N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence

The L protein (241kD) of vesicular stomatitis virus (VSV) is the most important snbnnit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96—120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108—120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines.     

New geological evidence of crustal thickening in the Gangdese block prior to the Indo-Asian collision

Recent mapping in the Gangdese block has revealed many leucogranites that are similar to those in the High Himalaya. These leucogranites formed at ～140 Ma as indicated by monazite Th-Pb ion-microprobe dating and cooled at ～130 Ma as indicated by muscovite ^40Ar/^39Ar dating. In conjunction with previous structural and paleogeographic studies, the new data indicate that the Gangdese block underwent crustal thickening and associated exhumation during ～140—130 Ma. In this regard, the southern margin of Eurasia continent was comparable to the modern South American Altiplano-Puna plateau, the prime example of active ocean-continent subduction and associated thickened crust. Specifically, the early stages of crustal thickening and uplifting of the Gangdese block may result from subduction of the Neo-Tethyan Ocean. If the Tibetan Plateau would form by accretion of a series of blocks with thickened crust, an elevated topographic plateau similar to the Altiplano-Puna plateau had formed before collision between the Indian and Eurasian plates. Then the Tibetan Plateau would have quickly thickened, uplifted, and begun to extend soon after onset of the collision. Thus, the deformational mechanism of the Tibetan Plateau is not distributed shortening, but rather concentrating deformation within regions of thin crust between the accreted blocks.     

Contributions of different land cover types in Otindag Sandy Land and Bashang area of Hebei Province to the material source of sand stormy weather in Beijing

Different land cover types in Otindag Sandy Land and Bashang area of Hebei Province are linked to a material source of sand stormy weather in Beijing based on results of field vegetation and soil survey and laboratory works. Results of grain size analysis show that dust release potential in per unit area of moving sandy land is small,while lowland meadow and meadow steppe in stony hills have high potential of releasing dusts in per unit area during sand stormy weather occurrence. Further considering the effects of vegetation, it is inferred that the moving sandy land served as a material source of past dust storm and the possibility of dust release in per unit area is low in current time.Typical steppe in stony hills is undergoing desertification and its dust release possibility in per unit area is high. Farmland has strong potential of release dusts when they are ploughed in spring, but a large amount of therophytes grow and thus prevent dusts from release when cultivation was terminated.Potentials of dust release in per unit area in fixed sand dunes,stony mountain meadow steppe and low land meadow are lowdue to high cover of perennials. Sand dune reactivation and desiccation of lakes and lowlands under estimated future climatic change will make them serve as a future material source of sand stormy weather.     

鄂尔多斯盆地低渗透油田注水开发的调整与优化

根据鄂尔多斯盆地成功开发的主要特低渗油田——安塞、靖安、华池油田长6、长3油层的储层特征及开发实践,提出了改善特低渗油田注水开发效果的注采参数、注水方式优化及调整技术,从而达到提高单井产量及最终采收率,降低开发投资和生产成本,以提高整体开发效益的目的.     

电铲铲斗齿的修复工艺

通过分析铲斗齿的磨损原因，提出了采用堆焊的方法对铲斗齿进行修复。详细介绍了铲斗齿的修复工艺，堆焊材料的选择及堆焊时应注意的事项。实践表明，此修复工艺切实可行，取得了令人满意的效果。     

MPEG-2传输流再复用器的设计

提出一套MPEG—2传输流再复用器的设计方案。该方案采用CAM RAM组合代替简单的RAM结构用于构造PID信息表，在保证速度的基础上，大大节约了资源；提出了“两步法”PCR校正算法，在保证节目时钟基准(PCR)校正精度的基础上，大大减小了硬件设计的复杂度；基于该再复用器，提出了MPEG—2传输流、纯数据、主控机插入等几种实用的数据插入方式，以适应不同的应用环境。     

开关线性复合变换低阻输出特性对系统的优化

为了在现有电力电子技术基础上进一步提高功率变换器的综合性能，揭示了一种由电力电子开关电路与线性电路有机结合而派生的开关线性复合功率变换器(SLH)。简述了该类变换器的基本工作原理，列举了并联型和串联型两种电路拓扑及其特点，说明了SLH的低阻输出特性对提高功率变换器静动态性能的作用，并对SLH与典型的PWM LC滤波单元的变换器进行了比较分析，给出了用于电源和传动系统不同负载条件下的测试数据和典型波形。结果表明，SLH在高效与优波兼顾、多类负载适应性和抗负载扰动能力方面具有综合优化性能。     

有害生物综合治理(IPM)的潜在组成植物源农药

植物源农药对环境和非靶标生物安全,作用方式和机理特异及易获取等特点迎合了当代有害生物综合治理(IPM)发展,它能克服化学合成农药引起的副作用,有望成为有害生物综合治理(IPM)体系统的重要组成.     

低含水率沙床的临界起沙风速

针对目前对风沙起动机制的认识有限的现状，采用量钢分析法推导出对湿沙床的起沙有影响的4个无量纲数。经分析得到起沙风速与沙床沙粒粒径和沙床粘结力间的数量关系。通过建立沙床粘结力与含水率、沙粒粒径的关系式，得到临界起沙风速和沙粒粒径，含水率间的定量关系式。认为低含水率沙床的风沙起动风速与颗粒粒径的一次方和沙床含水率的1／4次方成线性关系，此结论与前人的实验结果符合得很好。     

梯度型聚合物光纤预制棒折射率分布的间接测试

利用紫外光对溴苯（BB）的吸收作用，采用紫外吸收光谱法对PMMA－BB梯度型聚合物光纤预制棒的折射率分布进行了间接测试，详细介绍了这种紫外有机光谱法的测试原理及方法，测试结果表明，所制备的预制棒的折射率分布是呈抛物线分布，但在最佳理论分布有着一定的偏差，与Lorentz-Lorenz模拟方程的结果相对接近。同其他复杂的测试方法相比，紫外光谱法所需仪器普通，测试简便，不失为实验室中较为简单易行的一种预制棒折射率分布测试方法。