Reduced fertility in male mice following treatment with niridazole

Zusammenfassung Nach einmaliger i.p.-Injetion von 700 mg/kg Niridazol wurden männiiche Mäuse wiederholt mit unbehandelten Weibchen wöchentlich über 2 Monate hinweg gepaart und die Ova gesammelt. Es konnte eine starke Herabsetzung der Befruchtungsraten 3–4 Wochen nach Paarung festgestellt werden, was die Annahme einer Wirkung auf die meiotische Teilung der Spermatozyten und Spermatiden nahelegt. Nach der 7. Woche trat eine Normalisierung der Fruchtbarkeitsrate ein.

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This work was supported by NIH Grants No. C-6516 and No. FR 05526 and by the Environmental Protection Agency Contract No. CPA-70-17.     

Increased survival time of leukemic mice following treatment with phytohemagglutinin

Résumé Des souris ayant subi un traitement journalier au PHA, entrepris 4 jours avant l'implantation de cellules leucémiques L1210 on survécu plus longtemps que celles qui furent traitées au sel. Le traitement journalier entrepris 24 h après l'inoculation de cellules leucémiques n'a pas eu d'effet sur la durée de survie. Par le traitement au PHA combiné à la 6-mercaptopurine, la durée de vie fut plus longue que par celui que l'on obtint avec d'autres agents employés isolément. Un mécanisme proposé pour l'effet antileucémique de PHA est discuté.

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This work was supported in part by Contract No. NIH-70-2001 from the Cancer Chemotherapy National Service Center, National Cancer Institute and by a grant from the Life Insurance Medical Research fund No. G-68-11.     

Spermatocyte selection during meiosis following mitomycin C treatment in mice

Summary In mice treated with mitomycin C, elimination of spermatocytes is observed during meiotic division, whereby an increase in number of the eliminated cells is closely related to an increase in the frequency of spermatocytes with chromosome aberrations at M-I.     

Cytogenetic analysis of bone marrow cells and spermatogonia of male mice after in vivo treatment with arsenic

Summary Male Swiss Albino mice were injected i.p. with an As₂O₃ solution (0; 4; 8 and 12 mg As/kg) and sacrificed 12, 24, 36 and 48 h after injection. Neither chromatid nor chromosome aberrations were observed in bone marrow cells and spermatogonia.Acknowledgments. We thank Mr L. De Langhe for skillfull technical help. We acknowledge this work was supported by a grant of the Ministery of Public Health.     

Osseal changes in mice following neonatal thymectomy

Zusammenfassung In neonatal thymektomierten Mäusen wurden nach 6–7 Wochen röntgenologisch sowie histologisch das «wasting syndrome» und schwere Knochenatrophien beobachtet. Der Mechanismus der beobachteten enchondralen Ossifikationsstörung wurde diskutiert.     

Post-weaning differential housing and testosterone secretion in male mice

Summary The effect of different densities of animals per cage on basal testosterone secretion and its response to some stimuli was studied in prepuberal male mice. Mice housed with 110 or 55 cm² of floor space per animal showed the same basal serum testosterone and hCG-induced testosterone release. Likewise, in a second experiment, the same basal serum testosterone and a similar response to acute noise stress were found in mice housed at 132, 66 or 22 cm² of floor space per animal. These results suggest that post-weaning crowding did not affect Leydig cell function in male mice.     

Histochemical and ultrastructural modifications of mice endometrium, vagina and pituitary following zeranol treatment

The histochemical and ultrastructural changes produced by zeranol treatment on the endometrium and the gonadotropic FSH cells of the adenohypophysis have been investigated in mice at the prepuberal and virginal stages. Modifications similar to those induced by estrogen treatment were observed. It is concluded that both estrogens and zeranol share the same activity on the tissues examined.     

Histochemical and ultrastructural modifications of mice endometrium,vagina and pituitary following zeranol treatment

Summary The histochemical and ultrastructural changes produced by zeranol treatment on the endometrium and the gonadotropic FSH cells of the adenohypophysis have been investigated in mice at the prepuberal and virginal stages. Modifications similar to those induced by estrogen treatment were observed. It is concluded that both estrogens and zeranol share the same activity on the tissues examined.     

Reduced collagen biosynthesis in oral mucosa of beige (Chediak-Higashi) mice

Oral mucosa of beige (Chediak-Higashi) mice had decreased levels of collagen synthesis and prolyl hydroxylase activity compared with normal animals. No significant difference was observed in non-collagen protein synthesis between the two groups. These results suggest that decreased collagen biosynthesis in oral tissues may be partially involved in the increased incidence of periodontal disease in the Chediak-Higashi syndrome.     

Effect of alkylating agents derived from diamines on fertility of the male mouse

Zusammenfassung Nachweis, dass einige alkylierende Substanzen, die von verschiedenen Diaminen erhalten wurden, die Spermatogenese männlicher Swiss-Webster-Mäuse hemmen. Eine davon verursachte achtwöchige Sterilität und scheint zur Kontrolle von Nagetieren besonders geeignet.

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This work was supported by Stanford Research Institute's Research and Development Program.     

Reduced collagen biosynthesis in oral mucosa of beige (Chediak-Higashi) mice

Summary Oral mucosa of beige (Chediak-Higashi) mice had decreased levels of collagen synthesis and prolyl hydroxylase activity compared with normal animals. No significant difference was observed in non-collagen protein synthesis between the two groups. These results suggest that decreased collagen biosynthesis in oral tissues may be partially involved in the increased incidence of periodontal disease in the Chediak-Higashi syndrome.     

Effect of a sesquiterpene fromAristolochia indica Linn. on fertility in female mice

Summary A sesquiterpene isolated from the roots ofAristolochia indica (Linn.) was found to exert 100% interceptive activity and 91.7% anti-implantation activity in mice at a single oral dose of 100mg/kg b. wt. No toxic effect was found at the dose levels used.The authors wish to thank Dr S. C. Pakrashi and his associate Mr P. P. Ghosh Dastidar of Medicinal Chemistry Department for isolating the compound for this study. Thanks are also due to Director-General of Indian Council of Medical Research for granting a Junior Research Fellowship to C.S.