Membrane sorption property effects on transmembrane permeation

A theoretical study was carried out into membrane transport phenomena.Formulae for calculating the membrane transport resistance and transmembrane mass flux were given,variations in membrane resistance and moisture flux with the membrane sorption constant(C)under various humidity conditions were analyzed,and the value of C corresponding to the minimum membrane resistance or the maximum moisture flux was obtained.The results show that the membrane resistance and moisture flux relate not only to C but also to the relative humidities on both sides of the membrane.As C increases,membrane resistance initially decreases but then increases,i.e.,a minimum occurs,while the moisture flux first decreases and then increases,i.e.,a maximum occurs.The membrane resistance and moisture flux reach their extrema at the same value of C,which is determined by the relative humidities on both sides of the membrane.To reduce the membrane resistance,the value of C should be chosen based on the humidity conditions.     

Reverberation nulling and echo enhancement at low frequency using waveguide invariance

Based on the fact that the transfer function vector between a source receiver array and the dominant scatterer of boundary reverberation at a range can be obtained from the corresponding reverberations scattered from this range cell, a reverberation nulling concept using time reversal processing has been proposed. However, current reverberation nulling methods have certain limitations when applied into practice, which would null boundary reverberation and target echo simultaneously. As a solution, a passive reverberation nulling and echo enhancement method at low frequency using waveguide invariance is proposed in this paper. In this method, the reverberation subspace for the target range cell is not obtained directly from the return signals scattered by the target range cell but from the return signals scattered by a range cell located before the target using waveguide invariance, so as to suppress the reverberation embodied in the target echo by passive reverberation nulling. Besides, a range-dependent optimal weighting vector rather than conventional projector matrix is deduced to null the reverberation component meanwhile maximizing the target echo, thereby enhancing the echo-to-reverberation ratio furthest. Numerical simulations in typical range-independent shallow water environment demonstrate the efficacy and the improved performance of the proposed method for echo-to-reverberation enhancement.     

The effects of the guide field on the structures of electron density depletions in collisionless magnetic reconnection

Two-dimensional particle-in-cell simulations are performed to investigate the formation of electron density depletions in collisionless magnetic reconnection.In anti-parallel reconnection,the quadrupole structures of the out-of-plane magnetic field are formed,and four symmetric electron density depletion layers can be found along the separatrices due to the effects of magetic mirror.With the increase of the initial guide field,the symmetry of both the out-of-plane magnetic field and electron density depletion layers is distorted.When the initial guide field is sufficiently large,the electron density depletion layers along the lower left and upper right separatrices disappear.The parallel electric field in guide field reconnection is found to play an important role in forming such structures of the electron density depletion layers.The structures of the out-of-plane magnetic field By and electron depletion layers in anti-parallel and guide field reconnection are found to be related to electron flow or in-plane currents in the separatrix regions.In anti-parallel reconnection,electrons flow towards the X line along the separatrices,and are directed away from the X line along the magnetic field lines just inside the separatrices.In guide field reconnection,electrons can only flow towards the X line along the upper left and lower right separatrices due to the existence of the parallel electric field in these regions.     

考虑地质分层约束的长短期记忆循环神经网络测井曲线重构

延长油田东部裸眼井区早期测井资料普遍只有自然电位（SP）、自然伽马（GR）及梯度电阻率(R2.5)三条曲线,因缺失声波（AC）、地层电阻率（RT）等测井曲线,难以满足精细油藏地质研究需求。东部裸眼井区开发时间长、单井产量低,重新测井缺乏可行性及经济性。采用长短期记忆循环神经网络(Long Short-Term Memory,LSTM)进行缺失测井曲线重构是一种经济有效方法,适用于地层测井序列数据。然而延长油田东部浅层油藏上覆黄土层段测井数据信号干扰大,直接应用模型精度较差。针对此问题,本文采用考虑地质分层约束的LSTM模型进行缺失测井曲线的重构,通过分层数据截取每口井长6层段测井数据作为样本数据,既保留了LSTM模型处理序列数据的优势,同时又避免了上覆黄土层测井数据对模型的干扰。利用裸眼井区完整测井数据进行模型训练优化和验证,讨论了考虑地质分层约束的LSTM测井曲线重构精度,结果表明通过引入地质分层约束,模型重构测井曲线精度更高。应用优化后模型实现裸眼井区50口仅有GR、SP、R2.5三条曲线数据井的AC、RT曲线重构,对50口井的142个射孔段进行二次解释,对比试油解释结论符合率达到89.4%,验证了该方法对测井曲线重构的实用性和有效性。     

