全文获取类型
收费全文 | 29579篇 |
免费 | 69篇 |
国内免费 | 97篇 |
专业分类
系统科学 | 309篇 |
丛书文集 | 603篇 |
教育与普及 | 67篇 |
理论与方法论 | 119篇 |
现状及发展 | 13236篇 |
研究方法 | 1223篇 |
综合类 | 13770篇 |
自然研究 | 418篇 |
出版年
2013年 | 150篇 |
2012年 | 399篇 |
2011年 | 863篇 |
2010年 | 181篇 |
2008年 | 516篇 |
2007年 | 573篇 |
2006年 | 535篇 |
2005年 | 555篇 |
2004年 | 534篇 |
2003年 | 535篇 |
2002年 | 468篇 |
2001年 | 925篇 |
2000年 | 905篇 |
1999年 | 561篇 |
1992年 | 528篇 |
1991年 | 398篇 |
1990年 | 468篇 |
1989年 | 433篇 |
1988年 | 425篇 |
1987年 | 441篇 |
1986年 | 441篇 |
1985年 | 580篇 |
1984年 | 424篇 |
1983年 | 342篇 |
1982年 | 331篇 |
1981年 | 356篇 |
1980年 | 408篇 |
1979年 | 916篇 |
1978年 | 717篇 |
1977年 | 713篇 |
1976年 | 600篇 |
1975年 | 642篇 |
1974年 | 902篇 |
1973年 | 767篇 |
1972年 | 752篇 |
1971年 | 861篇 |
1970年 | 1133篇 |
1969年 | 962篇 |
1968年 | 895篇 |
1967年 | 889篇 |
1966年 | 780篇 |
1965年 | 572篇 |
1964年 | 167篇 |
1959年 | 345篇 |
1958年 | 568篇 |
1957年 | 371篇 |
1956年 | 341篇 |
1955年 | 297篇 |
1954年 | 335篇 |
1948年 | 216篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
251.
鸟害闪络是引起高压输电线路跳闸的主要原因之一.采用实地抽样调查的方法,对吉林省9个地区1 518基220kV高压杆塔上防鸟害设施的种类、损毁状况、防鸟效果和存在的问题进行了研究.结果表明:吉林省目前主要应用障碍性防鸟设施防止鸟类在输电杆塔上栖落和筑巢,以风力驱鸟器、鸟刺、滚动针式驱鸟器和防鸟罩为主;调查杆塔安装比例为53.6%,各地区存在差异;平均每基杆塔安装的防鸟设施数量为4.8个;不同类型防鸟设施损毁率不同,平均损毁率约为18.0%,其中带鸟刺风力驱鸟器和普通风力驱鸟器损坏率较高,分别为27.5%和27.3%.安装防鸟设施未能完全阻止鸟类在输电杆塔筑巢,但可在一定程度上改变鸟类在输电杆塔上的筑巢位置进而减少鸟害闪络的发生几率. 相似文献
252.
Direct detection of more than 50% of the Duchenne muscular dystrophy mutations by field inversion gels 总被引:5,自引:0,他引:5
Duchenne muscular dystrophy (DMD) is an X-linked disorder affecting about 1 in 3,500 males. It is allelic with the milder Becker muscular dystrophy. The biochemical basis for both diseases is unknown and no effective treatment is available. Long-range physical mapping has shown that the DMD gene, localized in Xp21, is extremely large, exceeding 2 million base pairs. Until now, carrier detection and prenatal diagnosis has involved the use of linked restriction fragment length polymorphism markers which detect muscular dystrophy-associated deletions in about 10% of the cases. Field inversion gel electrophoresis (FIGE) allows the detection of structural rearrangements in 21 out of 39 of the DMD patients studied (54%), of which 14 (65%) were not detected by conventional methods. Large deletions seem to make up a much higher fraction of the DMD mutations than so far indicated by other methods. A region prone to deletion was located in the distal half of the gene. FIGE analysis could provide a valuable extension of information for carrier detection and prenatal diagnosis. The technique should be generally applicable to the study of diseases involving structural chromosomal rearrangements. 相似文献
253.
