RLC串联谐振实验的直观教学法

传统的RLC串联谐振实验方法需用晶体管毫伏表大量测量数据，实验方法繁琐且没有直观性，在实验过程中不便于分析和讨论。本介绍一种新型实验方法，该方法可以克服上述不足之处，对学习掌握这部分内容起到了很好的作用。     

β葡聚糖酶杂合基因bglHAM的克隆及在P.pastoris中的表达

采用PCR的方法,以Bacillus macerans总DNA为模板,构建出β-1,3-1,4葡聚糖酶杂合基因bglHAM,与巴斯德毕赤酵母表达载体pPIC9K重组,得到重组质粒pPIC9K-HAM,电脉冲转化法转化酵母菌GS115,KM71,在MM,MD平板上筛选表型,YPD-G418平板上筛选多拷贝重组子,重组菌株经甲醇诱导后,刚果红平板染色法检测到β-葡聚糖酶活性,SDS-PAGE证明表达产物的分子量约为24kD,摇瓶诱导培养筛选到的重组菌株,胞外每mL发酵液最高酶活达240U。     

移动通信Rician信道中的多普勒影响分析

在研究分析了陆地移动通信Rician多径信道中因存在较强支配路径信号入射以及入射路径空间位置发生变化对Doppler频谱密度所产生影响的基础上 ,指出在实际无线传播环境中的一般性Doppler频谱密度通常应为非对称的 ,并且给出了相应的理论分析和仿真结果     

The mosaic that is our genome

The discovery of the basis of genetic variation has opened inroads to understanding our history as a species. It has revealed the remarkable genetic similarity we share with other individuals as well as with our closest primate relatives. To understand what make us unique, both as individuals and as a species, we need to consider the genome as a mosaic of discrete segments, each with its own unique history and relatedness to different contemporary and ancestral individuals.     

Targeting of the Akt/PKB kinase to the actin skeleton

Serine/threonine kinase Akt/PKB intracellular distribution undergoes rapid changes in response to agonists such as Platelet-derived growth factor (PDGF) or Insulin-like growth factor (IGF). The concept has recently emerged that Akt subcellular movements are facilitated by interaction with nonsubstrate ligands. Here we show that Akt is bound to the actin skeleton in in situ cytoskeletal matrix preparations from PDGF-treated Saos2 cells, suggesting an interaction between the two proteins. Indeed, by immunoprecipitation and subcellular fractioning, we demonstrate that endogenous Akt and actin physically interact. Using recombinant proteins in in vitro binding and overlay assays, we further demonstrate that Akt interacts with actin directly. Expression of Akt mutants strongly indicates that the N-terminal PH domain of Akt mediates this interaction. More important, we show that the partition between actin bound and unbound Akt is not constant, but is modulated by growth factor stimulation. In fact, PDGF treatment of serum-starved cells triggers an increase in the amount of Akt associated with the actin skeleton, concomitant with an increase in Akt phosphorylation. Conversely, expression of an Akt mutant in which both Ser473 and Thr308 have been mutated to alanine completely abrogates PDGF-induced binding. The small GTPases Rac1 and Cdc42 seem to facilitate actin binding, possibly increasing Akt phosphorylation.Received 10 September 2003; accepted 25 September 2003     

商业银行构建"金融超市"路径初探

文章阐述了商业银行构建“金融超市”的成因、必要性,提出通过商业银行经营实体的微观金融创新和金融监管部门的金融制度创新互动来构建“金融超市”。     

暗管排降对小麦养 分、干物质、籽粒产量及品质的影响

着重探讨了南方不同生态类型圩区农田埋设塑料暗管对小麦地上部分矿质营养吸收、干物质累积和分配、产量及品质的影响。试验结果表明：农田埋设塑料暗管排水能显著提高麦株地上部分对Ｎ、Ｐ,Ｋ矿质营养元素的吸收,促进小麦各生育期尤其是拔节孕穗期地上部分干物质累积,小麦增产幅度达到２０．３％￣５３．３％,并能提高籽粒蛋白质和氨基酸含量,改善小麦籽粒的品质。     

经验派美学“审美无利害”思想与审美现代性

18世纪英国的经验派美学家最早从主体自身出发提出审美不涉及利害的思想。这一思想最早由夏夫兹博里提出,哈奇生、艾迪生、休谟、伯克等人继承了审美无关利害的思想。在英国经验派美学的影响下,康德将＂审美无利害＂确立为一种美学原则。英国经验派美学提出的＂审美无利害＂,体现了审美现代性的分化性特征。英国经验派美学家以此改变了单纯以德国理性派来研究美学的现代起源的观点,其地位和作用由此得到突显。     

在非极性溶剂中杂多阴离子—CO络合物的电子光谱

合成了五种取代型杂多化合物：「ＳｉＷ１１Ｏ３９Ｃｏ（Ｈ２Ｏ）」＾６－，「ＧｅＷ１１Ｏ３９Ｃｏ（Ｈ２Ｏ）」＾６－，「ＰＷ１１Ｏ３９Ｃｏ（Ｈ２Ｏ）」＾５－，「Ｐ２Ｗ１７Ｏ６１Ｃｏ（Ｈ２Ｏ）」＾８－，「ＰｗＯ１８Ｏ６８Ｃｏ４（Ｈ２Ｏ）２」＾１０－，通过等离子体、ＩＲ、ＵＶ、极谱、热谱等测试手段对产物进行了表征、并首次报道它们在非极性溶剂中与ＣＯ小分子的作用，实验结果表明，取代型杂多化合物与ＣＯ形成了ＨＰ     

大变形体积不变时横向变形系数变化规律研究

以实验结果为基础，根据体积不变条件的一般表达式，研究了横向变形系数随主伸长度Ｅｉ改变时的变化规律。研究结果表明：当保留体积不变一般表达式中三个主伸长率的的所有项次时，Ｅ１超出一定范围后，横向变形系数等于０．５的结论不具普遍性．一般来说，它将是变形状态及Ｅ１的非线性函数。