全文获取类型
收费全文 | 347篇 |
免费 | 1篇 |
国内免费 | 2篇 |
专业分类
系统科学 | 5篇 |
丛书文集 | 1篇 |
教育与普及 | 1篇 |
现状及发展 | 71篇 |
研究方法 | 34篇 |
综合类 | 226篇 |
自然研究 | 12篇 |
出版年
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 1篇 |
2015年 | 2篇 |
2014年 | 3篇 |
2013年 | 2篇 |
2012年 | 25篇 |
2011年 | 44篇 |
2010年 | 15篇 |
2009年 | 3篇 |
2008年 | 11篇 |
2007年 | 15篇 |
2006年 | 24篇 |
2005年 | 10篇 |
2004年 | 10篇 |
2003年 | 6篇 |
2002年 | 7篇 |
2001年 | 10篇 |
2000年 | 11篇 |
1999年 | 7篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1992年 | 11篇 |
1991年 | 4篇 |
1990年 | 7篇 |
1989年 | 6篇 |
1987年 | 10篇 |
1986年 | 5篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 9篇 |
1978年 | 4篇 |
1977年 | 1篇 |
1976年 | 5篇 |
1975年 | 2篇 |
1974年 | 4篇 |
1973年 | 7篇 |
1971年 | 4篇 |
1970年 | 9篇 |
1969年 | 3篇 |
1968年 | 5篇 |
1967年 | 7篇 |
1966年 | 1篇 |
1965年 | 5篇 |
排序方式: 共有350条查询结果,搜索用时 15 毫秒
1.
Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry 总被引:54,自引:0,他引:54
A library of 50,000 recombinants representative of the human X chromosome has been constructed. Human X chromosomes were physically separated using a fluorescence-activated cell sorter. The DNA was purified from the chromosomes, digested to completion with the restriction enzyme EcoRI and cloned into the phage lambda gtWES.lambda B. The X-derived nature of the recombinants was confirmed by hybridization to rodent/human cell line DNA containing only the human X chromosome. Such libraries will be particularly useful for the investigation of genetic diseases such as Duchenne muscular dystrophy, where the basic defect has not been elucidated, and of neoplasia, where several specific chromosomal anomalies, particularly for the leukaemias, have been identified. 相似文献
2.
二氧化碳减量技术包括捕获、封存及再利用技术,是目前国际上亟待发展的减量技术的研究状况.阐述了近年来台湾致力于研发各项减量技术的研究状况,希望通过能源计划将针对化学吸收法、物理吸附法、超重力旋转填充床技术、化学循环程序、CO2转化技术等对台湾未来CO2减量提出一整合性技术.除了基础减量技术的研发之外,国科会通过能源计划整合产学研各界资源,共同开发CO2补获及再利用技术,并建立示范工厂,成为台湾CO2减量的重点技术平台. 相似文献
3.
Eoin E. Kelly Conor P. Horgan Mary W. McCaffrey Paul Young 《Cellular and molecular life sciences : CMLS》2011,68(2):185-194
Long-term potentiation (LTP) defines persistent increases in neurotransmission strength at synapses that are triggered by
specific patterns of neuronal activity. LTP, the most widely accepted molecular model for learning, is best characterised
at glutamatergic synapses on dendritic spines. In this context, LTP involves increases in dendritic spine size and the insertion
of glutamate receptors into the post-synaptic spine membrane, which together boost post-synaptic responsiveness to neurotransmitters.
In dendrites, the material required for LTP is sourced from an organelle termed the endosomal-recycling compartment (ERC),
which is localised to the base of dendritic spines. When LTP is induced, material derived from the recycling compartment,
which contains α-amino-3-hydroxy-5-methyl-4-isoxazole propionate-type glutamate receptors (AMPARs), is mobilised into dendritic
spines feeding the increased need for receptors and membrane at the spine neck and head. In this review, we discuss the importance
of endosomal-recycling and the role of key proteins which control these processes in the context of LTP. 相似文献
4.