First observation of mesospheric and thermospheric winds by a Fabry-Perot interferometer in China

A Fabry-Perot interferometer, funded by the Meridian Project in China, was deployed at the Xinglong station (40.2°N,117.4°E) of the National Astronomical Observatories in Hebei Province, China. The instrument has been operating since April 2010, measuring mesospheric and thermospheric winds. The first observational data of winds at three heights in the mesosphere and thermosphere were analyzed, demonstrating the capacity of this instrument to aid basic scientific research. The wavelengths of three airglow emissions were OH892.0, OI 557.7, and OI 630.0 nm, which corresponded to heights of 87, 98, and 250 km, respectively. Three 38-day data sets of horizontal winds, from April 5, 2010 to May 12, 2010, show clear day-to-day variations at the same height. The minimum and maximum meridional winds at heights of 87, 98, and 250 km were –16.5 to 8.7 m/s, –24.4 to 15.9 m/s, and –43.6 to 1.5 m/s. Measurements of zonal winds were –5.4 to 7.6 m/s, 2.3 to 23.0 m/s, and –22.6 to 49.3 m/s. The average data from the observations was consistent with the data from HWM93. The wind data at heights of 87 and 98 km suggest a semi-diurnal oscillation, clearly consistent with HWM93 results. Conversely there was a clear discrepancy between the observations and the model at 250 km. In general, this Fabry-Perot interferometer is a useful ground-based instrument for measuring mesospheric and thermospheric winds at middle latitudes.     

Importance of NPA motifs in the expression and function of water channel aquaporin-1

The asparagine-proline-alanine sequences (NPA motifs) are highly conserved in aquaporin water channel family. Crystallographic studies of AQP1 structure demonstrated that the two NPA motifs are in the narrow central constriction of the channel, serving to bind water molecules for selective and effi-cient water passage. To investigate the importance of the two NPA motifs in the structure, function and biogenesis of aquaporin water channels, we generated AQP1 mutations with NPA1 deletion, NPA2 de-letion and NPA1,2 double deletion. The coding sequences of the three mutated cDNAs were subcloned into the mammalian expression vector pcDNA3.1 to form expression plasmids. We established stably transfected CHO cell lines expressing these AQP1 mutants. Immunofluorescence indicated that all the three mutated AQP1 proteins are expressed normally on the plasma membrane of stably transfected CHO cells, suggesting that deletion of NPA motifs does not influence the expression and intracellular processing of AQP1. Functional analysis demonstrated that NPA1 or NPA2 deletion reduced AQP1 water permeability by 49.6% and 46.7%, respectively, while NPA1,2 double deletion had little effect on AQP1 water permeability. These results provide evidence that NPA motifs are important for water per-meation but not essential for the expression, intracellular processing and the basic structure of AQP1 water channel.     

Sequence similarity analysis of non-self CTL epitopes and mouse proteins using sequence alignment

This research analyzed amino acid sequence similarity between non-self T cell epitopes recognized by mouse antibodies and mouse proteins. Using sequence alignment,we found that only 8 of 1 108 epitopes are highly similar to mouse protein sequences. The result shows that non-self T cell epitopes are not similar or have little similarity to mouse protein sequences. Furthermore,reviewing the related literature,we also found that the eight epitopes would trigger immune responses in some particular environment,which are ignored by T cells in normal condition. The result suggests that no or low-similarity peptide vaccines can reduce the chance of collateral cross-reactions and enhance the antigen-specific immune response to vaccine.     

Photocatalytic activity of TiO<Subscript>2</Subscript> sensitized by CdS quantum dots under visible-light irradiation

In this paper, CdS quantum dots sensitized TiO2 composite powders (QD-CdS/TiO2) were synthesized by impregnating TiO2 powder into CdS sol with different concentrations. X-ray diffraction pattern (XRD) shows the crystal structures of CdS and TiO2 are cubic phase and anatase phase separately in QD- CdS/TiO2 powder samples; the crystal size of CdS in QD- CdS/TiO2 is about 3-7 nm, while TiO2 crystal size is about 20 nm. With increasing CdS content in QD-CdS/TiO2 composite, the UV-Vis absorption spectrum shifted to the longer wavelength lines, exhibiting obvious quantum size effect. The fluorescence intensity of QD-CdS/TiO2 irradiated by blue light is weaker than that of pure CdS. When the molar ratio of CdS and TiO2 is about 1∶2, the QD-CdS/TiO2 powder has the best catalytic properties under visible-light irradiation, and the degradation rate of rhodamine B (RhB) is up to 92.2% within 60 min.