New Challenge of Global Change

We briefly review the development of global change research program in the last decade, introduce the new structure of global change in the next decade, discusses the opportunities and challenges for China, and gives some suggestion on the supporting fields during the Tenth Five-Year Plan Period from 2001 to 2005.     

香港竹类植物检索

根据美国哈佛大学胡秀英博士和她在香港植物标本室的同事们近年采集、鉴定的标本和有关资料,对香港产的竹类植物13属、46种、6变种、1变型和4个栽培变种,作了检索.     

结构动态分析中的可视化技术

提出了结构动态分析的可视化思路,并在先进的图形开发环境下利用OpenGL作为三维图形的绘制引擎,实现了结构的动态模拟。     

Efficient Composition of Semantic Web Services with End-to-End QoS Optimization

The efficiency of QoS-aware service composition is important since most service composition problems are known to be NP-hard. With the growing number of web services, service composition is like a decision problem on selecting services or/and execution plans to satisfy the users' end-to-end QoS requirements (e.g. response time, throughput). Composite services with the same functionality may have different execution plans, which may cause different end-to-end QoS. This paper presents a model combining semantic data-links and QoS, which leads to an efficient approach to automatic construction of a composite service with optimal end-to-end QoS. The approach is based on a greedy algorithm to select both services and execution plans for composite services. Empirical and theoretical analyses of the approach show that its time complexity is O(mn²) for a repository with n services and an ontology with m concepts. Moreover, the approach increases linearly in time when using an index to search services in the repository. Tests with a repository with 20 000 services and an ontology with 300 000 concepts show that the algorithm significantly outperforms current existing algorithms in terms of composition efficiency while achieving optimal end-to-end QoS.     

hIL-2／IFN-1嵌合基因原核表达质粒的构建与表达

为构建hIL 2 /IFN γ 嵌合基因原核表达质粒 ,筛选其高效表达工程菌 ,设计特异性引物 ,PCR扩增目的基因并克隆至pPROEXHTb质粒NcoⅠ /HindⅢ位点 ,经双酶切及序列测定筛选阳性克隆 ,IPTG诱导工程菌后以SDS PAGE电泳鉴定目的蛋白的表达情况 ,并测定其抗原性和生物学活性 ,结果成功构建了工程菌Top10F’[hIL 2 /IFN γ],经优化表达条件后 ,目的蛋白表达量约占菌体总蛋白的 30 % ,分子质量约为 34ku .ELISA和Western blot实验结果表明 ,目的蛋白能特异性结合抗IFN γ 抗体 ,呈阳性反应 ,生物学活性测定显示其IL 2和IFN γ 比活性分别为 1 7× 10 7U/mg与 3 2× 10 6 U/mg .     

低合金钢过冷奥氏体转变曲线CAD

用计算机对低合金钢的过冷奥氏体转变动力学进行了数学模拟，综合考 虑了冷却速度、合金化学成分和晶粒度等因素对过冷奥氏体转变的影响。利 用ｄＢＡＳＥ Ⅲ程序设计语言和ＢＡＳＩＣＡ程序设计语言建立了合金钢数据库， 并在ＩＢＭ－ＰＣ机上绘出了ＴＴＴ曲线及ＣＣＴ曲线。     

The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans

In metazoans, most pre-messenger RNAs contain introns that are removed by splicing. The spliced mRNAs are then exported to the cytoplasm. Recent studies showed that splicing promotes efficient mRNA export, but the mechanism for coupling these two processes is not known. Here we show that Aly, the metazoan homologue of the yeast mRNA export factor Yralp (ref. 2), is recruited to messenger ribonucleoprotein (mRNP) complexes generated by splicing. In contrast, Aly does not associate with mRNPs assembled on identical mRNAs that already have no introns or with heterogenous nuclear RNP (hnRNP) complexes. Aly is recruited during spliceosome assembly, and then becomes tightly associated with the spliced mRNP. Aly shuttles between the nucleus and cytoplasm, and excess recombinant Aly increases both the rate and efficiency of mRNA export in vivo. Consistent with its splicing-dependent recruitment, Aly co-localizes with splicing factors in the nucleus. We conclude that splicing is required for efficient mRNA export as a result of coupling between the splicing and the mRNA export machineries.     

Seasonal variation of snow microbial community structure in the East Rongbuk glacier, Mt. Everest

Mt. Everest, the zenith of the world, plays an impor- tant role in the global climate change. During the lastdecade, some studies on glacier and snow have been undertaken in this region. The climate and environment changes in the past hundred years have b…     

同义密码子的频率分布

本文提出了关于同义密码子非无规分布的一个机制,从主方程出发,如果只考虑同义突变由图论方法证明了密码子的均匀分布,但是如果考虑了不同氮基酸间的突变,便可导出同义密码子的非无规分布。后者反映了选择约束对密码子分布的影响。     

一个分子序列的进化模型

本文提出了关于分子序列进化的一个简单模型,序列由于随机的点突变和一定的选择规则作用而不断变化,利用这个模型,可以解释进化中信息参数的下降,和的上升,并可解释序列具有短程的关联长度,对不同物种的出现,也可作初步讨论。