Cloning of GF14 Gene from the Bulbs of Fritillaria crrishoa

GF14 widely occurs in plant species functioning as a regulatory protein in cell signal transdution,including monoamine synthesis,exocytosis and cell cycle regulation.A partial sequence,413bp in length,is cloned from the bulbs of F.crrihosa by means of RT-CPR technique.Sequence alignment with the BLAST program shows that it is highly similar to GF14 gene.A new full-length cDNA,1133bp,is obtained by RACE techniques,It contains a major open reading frame of 262 amino acids,5‘ and 3‘untranslated regions and a long Poly(A) tail,Sequence analysis of deduced GF14 protein from F.crrihosa indicates several conserved motifs of GF14 protiens are also found in sequence of GF14 protein of F.crrihosa.     

A Combination Method of SVD And Neuro－Controller for Identifying Uncertain Systems

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A note on Whitney''''s critical sets

Abstract The critical arc is an arc which is the critical set for some differentiable function not constant along the arc. In literatureon the topic, the differentiable functions are always increasing along the arc, but in this paper we provide a critical arc such that the corre-sponding differentiable function could not be increasing along the arc. It is also proved that any self-similar Cantor set of dimension greaterthan 1 is a generalized Whitney's critical set.     

Human contribution to more-intense precipitation extremes

Extremes of weather and climate can have devastating effects on human society and the environment. Understanding past changes in the characteristics of such events, including recent increases in the intensity of heavy precipitation events over a large part of the Northern Hemisphere land area, is critical for reliable projections of future changes. Given that atmospheric water-holding capacity is expected to increase roughly exponentially with temperature--and that atmospheric water content is increasing in accord with this theoretical expectation--it has been suggested that human-influenced global warming may be partly responsible for increases in heavy precipitation. Because of the limited availability of daily observations, however, most previous studies have examined only the potential detectability of changes in extreme precipitation through model-model comparisons. Here we show that human-induced increases in greenhouse gases have contributed to the observed intensification of heavy precipitation events found over approximately two-thirds of data-covered parts of Northern Hemisphere land areas. These results are based on a comparison of observed and multi-model simulated changes in extreme precipitation over the latter half of the twentieth century analysed with an optimal fingerprinting technique. Changes in extreme precipitation projected by models, and thus the impacts of future changes in extreme precipitation, may be underestimated because models seem to underestimate the observed increase in heavy precipitation with warming.     

Efficient and selective recovery of Ni,Cu, and Co from low-nickel matte via a hydrometallurgical process

Low-nickel matte was intensively characterized, and Ni, Cu, and Co were determined to exist mainly as (Fe,Ni)₉S₈ and FeNi₃, Cu₅FeS₄, and (Fe,Ni)₉S₈ and Fe₃O₄ (in isomorphic form), respectively. The efficient and selective extraction of Ni, Cu, and Co from the low-nickel matte in an (NH₄)₂S₂O₈/NH₃·H₂O solution system was studied. The effects of (NH₄)₂S₂O₈ and NH₃·H₂O concentrations, leaching time, and leaching temperature on the metal extraction efficiency were systematically investigated. During the oxidative ammonia leaching process, the metal extraction efficiencies of Ni 81.07%, Cu 93.81%, and Co 71.74% were obtained under the optimal conditions. The relatively low leaching efficiency of Ni was mainly ascribed to NiFe alloy deactivation in ammonia solution. By introducing an acid pre-leaching process into the oxidative ammonia leaching process, we achieved the high extraction efficiencies of 98.03%, 99.13%, and 85.60% for the valuable metals Ni, Cu, and Co, respectively, from the low-nickel matte.     

Cleanliness of Ti-bearing Al-killed ultra-low-carbon steel during different heating processes

During the production of Ti-bearing Al-killed ultra-low-carbon (ULC) steel, two different heating processes were used when the converter tapping temperature or the molten steel temperature in the Ruhrstahl-Heraeus (RH) process was low:heating by Al addition during the RH decarburization process and final deoxidation at the end of the RH decarburization process (process-I), and increasing the oxygen content at the end of RH decarburization, heating and final deoxidation by one-time Al addition (process-Ⅱ). Temperature increases of 10℃ by different processes were studied; the results showed that the two heating processes could achieve the same heating effect. The T.[O] content in the slab and the refining process was better controlled by process-I than by process-Ⅱ. Statistical analysis of inclusions showed that the numbers of inclusions in the slab obtained by process-I were substantially less than those in the slab obtained by process-Ⅱ. For process-I, the Al₂O₃ inclusions produced by Al added to induce heating were substantially removed at the end of decarburization. The amounts of inclusions were substantially greater for process-Ⅱ than for process-I at different refining stages because of the higher dissolved oxygen concentration in process-Ⅱ. Industrial test results showed that process-I was more beneficial for improving the cleanliness of molten steel.     

Tempering stability of Fe-Cr-Mo-W-V hot forging die steels

The tempering stability of three Fe-Cr-Mo-W-V hot forging die steels (DM, H21, and H13) was investigated through hardness measurements and transmission electron microscopy (TEM) observations. Both dilatometer tests and TEM observations revealed that DM steel has a higher tempering stability than H21 and H13 steels because of its substantial amount of M₂C (M represents metallic element) carbide precipitations. The activation energies of the M₂C carbide precipitation processes in DM, H21, and H13 steels are 236.4, 212.0, and 228.9 kJ/mol, respectively. Furthermore, the results indicated that vanadium atoms both increase the activation energy and affect the evolution of M₂C carbides, resulting in gradual dissolution rather than over-aging during tempering.     

Functional coefficient autoregressive conditional root model

This paper proposes an extended model based on ACR model:Functional coefficient autoregressive conditional root model（FCACR）.Under some assumptions,the authors show that the process is geometrically ergodic,stationary and all moments of the process exist.The authors use the polynomial spline function to approximate the functional coefficient,and show that the estimate is consistent with the rate of convergence O_p(h^v+1+n^-1/3).By simulation study,the authors discover the proposed method can approximate well the real model.Furthermore,the authors apply the model to real exchange rate data analysis.     

Extraction and separation of nickel and cobalt from saprolite laterite ore by microwave-assisted hydrothermal leaching and chemical deposition

Extraction and separation of nickel and cobalt from saprolite laterite ore were studied by using a method of microwave-assisted hydrothermal leaching and chemical deposition. The effects of leaching temperature and time on the extraction efficiencies of Ni2+ and Co2+ were investigated in detail under microwave conditions. It is shown that the extraction efficiencies of Ni2+ and Co2+ from the ore pre-roasted at 300℃ for 5 h were 89.19% and 61.89% when the leaching temperature and time were about 70℃ and 60 min, respectively. For the separation process of Ni and Co, the separation of main chemical components was performed by adjusting the pH values of sulfuric leaching solutions using a NaOH solution based on the different pH values of precipitation for metal hydroxides. The final separation efficiencies of Ni and Co were 77.29% and 65.87%, respectively. Furthermore, the separation efficiencies of Fe of 95.36% and Mg of 92.2% were also achieved at the same time.