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31.
Ferrari D Zalfa C Nodari LR Gelati M Carlessi L Delia D Vescovi AL De Filippis L 《Cellular and molecular life sciences : CMLS》2012,69(7):1193-1210
Cell therapy is reaching the stage of phase I clinical trials for post-traumatic, post-ischemic, or neurodegenerative disorders,
and the selection of the appropriate cell source is essential. In order to assess the capacity of different human neural stem
cell lines (hNSC) to contribute to neural tissue regeneration and to reduce the local inflammation after an acute injury,
we transplanted GMP-grade non-immortalized hNSCs and v-myc (v-IhNSC), c-myc T58A (T-IhNSC) immortalized cells into the corpus
callosum of adult rats after 5 days from focal demyelination induced by lysophosphatidylcholine. At 15 days from transplantation,
hNSC and T-IhNSC migrated to the lesioned area where they promoted endogenous remyelination and differentiated into mature
oligodendrocytes, while the all three cell lines were able to integrate in the SVZ. Moreover, where demyelination was accompanied
by an inflammatory reaction, a significant reduction of microglial cells’ activation was observed. This effect correlated
with a differential migratory pattern of transplanted hNSC and IhNSC, significantly enhanced in the former, thus suggesting
a specific NSC-mediated immunomodulatory effect on the local inflammation. We provide evidence that, in the subacute phase
of a demyelination injury, different human immortalized and non-immortalized NSC lines, all sharing homing to the stem niche,
display a differential pathotropism, both through cell-autonomous and non-cell autonomous effects. Overall, these findings
promote IhNSC as an inexhaustible cell source for large-scale preclinical studies and non-immortalized GMP grade hNSC lines
as an efficacious, safe, and reliable therapeutic tool for future clinical applications. 相似文献
32.
Massimo Ferrari 《Studies in history and philosophy of science》2012,43(1):18-26
This paper provides a critical evaluation of Friedman’s arguments in favour of a relativized a priori resting on Cassirer’s Neo-Kantianism, Reichenbach’s and Carnap’s constitutive a priori, and finally Kuhn’s account of scientific paradigms change. The main objection concerns Cassirer’s own view of dynamic and historical moveable a priori categories, which Friedman seems to underestimate and recasts in a merely regulative function. However, Cassirer conception of a “liberalized” a priori can shed new light on the process of scientific change and his transcendental method may be considered as a still stimulating alternative to Kuhn’s and post-Kuhnian relativism in the philosophy of science. 相似文献
33.
Failure to correct murine muscular dystrophy. 总被引:17,自引:0,他引:17
34.
35.
S. Curello C. Ceconi C. Bigoli R. Ferrari A. Albertini C. Guarnieri 《Cellular and molecular life sciences : CMLS》1985,41(1):42-43
Summary In the isolated and perfused rabbit heart ischemia induced a rapid decline of contractility, associated with a reduction of the content of tissue GSH with no significant changes in GSSG. Reperfusion induced a small recovery of contractility, a substantial release of total glutathione and a further decrease in the content of tissue GSH with a significant increase of tissue GSSG. Glutathione reductase and glutathione peroxidase activities were not affected by ischemia and reperfusion. This study suggests a possible role for glutathione in the determination of functional damage induced by myocardial ischemia and reperfusion.This study was supported by a grant from Ministero Pubblica Istruzione and CNR Rome (no 8202331.56). 相似文献
36.
In a rat model of hemorrhagic shock which caused the death of all control rats within 30 min, i.v. injection of the ganglion-stimulating drug dimethylphenylpiperazinium (DMPP) caused a dose-dependent reversal of the shock condition--without the need for reinfusion of the shed blood--starting from the dose of 4 ng/kg i.v. Shock reversal was associated with the mobilization of residual blood and improvement in blood flow, particularly at the carotid level. These results could influence our thinking on pathophysiology and first-aid management of shock. 相似文献
37.
