高压浓相煤粉气力输送差压信号多分辨分析

为了探究高压浓相气力输送过程中时频空间的内部特征,利用六尺度多分辨小波分析对试验差压信号进行时频分解.引进能量、Shannon熵和标准差STD作为特征量对气固两相流频域空间进行分析.分析表明:浓相气力输送过程中,差压信号中的能量主要分布在低频区,Shannon熵从高频到低频上呈先减小后增大分布趋势.在相同的试验压力条件下,随着表观速度的增大,低频所占据的能量份额减小,信号脉动向高频移动;高频d1和d2的Shannon熵和a6上的STD增大,低频d4~d6上的Shannon熵和d1~d6上的STD减小.为进一步研究流型辨识及流动稳定性提供了新方法.     

醋酸纤维薄膜电泳法分离测定鸡血清蛋白质

应用醋酸纤维薄膜电泳法分离测定了鸡血清中不同蛋白质。对电流强度、电泳时间和点样量等影响分离效果的条件进行了选择。并用光度计和薄层扫描仪对样品进行了定量测定。     

基于漫水填充的图像骨架提取方法

针对当前图像骨架提取存在毛刺现象和骨架冗余的问题,提出基于漫水填充的图像骨架提取方法。对目标图像预处理,得到阈值化二值图像,分割出前景和背景;构建掩模图像和轴线图,为后续运算过程提供数据来源和判断依据;通过遍历水平面灰度直方图,模拟漫水过程,收缩掩膜图像的前景轮廓,根据邻域连通分量变化,确定是否为骨架特征点,待轮廓收缩完毕,得到图像骨架。实验验证表明,该方法可以有效地提取图像骨架,毛刺现象和冗余结构明显减少,执行速度快。     

通过空间相似度设置阈值的Douglas-Peucker算法

线状地物的化简是地图综合的重要组成部分,Douglas-Peucker算法作为一种经典的化简线状地物的算法,化简时需要进行多次实验才能选取到合适的阈值,得到最终化简结果。为了避免选取阈值繁琐的过程,通过计算不同阈值下化简前后线状地物间的空间相似度,并将绝对阈值转换为相对阈值,利用曲线拟合的方法得到相对阈值和空间相似度之间的关系。基于两者之间的关系,在使用Douglas-Peucker算法化简线状地物时,即可通过空间相似度来选取合适的化简阈值,提高了化简的效率。     

鄂东大别山生物多样性研究

对鄂东大别山区的生物多样性进行了研究.目前已知该区有维管植物195科663属1 461种,占湖北省总种数的24.27%和全国总种数的5.25%,属国家特有、珍稀濒危或重点保护的植物有34种,有苔藓植物30科64属143种.已知该区有陆生脊椎动物26目65科208种,占湖北省总种数的31.04%和全国总种数的8.09%,其中有27种国家重点保护野生动物和61种湖北省级重点保护动物.该区自然植被类型有10个植被型,52个群系.该区不仅动植物区系成分多样性复杂,而且经济类型多样.     

基于修正差别矩阵的高效属性约简算法

为降低基于修正差别矩阵的属性约简算法的复杂度,给出了基于修正差别矩阵的简化差别矩阵,证明了基于该简化差别矩阵的属性约简定义与基于原修正差别矩阵的属性约简定义是等价的.在此基础上设计了一个基于简化差别矩阵的属性约简算法,其空间和时间复杂度分别被降为O(|C|(|U'pos||U/C|))和max{O(|C|2(|U'pos||U/C|)),O(|C||U|log|U|)}.实例说明:用新算法进行属性约简,不仅减少了计算量,而且减少了存储空间,因而是一种高效的属性约简算法.     

Defining G protein-coupled receptor peptide ligand expressomes and signalomes in human and mouse islets

Introduction

Islets synthesise and secrete numerous peptides, some of which are known to be important regulators of islet function and glucose homeostasis. In this study, we quantified mRNAs encoding all peptide ligands of islet G protein-coupled receptors (GPCRs) in isolated human and mouse islets and carried out in vitro islet hormone secretion studies to provide functional confirmation for the species-specific role of peptide YY (PYY) in mouse islets.

Materials and methods

GPCR peptide ligand mRNAs in human and mouse islets were quantified by quantitative real-time PCR relative to the reference genes ACTB, GAPDH, PPIA, TBP and TFRC. The pathways connecting GPCR peptide ligands with their receptors were identified by manual searches in the PubMed, IUPHAR and Ingenuity databases. Distribution of PYY protein in mouse and human islets was determined by immunohistochemistry. Insulin, glucagon and somatostatin secretion from islets was measured by radioimmunoassay.

Results

We have quantified GPCR peptide ligand mRNA expression in human and mouse islets and created specific signalomes mapping the pathways by which islet peptide ligands regulate human and mouse GPCR signalling. We also identified species-specific islet expression of several GPCR ligands. In particular, PYY mRNA levels were ~ 40,000-fold higher in mouse than human islets, suggesting a more important role of locally secreted Pyy in mouse islets. This was confirmed by IHC and functional experiments measuring insulin, glucagon and somatostatin secretion.

Discussion

The detailed human and mouse islet GPCR peptide ligand atlases will allow accurate translation of mouse islet functional studies for the identification of GPCR/peptide signalling pathways relevant for human physiology, which may lead to novel treatment modalities of diabetes and metabolic disease.

     

Control of a key transition from prostrate to erect growth in rice domestication

The transition from the prostrate growth of ancestral wild rice (O. rufipogon Griff.) to the erect growth of Oryza sativa cultivars was one of the most critical events in rice domestication. This evolutionary step importantly improved plant architecture and increased grain yield. Here we find that prostrate growth of wild rice from Yuanjiang County in China is controlled by a semi-dominant gene, PROG1 (PROSTRATE GROWTH 1), on chromosome 7 that encodes a single Cys(2)-His(2) zinc-finger protein. prog1 variants identified in O. sativa disrupt the prog1 function and inactivate prog1 expression, leading to erect growth, greater grain number and higher grain yield in cultivated rice. Sequence comparison shows that 182 varieties of cultivated rice, including 87 indica and 95 japonica cultivars from 17 countries, carry identical mutations in the prog1 coding region that may have become fixed during rice domestication.     

2.5 Gbit/s PHEMT前置放大器噪声分析与验证

根据OMMIC公司通过测量得到的0.2μm GaAs PHEMT器件参数模型和噪声模型,设计了2.5 Gbit/s的共源跨阻前置放大器.并根据PHEMT晶体管Y参数下的噪声模型,结合Y参数和ABCD参数下的噪声密度矩阵,分析了电路在带有晶体管噪声源情况下整个电路的噪声电压,得出了共源跨阻前置放大器等效输入噪声电流密度的理论公式.实现了芯片制作,并且对芯片进行了噪声参数的测量,测量结果、仿真结果和理论分析结论在6GHz的频率范围内基本符合.     

煤粉高压密相气力输送特性

在输送压力可达4.0MPa,固体输送流率达840kg/h的仓式泵高压气力输送试验装置上进行粉煤高压浓相气力输送试验研究.考察了流化风量、充压风量、输送总差压对煤粉输送量、固气比的影响.研究表明:随着流化风量的增大,煤粉输送量逐渐增大,固气体积比先增大然后减小;当煤粉在发料罐内被充分流化后,再加大充压风量,固气体积比逐渐减小,而煤粉输送量略有增加;输送总压差对煤粉输送量有显著影响,随着总压差的增加,煤粉输送量呈线性增加.研究结果对高压超浓相气力输送系统的设计、运行和控制具有指导作用.