼吸速率,延缓蛋白质降解与细胞膜相对透性的增大.其中,以400 mg/L处理的保鲜效果最佳,可使切花瓶插寿命比对照延长3.5 d.     

基于数字喷泉码的水下传感器网络可靠传输与分析

水声通信具有低带宽、长时延、高误码率等特性,给水下传感器网络的可靠数据传输带来了巨大挑战。通过分析水下传感器网络的特点及传统可靠传输机制在水下传感器网络的应用局限性,提出一种基于数字喷泉码的、逐跳的可靠传输机制,并对译码成功的概率、端到端时延及能耗等网络性能进行了分析与仿真。结果表明,该机制明显减少了端到端时延和能耗,提高了包传输率。     

城市交通拥挤与系统分担交通理论的应用

应用系统分担交通理论,提出了解决城市交通拥挤的新途径。一是分散布局交通拥挤点以提高系统的整体效率;二是发展城市地下小汽车道路系统,形成地下以小汽车和地铁并存、地面以公共汽车及私人交通车为主、地上以轻轨为主的多层次、多功能的立体交通形式;三是大力推进交通需求管理,达到交通总量削减的目的。