L. De Caro G. Rindi V. Perri G. Ferrari 《Cellular and molecular life sciences : CMLS》1956,12(8):300-302
Riassunto La Piritiamina, somministrata per bocca in una singola dose di 0,5 mg a topolini di 12–14 g che ricevono 2 µg di tiaminapro die, non produce la sindrome neuromuscolare caratteristica dell'avitaminosi B1 e non modifica nè la piruvicemia nè il livello di vitamina nel fegato, mentre lo fa abbassare nel muscolo e nel cervello. Se i topolini non ricevono tiamina, la stessa dose di Piritiamina dà perdita di peso, sindrome neuromuscolare in tutti gli animali trattati e cospicuo abbassamento del livello di vitamina B1 nel cervello. Nelle stesse condizioni sperimentali, l'Ossitiamina, somministrataper os una sola volta in dosi di 0,5 e 2 mg, non dà alcuna sintomatologia neuromuscolare e non modifica nè il peso corporeo, nè il piruvato ematico, nè il contenuto in vitamina B1 del muscolo e del cervello, solo abbassa il livello vitaminico del fegato.Questi risultati non sono in favore dell'ipotesi di una funzione della tiamina distinta da quella della cocarbossilasi. 相似文献
38.
S. Guarini C. Bazzani S. Tagliavini A. Bertolini W. Ferrari 《Cellular and molecular life sciences : CMLS》1992,48(7):663-667
In a rat model of hemorrhagic shock which caused the death of all control rats within 30 min, i.v. injection of the ganglion-stimulating drug dimethylphenylpiperazinium (DMPP) caused a dose-dependent reversal of the shock condition-without the need for reinfusion of the shed blood-starting from the dose of 4 ng/kg i.v. Shock reversal was associated with the mobilization of residual blood and improvement in blood flow, particularly at the carotid level. These results could influence our thinking on pathophysiology and first-aid management of shock. 相似文献
39.
Orsomando G Lorenzi M Ferrari E de Chiara C Spisni A Ruggieri S 《Cellular and molecular life sciences : CMLS》2003,60(7):1470-1476
The phytotoxic protein PcF (Phytophthora
cactorum-Fragaria) is a 5.6-kDa cysteine-rich, hydroxyproline-
containing protein that is secreted in limited amounts by P. cactorum, an oomycete pathogen of
tomato, strawberry and other relevant crop plants. Although we have shown that pure PcF triggers
plant reactivity, its mechanism of action is not yet understood. Here we show that PcF, like other
known fungal protein elicitors involved in pathogen-plant interaction, stimulates the activity of
the defense enzyme phenylalanine ammonia a key step in understanding the mechanism of action of
PcF at a molecular level is knowledge of its three-dimensional structure, we overexpressed this
protein extracellularly in Pichia pastoris. The preliminary
structural and functional characterization of a recombinant PcF homologue, N4-rPcF, is reported.
Interestingly, although N4-rPcF is devoid of proline hydroxylation and has four additional
amino acid residues attached to its N terminus, its secondary structure and biological activity are
indistinguishable from wild-type PcF.Received 22 February 2003; received after revision 25 March 2003; accepted 14 April 2003 相似文献
40.
Ferrari Fernanda Menezes Fares Carolina Bordignon Martinelli Dante Pinheiro 《Systemic Practice and Action Research》2002,15(1):51-66
This paper aims to describe the application of Soft Systems Methodology (SSM) to the reality of a Brazilian enterprise. Systemic practices are ways of putting to use systemic thought in the real world, using the precepts of the systemic approach, which is very important for knowledge management and globalization. The systemic practice SSM is used in the resolution of ill-defined problems, which often involve behavioral variables and, thus, cannot be addressed by the so-called hard methodologies. In this paper, SSM is applied to the problematic of a middle-market business, an auto supply distributor, whose managers consulted the authors of this paper as regards proposing improvements of its performance. As a result of this work, the authors were able to propose core changes in attitudes, leading to changes in structure and process. 相似文献