排序方式: 共有95条查询结果,搜索用时 15 毫秒
61.
Parkes RJ Webster G Cragg BA Weightman AJ Newberry CJ Ferdelman TG Kallmeyer J Jørgensen BB Aiello IW Fry JC 《Nature》2005,436(7049):390-394
The sub-seafloor biosphere is the largest prokaryotic habitat on Earth but also a habitat with the lowest metabolic rates. Modelled activity rates are very low, indicating that most prokaryotes may be inactive or have extraordinarily slow metabolism. Here we present results from two Pacific Ocean sites, margin and open ocean, both of which have deep, subsurface stimulation of prokaryotic processes associated with geochemical and/or sedimentary interfaces. At 90 m depth in the margin site, stimulation was such that prokaryote numbers were higher (about 13-fold) and activity rates higher than or similar to near-surface values. Analysis of high-molecular-mass DNA confirmed the presence of viable prokaryotes and showed changes in biodiversity with depth that were coupled to geochemistry, including a marked community change at the 90-m interface. At the open ocean site, increases in numbers of prokaryotes at depth were more restricted but also corresponded to increased activity; however, this time they were associated with repeating layers of diatom-rich sediments (about 9 Myr old). These results show that deep sedimentary prokaryotes can have high activity, have changing diversity associated with interfaces and are active over geological timescales. 相似文献
62.
Nucleolar proteome dynamics 总被引:2,自引:0,他引:2
The nucleolus is a key organelle that coordinates the synthesis and assembly of ribosomal subunits and forms in the nucleus around the repeated ribosomal gene clusters. Because the production of ribosomes is a major metabolic activity, the function of the nucleolus is tightly linked to cell growth and proliferation, and recent data suggest that the nucleolus also plays an important role in cell-cycle regulation, senescence and stress responses. Here, using mass-spectrometry-based organellar proteomics and stable isotope labelling, we perform a quantitative analysis of the proteome of human nucleoli. In vivo fluorescent imaging techniques are directly compared to endogenous protein changes measured by proteomics. We characterize the flux of 489 endogenous nucleolar proteins in response to three different metabolic inhibitors that each affect nucleolar morphology. Proteins that are stably associated, such as RNA polymerase I subunits and small nuclear ribonucleoprotein particle complexes, exit from or accumulate in the nucleolus with similar kinetics, whereas protein components of the large and small ribosomal subunits leave the nucleolus with markedly different kinetics. The data establish a quantitative proteomic approach for the temporal characterization of protein flux through cellular organelles and demonstrate that the nucleolar proteome changes significantly over time in response to changes in cellular growth conditions. 相似文献
63.
During development, information about the three-dimensional shape and mechanical properties of the body is laid down in the synaptic connectivity of sensorimotor systems through unknown adaptive mechanisms. In spinal reflex systems, this enables the fast transformation of complex sensory information into adequate correction of movements. Here we use a computer simulation to show that an unsupervised correlation-based learning mechanism, using spontaneous muscle twitches, can account for the functional adaptation of the withdrawal reflex system. We also show that tactile feedback resulting from spontaneous muscle twitches during sleep does indeed modify sensorimotor transformation in young rats in a predictable manner. The results indicate that these twitches, corresponding to human fetal movements, are important in spinal self-organization. 相似文献
64.
Stracke S Kistner C Yoshida S Mulder L Sato S Kaneko T Tabata S Sandal N Stougaard J Szczyglowski K Parniske M 《Nature》2002,417(6892):959-962
Most higher plant species can enter a root symbiosis with arbuscular mycorrhizal fungi, in which plant carbon is traded for fungal phosphate. This is an ancient symbiosis, which has been detected in fossils of early land plants. In contrast, the nitrogen-fixing root nodule symbioses of plants with bacteria evolved more recently, and are phylogenetically restricted to the rosid I clade of plants. Both symbioses rely on partially overlapping genetic programmes. We have identified the molecular basis for this convergence by cloning orthologous SYMRK ('symbiosis receptor-like kinase') genes from Lotus and pea, which are required for both fungal and bacterial recognition. SYMRK is predicted to have a signal peptide, an extracellular domain comprising leucine-rich repeats, a transmembrane and an intracellular protein kinase domain. Lotus SYMRK is required for a symbiotic signal transduction pathway leading from the perception of microbial signal molecules to rapid symbiosis-related gene activation. The perception of symbiotic fungi and bacteria is mediated by at least one common signalling component, which could have been recruited during the evolution of root nodule symbioses from the already existing arbuscular mycorrhiza symbiosis. 相似文献
65.
Lasonder E Ishihama Y Andersen JS Vermunt AM Pain A Sauerwein RW Eling WM Hall N Waters AP Stunnenberg HG Mann M 《Nature》2002,419(6906):537-542
The annotated genomes of organisms define a 'blueprint' of their possible gene products. Post-genome analyses attempt to confirm and modify the annotation and impose a sense of the spatial, temporal and developmental usage of genetic information by the organism. Here we describe a large-scale, high-accuracy (average deviation less than 0.02 Da at 1,000 Da) mass spectrometric proteome analysis of selected stages of the human malaria parasite Plasmodium falciparum. The analysis revealed 1,289 proteins of which 714 proteins were identified in asexual blood stages, 931 in gametocytes and 645 in gametes. The last two groups provide insights into the biology of the sexual stages of the parasite, and include conserved, stage-specific, secreted and membrane-associated proteins. A subset of these proteins contain domains that indicate a role in cell-cell interactions, and therefore can be evaluated as potential components of a malaria vaccine formulation. We also report a set of peptides with significant matches in the parasite genome but not in the protein set predicted by computational methods. 相似文献
66.
