Estimating Fatty Acid Composition of Infant Buccal Mucosal Cells by Capillary Gas Chromatography

Long chain polyunsaturated fatty adds, i. e., docosahexaenoic acid （DHA or C22 ： 6n - 3）, amchidonic acid （AA or C20 ： 4n -6） have been identified as essential fatty acids and play an important role in growth and development of infants. Measurement of fatty acid composition is usually by collection of blood, but to obtain blood in infants is difficult. Nowadays, the fatty acid composition can be estimated by collecting buccal mucosal cells, which can avoid repeated blood sampling. The of this paper is to compare the fatty acid composition of cheek cells with that of plasma and red blood cells （RBCs）. In this study, twenty-seven infants were enrolled, and buccal mucosal cells and blood samples were obtained from these infants of the same time. Fatty acid composition of buccal mucosal cells, plasma and RBCs were measured by capillary gas chromatography. The results show that the contents of AA and DHA in the buccal macosal cells are correlated well with that in the plasma [r=0.36 （P=0.042） and r =0.38 （P =0.033）, respectively]. The ratio of AA to DHA is 1.32% in buccal mucosal cells, 1.60% in plasma and 1.55% in RBCs and there are no significant differences among groups （P = 0.134）. It shows that the fatty acid composition in buccal macosal cells can reflect the fat nutrition status in infants and can be detected by capillary gas chromatography. Estimating fatty acid composition of buceal mucosal cells in infants by capillary gas chromatography is feasible, and because of its noninvasiveness, it can be suitable for nutrition research in infants.     

A New Method of Color Edge Detection Based on Local Structure Analysis

Human＇s real life is within a colorful world. Compared to the gray images, color images contain more information and have better visual effects. In today＇s digital image processing, image segmentation is an important section for computers to ＂understand＂ images and edge detection is always one of the most important methods in the field of image segmentation. Edges in color images are considered as local discontinuities both in color and spatial domains. Despite the intensive study based on integration of single-channel edge detection results, and on vector space analysis, edge detection in color images remains as a challenging issue.     

基于途径分析的L-苏氨酸发酵过程优化

以L-苏氨酸生产菌株TRFC为供试菌株,在拟稳态下基于途径分析对发酵过程作出理论分析,并对发酵过程进行优化.通过途径分析方法确定L-苏氨酸合成代谢途径的11种基本模型,其中模型1、3、4、6、9、11最高理论产率为86%.根据途径分析结果,提出L-苏氨酸产生菌的发酵控制策略,并进行摇瓶及发酵罐实验验证.结果表明:在添加葡萄糖酸钠发酵过程中,L-苏氨酸产量与葡萄糖为唯一碳源时相比反而降低;且充分供氧达20%时,代谢流大量涌入目的产物,L-苏氨酸产率最大.     

高寒地区混凝土重力坝温控防裂研究

针对雅鲁藏布江中游段特殊的高原气候条件,根据重力坝的施工进度计划,对混凝土重力坝施工期和运行期的温度场、应力场进行了动态的仿真计算.研究了在不同季节开始浇筑坝体混凝土时坝体约束区的应力场、施工期闸墩的应力场和冬季开始浇筑坝体的整体应力场;根据浇筑季节的不同、混凝土材料的不同、约束强弱的不同和经济实用的原则制定了相应的温控措施,保证坝体在施工期和运行期的安全.     

自来水业价格规制改革中存在问题与对策

价格规制改革是我国自来水业规制改革的核心内容,目前仍存在着沓价低、水资源费和污水处理费征收不到位、计费方式单一、定价程序欠规范等问题,需要从完善全成本覆盖价格体系和污水处理费的征收制度、改革自来水计费方式、规范自来水业定价程序等方面进行改革,以推动自来水业规制改革健康、有序进行.     

中职语文课堂教学与信息技术的整合

语文是职业中学的基础学科,由于学生的学业主体是技术实践课程,文化课基础大多比较薄弱,尝试在语文课堂教学中融入信息技术,激发学生的主体参与意识,提升学习兴趣,取得理想的语文教学效果.     

具有两种不同服务的M/G（MM）/1可修重试排队系统

考虑有两种不同服务的M/G(M/M)/1可修重试排队系统,假定此系统只有队首的顾客允许重试,服务台可为顾客提两种服务,每个顾客在接受完服务台提供的第一种服务后,要么以概率θ继续接受第二种服务,要么以概率1-θ进入重试区域,并且服务台在服务过程中可能损坏,通过补充变量法得到系统的队长和可靠性指标.     

研究型大学的创新人才培养机制探讨——南京大学研究生创新教育的实践与探索

分析目前影响我国研究生创新教育的相关因素,以南京大学为例,对研究型大学创新人才培养的机制做些探讨。     

一种轻量化网络的火焰烟雾检测算法

针对算力有限的移动和嵌入式平台,提出了一种基于深度学习的轻量化火焰烟雾检测算法.利用数据增强来解决数据量较少的问题,使用one-stage目标检测方法中的YOLOv4作为火焰烟雾检测的模型框架,采用轻量化神经网络MobileNetV3替换YOLOv4的原主干特征提取网络,减少了模型参数量;再采用深度可分离卷积替换掉YO...     

Msx1在小鼠腭板发育中的作用及机制研究

哺乳动物腭板发育受到多个信号通路以及转录因子的精密调控,参与该过程的基因突变常常会导致腭裂.Msx1是腭板发育调控的关键基因,其突变与腭裂密切相关.本研究利用条件性Msx1过表达小鼠,采用HE染色分析Msx1过表达小鼠腭板的表型,采用免疫组化研究Msx1过表达小鼠腭板的细胞增殖和腭板骨分化情况,利用RNA-seq测序分...