Renin was found in the submandibular glands of male Quackenbush mice in concentrations higher than has been reported for any tissue of any strain or species. However, no renin-like activity could e detected in glands from male and female Wistar rats using either pH 5.8 or 7.4 for assay and a radioimmunoassay specific for renin's reaction product, angiotensin I. Rabbit submandibular glands contained renin. 相似文献
5.
6.
Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer 总被引:1,自引:0,他引:1
7.
8.
Young DA Wright AP Roberts JL Warner RC Young NW Greenbaum JS Schroeder DM Holt JW Sugden DE Blankenship DD van Ommen TD Siegert MJ 《Nature》2011,474(7349):72-75
The first Cenozoic ice sheets initiated in Antarctica from the Gamburtsev Subglacial Mountains and other highlands as a result of rapid global cooling ~34 million years ago. In the subsequent 20 million years, at a time of declining atmospheric carbon dioxide concentrations and an evolving Antarctic circumpolar current, sedimentary sequence interpretation and numerical modelling suggest that cyclical periods of ice-sheet expansion to the continental margin, followed by retreat to the subglacial highlands, occurred up to thirty times. These fluctuations were paced by orbital changes and were a major influence on global sea levels. Ice-sheet models show that the nature of such oscillations is critically dependent on the pattern and extent of Antarctic topographic lowlands. Here we show that the basal topography of the Aurora Subglacial Basin of East Antarctica, at present overlain by 2-4.5?km of ice, is characterized by a series of well-defined topographic channels within a mountain block landscape. The identification of this fjord landscape, based on new data from ice-penetrating radar, provides an improved understanding of the topography of the Aurora Subglacial Basin and its surroundings, and reveals a complex surface sculpted by a succession of ice-sheet configurations substantially different from today's. At different stages during its fluctuations, the edge of the East Antarctic Ice Sheet lay pinned along the margins of the Aurora Subglacial Basin, the upland boundaries of which are currently above sea level and the deepest parts of which are more than 1?km below sea level. Although the timing of the channel incision remains uncertain, our results suggest that the fjord landscape was carved by at least two iceflow regimes of different scales and directions, each of which would have over-deepened existing topographic depressions, reversing valley floor slopes. 相似文献
9.
10.
Widespread anoxia in the ocean is frequently invoked as a primary driver of mass extinction as well as a long-term inhibitor of evolutionary radiation on early Earth. In recent biogeochemical studies it has been hypothesized that oxygen deficiency was widespread in subsurface water masses of later Cambrian oceans, possibly influencing evolutionary events during this time. Physical evidence of widespread anoxia in Cambrian oceans has remained elusive and thus its potential relationship to the palaeontological record remains largely unexplored. Here we present sulphur isotope records from six globally distributed stratigraphic sections of later Cambrian marine rocks (about 499 million years old). We find a positive sulphur isotope excursion in phase with the Steptoean Positive Carbon Isotope Excursion (SPICE), a large and rapid excursion in the marine carbon isotope record, which is thought to be indicative of a global carbon cycle perturbation. Numerical box modelling of the paired carbon sulphur isotope data indicates that these isotope shifts reflect transient increases in the burial of organic carbon and pyrite sulphur in sediments deposited under large-scale anoxic and sulphidic (euxinic) conditions. Independently, molybdenum abundances in a coeval black shale point convincingly to the transient spread of anoxia. These results identify the SPICE interval as the best characterized ocean anoxic event in the pre-Mesozoic ocean and an extreme example of oxygen deficiency in the later Cambrian ocean. Thus, a redox structure similar to those in Proterozoic oceans may have persisted or returned in the oceans of the early Phanerozoic eon. Indeed, the environmental challenges presented by widespread anoxia may have been a prevalent if not dominant influence on animal evolution in Cambrian oceans. 相似文献