排序方式: 共有2条查询结果,搜索用时 0 毫秒
1
1.
Ruchaud-Sparagano MH Westley BR May FE 《Cellular and molecular life sciences : CMLS》2004,61(15):1946-1954
The trefoil protein TFF1 is expressed principally in the superficial cells of the gastric mucosa. It is a
small protein and forms homo- and hetero-dimers via a disulphide bond through Cys58 which is located three amino
acids from the C terminus. TFF1 is co-expressed with the secreted mucin MUC5AC in superficial cells of the gastric
mucosa suggesting that it could be involved in the packaging or function of gastric mucus. We have previously
shown that TFF1 co-sediments with mucin glycoproteins on caesium chloride gradients. To extend this observation
we have now used gel filtration under physiological conditions, immunoprecipitation and Western transfer analysis
to characterise the interaction of TFF1 with gastric mucin glycoproteins. We show that TFF1 co-elutes with MUC5AC
but not MUC6 on gel filtration and that immunoprecipitation and Western transfer analysis confirms that TFF1
interacts with MUC5AC. We also demonstrate that the TFF1 dimer is the predominant molecular form bound to MUC5AC.
Salt and chelators of divalent cations such as EDTA and EGTA disrupted the TFF1- MUC5AC interaction and increased
the degradation of MUC5AC, whereas calcium increased the amount of TFF1 bound to MUC5AC. These data support the
contention that TFF1 is pivotal in the packaging and function of human gastric mucusa.Received 24 March 2004; received after revision 14 May 2004; accepted 7 June 2004 相似文献
2.
In the modeling of microsegregation, the partition coefficient is usually calculated using data from the equilibrium phase diagrams. The aim of this study was to experimentally and theoretically analyze the partition coefficient in binary aluminum-copper alloys. The samples were analyzed by differential thermal analysis (DTA), which were melted and quenched from different temperatures during solidification. The mass fraction and composition of phases were measured by image processing and scanning electron microscopy (SEM) equipped with an energy-dispersive X-ray spectroscopy (EDS) unit. These data were used to calculate as the experimental partition coefficients with four different methods. The experimental and equilibrium partition coefficients were used to model the concentration profile in the primary phase. The modeling results show that the profiles calculated by the experimental partition coefficients are more consistent with the experimental profiles, compared to those calculated using the equilibrium partition coefficients. 相似文献
1