HIV preferentially infects HIV-specific CD4+ T cells 总被引:34,自引:0,他引:34
Douek DC Brenchley JM Betts MR Ambrozak DR Hill BJ Okamoto Y Casazza JP Kuruppu J Kunstman K Wolinsky S Grossman Z Dybul M Oxenius A Price DA Connors M Koup RA 《Nature》2002,417(6884):95-98
HIV infection is associated with the progressive loss of CD4(+) T cells through their destruction or decreased production. A central, yet unresolved issue of HIV disease is the mechanism for this loss, and in particular whether HIV-specific CD4(+) T cells are preferentially affected. Here we show that HIV-specific memory CD4(+) T cells in infected individuals contain more HIV viral DNA than other memory CD4(+) T cells, at all stages of HIV disease. Additionally, following viral rebound during interruption of antiretroviral therapy, the frequency of HIV viral DNA in the HIV-specific pool of memory CD4(+) T cells increases to a greater extent than in memory CD4(+) T cells of other specificities. These findings show that HIV-specific CD4(+) T cells are preferentially infected by HIV in vivo. This provides a potential mechanism to explain the loss of HIV-specific CD4(+) T-cell responses, and consequently the loss of immunological control of HIV replication. Furthermore, the phenomenon of HIV specifically infecting the very cells that respond to it adds a cautionary note to the practice of structured therapy interruption. 相似文献
254.
两种前处理方法测定菜籽油中6种金属元素含量的比较 总被引:1,自引:0,他引:1
目的:比较不同前处理方法对菜籽油中6种金属元素含量测定的影响.方法:分别使用干灰化法和湿消化法进行前处理,并用火焰原子吸收分光光度法分别测定15个菜籽油样品中铅、镉、铜、铁等6种金属元素的含量.结果:干灰化法和湿消化法处理后所测菜籽油样品中Pb、Cu含量差异无统计学意义(P>0.05),Fe、Mn含量差异有统计学意义(P<0.05).各元素的R2均大于0.99,干灰化法的加标回收率81.90%~107.45%,湿消化法的加标回收率99.90%~108.05%.均满足痕量分析要求,相比之下,干灰化法与湿消化法的加标回收率比较差异有统计学意义(P<0.05),且湿消化法-火焰原子吸收分光光度法测定油样的回收率相对干灰化法的要高.结论:两种方法都能获得准确的测定结果;湿消化法要优于干灰化法. 相似文献
255.
A universal marker for transgenic insects 总被引:16,自引:0,他引:16
256.
Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells 总被引:102,自引:0,他引:102
R L Williams D J Hilton S Pease T A Willson C L Stewart D P Gearing E F Wagner D Metcalf N A Nicola N M Gough 《Nature》1988,336(6200):684-687
Embryonic stem (ES) cells, the totipotent outgrowths of blastocysts, can be cultured and manipulated in vitro and then returned to the embryonic environment where they develop normally and can contribute to all cell lineages. Maintenance of the stem-cell phenotype in vitro requires the presence of a feeder layer of fibroblasts or of a soluble factor, differentiation inhibitory activity (DIA) produced by a number of sources; in the absence of DIA the ES cells differentiate into a wide variety of cell types. We recently noted several similarities between partially purified DIA and a haemopoietic regulator, myeloid leukaemia inhibitory factor (LIF), a molecule which induces differentiation in M1 myeloid leukaemic cells and which we have recently purified, cloned and characterized. We demonstrate here that purified, recombinant LIF can substitute for DIA in the maintenance of totipotent ES cell lines that retain the potential to form chimaeric mice. 相似文献
257.
258.
Male development of chromosomally female mice transgenic for Sry 总被引:117,自引:0,他引:117
The initiation of male development in mammals requires one or more genes on the Y chromosome. A recently isolated gene, termed SRY in humans and Sry in mouse, has many of the genetic and biological properties expected of a Y-located testis-determining gene. It is now shown that Sry on a 14-kilobase genomic DNA fragment is sufficient to induce testis differentiation and subsequent male development when introduced into chromosomally female mouse embryos. 相似文献
259.
260.
吴畏 《华中科技大学学报(自然科学版)》1994,(10)
阐述了轴向磁场盘式永磁无刷直流发电机的结构及其特点,提出了气隙磁场的简化模型,并对三维磁场进行了分析,在此基础上探讨了这种电机的设计方法,以最大功率输出为优化目标,导出了几个简单实用的设计公式。6极9相样机的实测数据证实了分析和设计方法的正确性,这种无刷直流发电机结构紧凑,效率高,特别适用于由引擎驱动的发电系统。 相似文献