一个基于沟通函数的谣言传播仿真模型

人际谣言传播过程中人与人之间的沟通以及个体的记忆是谣言传播的主要因素。引入了个体对瑶言态度的不确定性、谣言的重要性和模糊性等参数建立了个体间的沟通函数,以此力基础实现了一介基于有限记忆的人际谣言传播的Agent仿真模型。仿真结果表明：谣言内容的重要性和模糊性、人际间的信任程度以及个体传播谣言的倾向概率以及个体基于有限记忆的态度选择是形成谣言及传播的重要因素。此结果能够与社会学、传播学对谣言形成与传播的研究结果相印证,反映了模型的合理性和有效性。     

Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin

When used in combination with certain chemotherapies, curcumin has been shown to increase apoptosis in several cancer cell lines. Here, we report the combined effects of curcumin and cinobufacini on human cervical carcinoma cells. The aim of this study was to examine whether curcumin could enhance apoptosis induced by cinobufacini. 3-(4,5-Dimethylthiazol-2-y1)-2,5- diphenytetrazolium bromide (MTT) assays revealed that the growth and proliferation of HeLa cells could be inhibited by 75% after a combined treatment of 25 μg/mL cinobufacini and 8 μg/mL curcumin. The combined treatment is 3 times more effective than treatment with 25 μg/mL cinobufacini alone. Annexin V-FITC/PI staining, morphological changes and immunofluorescence verified a significant enhancement in cinobufacini-induced apoptosis when cells were also exposed to curcumin. The data showed that the proportion of early apoptotic cells significantly increased from 15.43% in cells treated only with 25 μg/mL cinobufacini to 49.2% in cells treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. Moreover, compared with treatment of only 25 μg/mL cinobufacini, ROS production increased 1.7-fold, the intracellular free Ca 2+ concentration increased 1.5-fold, and the mitochon- drial membrane potential decreased by 20% in the combined treatment. Simultaneously, the atomic force microscopy (AFM) re- sults suggest that cells treated with a combination of cinobufacini and curcumin varied significantly in shape and ultrastructure. Collapsed cells with leaking cytoplasm, blebbing pores and emerging apoptotic bodies were prevalent. The nanoparticle size increased from 70 nm when the cells were treated with 25 μg/mL cinobufacini to 190 nm when the cells were treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. The size increase resulted in the cell membrane becoming considerably rough. These results can improve our understanding of combination treatments. Specifically, the combination of cinobufacini and curcumin may potentially find use as a novel cervical carcinoma treatment. Additionally, AFM is a powerful tool that can be used to explore cellular morphologies and ultrastructures.     

基坑支护工程中的安全监理要点

据对基坑支护工程内容和基坑支护主要方式的的介绍,结合施工监理工作的实践,提出基坑支护工程中需要监理注意的监控要点。     

基于半解析法的泵房结构平面和空间内力分析比较

为了避免地基范围这一影响因素,采用半无限地基上的半解析法对淮阴三站泵房结构分别进行二维、三维的内力计算,突出分析二者的差异．对底板、流道墩墙及检修间墙体等关键结构做详细对比,从弯矩的分布规律和数值两方面进行了整体、局部异同的对比分析．由数值分析结果可知,平面分析并不能考虑上下游挡墙对侧墙、底板顺河向的影响,流道处的墙体在顺水向是变厚度的,很难找到合理的代表面,对大型泵房结构进行三维有限元计算分析是必要的．     

Alteration of the specificity ofPst I restriction endonuclease

The influence of factors on the substrate-specificity ofPst I restriction endonuclease has been studied with the method of electrophoresis. The results show that, the specificity ofPst I almost can not be influenced by the single alteration of the concentration of Tris·HCl, Mg²⁺ or Na⁺ in the reaction system, but it can be altered by the reduction of any two of them. The specificity can not be altered by the single alteration of pH or the replacement of Mg²⁺ with Mn²⁺. The addition of glycerol or dimethylsulphoxide (DM-SO) to the reaction system results in the relaxation of the substrate-specificity ofPst I, but dimethyl-methylformide, glycol and ethyl alcohol can not bring about the alteration ofPst I specificity. Through the method of cloning and sequencing, the nucleotides of No. 1 and 6 in the recognition sequence ofPst I have changed (1C→A or 6G→T). Used with the enzyme analysis of an artificially synthetic DNA segment containing a special sequence, the nucleotides of No. 1 and 6 have both changed (1C→A and 6G→T). The recognition sequence ofPst I is speculated to be changed from CTGCA→G to TGCA→. Foundation item: Supported by the Research Fund for the Doctoral Program of Higher Education. Biography: Zou Guo-lin (1947-), male, professor. research direction, biochemistry.     

Excel集成PHEES绘制水盐体系相图及模拟K2SO4生产工艺

利用Excel 97的VBA编程调用PHEES的单点固液相平衡计算模块,通过网格法绘制出Na+-K+-Cl--SO42--H2O的25℃和100℃下的相图和等水线图.并设计无机盐工艺模拟程序,模拟了K2SO4生产的一种两段法工艺.     

基于有限元数值模拟的直流电阻率激电找矿探测技术

为提高直流电阻率激电探测成果地质解释准确性,促进直流电阻率激电在找矿勘查中的应用,采用有限元数值模拟对直流电阻率激电探测中低阻高极化体、直立板状体、低阻盖层、高阻盖层、地表不均匀体、地形起伏及实测数据反演解释几个主要方面进行了对比分析。研究结果表明,对于实际勘查中的反演解释结果,根据异常特征构建地质体模型并进行有限元数值模拟,能快速判别探测目标体异常属性、区分虚假异常,提高定量解释水平及找矿勘查效率。研究结果为直流电阻率激电找矿探测定量解释提供了参考。     

带钢连续退火温度场数值模拟及其再结晶规律研究

利用有限元软件建立几何模型和有限元模型,对42CrMo带钢高温连续退火温度场进行数值模拟,通过稳态条件下带钢温度及再结晶软化率随时间变化规律的计算,研究非稳态条件下再结晶软化率随时间变化的关系。     

CSP生产线高速钢轧辊温度场有限元分析

采用有限元软件ANSYS,结合某CSP生产线F4机架工作辊表面温度的实测值,建立轧辊二维温度场模型,对轧辊在轧制过程中的温度和热凸度变化进行研究。结果表明,轧辊在咬入弧区的换热系数为5.8×104 W/(m2·K),在非咬入弧区的水冷等效换热系数为1.1×104 W/(m2·K);在此等效换热边界条件下,使轧辊热凸度达到稳定的烫辊时间约为75min。     

发酵床养殖—农村污染减排技术研究与应用

发酵床养殖是一种无污染、零排放的有机养殖技术.在专门的发酵床猪舍内,填入有机垫料,利用有益菌发酵降解猪的排泄物,可达到零排放的目的.推广应用该技术不仅可以增强猪的抗病能力,还可以提高品质,增加经济效益,同时显著提高农村污染减排效果.