Church C Moir L McMurray F Girard C Banks GT Teboul L Wells S Brüning JC Nolan PM Ashcroft FM Cox RD 《Nature genetics》2010,42(12):1086-1092
Genome-wide association studies have identified SNPs within FTO, the human fat mass and obesity-associated gene, that are strongly associated with obesity. Individuals homozygous for the at-risk rs9939609 A allele weigh, on average, ~3 kg more than individuals with the low-risk T allele. Mice that lack FTO function and/or Fto expression display increased energy expenditure and a lean phenotype. We show here that ubiquitous overexpression of Fto leads to a dose-dependent increase in body and fat mass, irrespective of whether mice are fed a standard or a high-fat diet. Our results suggest that increased body mass results primarily from increased food intake. Mice with increased Fto expression on a high-fat diet develop glucose intolerance. This study provides the first direct evidence that increased Fto expression causes obesity in mice. 相似文献
67.
Bis JC Kavousi M Franceschini N Isaacs A Abecasis GR Schminke U Post WS Smith AV Cupples LA Markus HS Schmidt R Huffman JE Lehtimäki T Baumert J Münzel T Heckbert SR Dehghan A North K Oostra B Bevan S Stoegerer EM Hayward C Raitakari O Meisinger C Schillert A Sanna S Völzke H Cheng YC Thorsson B Fox CS Rice K Rivadeneira F Nambi V Halperin E Petrovic KE Peltonen L Wichmann HE Schnabel RB Dörr M Parsa A Aspelund T Demissie S Kathiresan S Reilly MP Taylor K Uitterlinden A Couper DJ Sitzer M 《Nature genetics》2011,43(10):940-947
Carotid intima media thickness (cIMT) and plaque determined by ultrasonography are established measures of subclinical atherosclerosis that each predicts future cardiovascular disease events. We conducted a meta-analysis of genome-wide association data in 31,211 participants of European ancestry from nine large studies in the setting of the Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) Consortium. We then sought additional evidence to support our findings among 11,273 individuals using data from seven additional studies. In the combined meta-analysis, we identified three genomic regions associated with common carotid intima media thickness and two different regions associated with the presence of carotid plaque (P < 5 × 10(-8)). The associated SNPs mapped in or near genes related to cellular signaling, lipid metabolism and blood pressure homeostasis, and two of the regions were associated with coronary artery disease (P < 0.006) in the Coronary Artery Disease Genome-Wide Replication and Meta-Analysis (CARDIoGRAM) consortium. Our findings may provide new insight into pathways leading to subclinical atherosclerosis and subsequent cardiovascular events. 相似文献
68.
Formalised and Non-Formalised Methods in Resource Management—Knowledge and Social Learning in Participatory Processes: An Introduction 总被引:1,自引:1,他引:0
Jens Newig Helmut Haberl Claudia Pahl-Wostl Dale S. Rothman 《Systemic Practice and Action Research》2008,21(6):381-387
The participation of non-state actors in public decision-making and transdisciplinary research is increasingly regarded as
an effective means to cope with growing uncertainties and complexities in human–nature interactions. The management of natural
resources is expected to profit from a broader knowledge base and processes of social learning, thus allowing for potentially
more informed and creative decision-making. Communication is a key element of transmitting knowledge and fostering social
learning. This article introduces the special issue, which assembles contributions that discuss different methods, instruments,
tools, and models that have been developed in order to facilitate the transmission of information as well its selection and
aggregation. Each of the contributions is briefly reviewed. The approaches discussed here and in the individual papers aim
to foster learning in participatory processes. We argue that a key aspect is the degree to which methods are formalised. Formalisation
refers to the extent to which information is channelled in a certain way, leaving more or less scope for open communication.
Depending on the goals and context, more or less formalised methods can be employed. We conclude by highlighting the context-dependency
of participatory processes in natural resource management and indicate some directions for future research.
相似文献
Jens NewigEmail: |
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70.
Rolles D Braune M Cvejanović S Gessner O Hentges R Korica S Langer B Lischke T Prümper G Reinköster A Viefhaus J Zimmermann B McKoy V Becker U 《Nature》2005,437(7059):711-715
Because of inversion symmetry and particle exchange, all constituents of homonuclear diatomic molecules are in a quantum mechanically non-local coherent state; this includes the nuclei and deep-lying core electrons. Hence, the molecular photoemission can be regarded as a natural double-slit experiment: coherent electron emission originates from two identical sites, and should give rise to characteristic interference patterns. However, the quantum coherence is obscured if the two possible symmetry states of the electronic wavefunction ('gerade' and 'ungerade') are degenerate; the sum of the two exactly resembles the distinguishable, incoherent emission from two localized core sites. Here we observe the coherence of core electrons in N(2) through a direct measurement of the interference exhibited in their emission. We also explore the gradual transition to a symmetry-broken system of localized electrons by comparing different isotope-substituted species--a phenomenon analogous to the acquisition of partial 'which-way' information in macroscopic double-slit experiments. 相似文献