Inhibitory effect of ouabain on in vitro and in vivo gastric acid secretion in the frog and the rat

The in vitro and in vivo effects of ouabain on gastric acid secretion in the frog and the rat, the 2 species known to have different sensitivity to ouabain, were studied. It was found that ouabain was a potent inhibitor of histamine-stimulated acid secretion in the isolated frog gastric mucosa. Ouabain administered i.v. at dose levels far below the lethal range also produced a marked and significant reduction of histamine-stimulated gastric acid secretion in the anesthetized frogs and rats. It is considered that the inhibitory effect of ouabain on acid secretion could be partly related to its specific antagonizing action on the Na+ -K+ -ATPase in the gastric mucosa.     

Inhibition in the rat of gastric acid secretion and cyclic AMP analogs accumulation in vitro by somatostatin

Summary Various somatostatin (S) analogs exhibited similar degree and similar, or shorter, duration of inhibition of basal gastric acid secretion as S in the unanesthetized rat and similar, or less, inhibition of the cyclic AMP accumulation induced by prostaglandin E₂ in the rat anterior pituitary in vitro. With the analogs examined, the gastrointestinal and pituitary receptors appear to exhibit generally similar recognition specificity with the differences within the gastrointestinal activities reflecting duration of availability rather than receptor affinity.The author acknowledges the technical assistance of Mr L. Chamberland, Miss A. Colaviti and Miss C. Pilapil.     

Inhibition in the rat of gastric acid secretion and cyclic AMP analogs accumulation in vitro by somatostatin.

Various somatostatin (S) analogs exhibited similar degree and similar, or shorter, duration of inhibition of basal gastric acid secretion as S in the unanesthetized rat and similar, or less, inhibition of the cyclic AMP accumulation induced by prostaglandin E2 in the rat anterior pituitary in vitro. With the analogs examined, the gastrointestinal and pituitary receptors appear to exhibit generally similar recognition specificity with the differences within the gastro-intestinal activities reflecting duration of availability rather than receptor affinity.     

Corticoid and salicylate induction of gastric acid secretion in the vagotomized rat

Zusammenfassung An der narkotisierten Ratte wurde bei submuköser Anwendung der Einfluss von acetylsalicylischer Säure und Hydrokortisonacetat auf die Sekretion von Salzsäure in normalen und vagotomierten Tieren untersucht. Beide Stoffe hatten bei allen Tieren eine anregende Wirkung auf die Absonderung von Magensäure, die durch lokale Anwendung von Atropin gehemmt werden konnte. Es scheint wahrscheinlich, dass der ulcerogene Effekt von Salicylaten und Kortizoiden zumindest teilweise auf die direkte Stimulation des intramuralen Nervenplexus zurückzuführen ist.

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This investigation was supported by a PHS research grant, No. A-5560, from the Division of Pharmacology and Experimental Therapeutics, N.I.H. (USA).     

Inhibitory action of cholecystokinin-pancreozymin on gastric pepsin secretion

Zusammenfassung Der Einfluss von Cholecystokinin-Pankrcozymin auf die Sekretion von Pepsin wurde in Hunden mit Heidenhain-Tasche untersucht. Während submaximaler Pepsinstimulation mit Mecholyl verminderte i.v. Injektion von Cholecystokinin-Pankreozymin die Pepsin-Sekretion im Magen signifikant. Es wird vermutet, dass CCK-PZ Enterogastrone sein könnte.     

Hydrochloric acid secretion of fetal rat gastric mucosa]

In Rat, spontaneous secretion of hydrochloric acid by gastric mucosa takes place before birth. From day 20 of gestation until birth, the pH of gastric content markedly decreases, reaching 3 pH-units in newborns. During this period, Cl- concentration in gastric juice increases, while p-CO2 remains constant.     

The effect of ouabain on water consumption in the rat

Résumé L'ouabain diminue la consommation d'eau, stimulée par l'injection d'une solution sallée hypertonique, chez les rats normaux ou néphrectomisés.     

Inhibitory influence of ligation of the small intestine on gastric secretion in the pylorus-ligated rats

Résumé La ligature de l'intestin, particulièrement du duodénum, gêne la sécrétion gastrique chez le Rat à pylore lié avec ou sans fistule fine.     

Chronic effects of nicotine on rat gastric secretion

Résumé Après avoir reÇu 300 g de tartrate de nicotinehydrogène pendant 15 jours il y eut des augmentations significatives dans le volume du suc gastrique et dans la production de pepsine. Des injections de nicotine on abaissé tous les paramètres, mais combinées avec de la gastrine synthétique (ICI-50123), elles n'ont aucun effet. Les résultats présentés peuvent expliquer pourquoi l'habitude de fumer est un facteur contribuant à la formation à la guérison des ulcères peptiques.     

Effect of deglycyrrhizinized liquorice on gastric acid secretion,histidine decarboxylase activity and serum gastrin level in the rat

Zusammenfassung Nachweis, dass die Behandlung mit einem deglycyrrhizinierten Lakrizpräparat die basale Säurereaktion bei Ratten hemmt. Erhöhtes antrales pH führt zu vermehrter Freisetzung von antralem Gastrin mit bedeutender Aktivierung der Histidindecarboxylase in der Magenschleimhaut.

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Acknowledgments: This study was supported by the Swedish Medical Research Council No. B73-04x-1007-08 and by the Albert Påhlsson Foundation.     

Inhibitory effect of taurine on decrease in the inotropic action of ouabain at high concentrations in isolated atria

Summary In vitro, taurine was shown to inhibit the decrease in the inotropic effect of ouabain at large doses in the normal and also low K⁺ medium in which this decrease in the inotropism of ouabain was facilitated. This inhibitory effect of taurine was, at least in part, due to the inhibition of the efflux of intracellular K⁺ in the isolated heart.     

The effect of propylthiouracil on glutathione S-transferase activity of rat spleen in vitro and in vivo

Propylthiouracil (PTU) inhibited glutathione (GSH) S-transferase (EC 2.5.1.18) activity of rat spleens in a concentration dependent manner in vitro. PTU (1.5 mmoles/kg) treatment of rats for 1 or 2 weeks caused a decrease in leukocyte number and spleen weight. Nevertheless, GSH S-transferase activity was not affected by the same treatment.     

The effect of hog gastrin on gastric secretion in chronic gastric fistula rats

Résumé L'action de la gastrine de porc a été étudiée chez des rats mâles munis de fistules gastriques. La gastrine était injectée par voie s.c. à des doses de 1–2 ml: 1 ml équivalant à l'activité de 10 g de muqueuse de porc. Le volume de suc gastrique, le volume de suc gastrique par 100 g, l'acidité totale et le débit d'acide accusèrent une pointe dans la première h, alors que la concentration en acide atteignit son maximum vers la troisième h. Le débit de la pepsine diminua entre la troisième et la cinquième h et augmenta légèrement entre la cinquième et la sixième h.

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This research was supported in part by grants from the National Science Foundation (No. GB 6105) and from the AMA ERF, to J. H. T. — Dr.Robitscher of Ayerst Laboratories kindly supplied the ICI-50123.     

Effects of ouabain and furosemide on saliva secretion induced by sympathomimetic agents in isolated,perfused rat submandibular glands

Summary The presence of 10^–3 M ouabain or furosemide in the perfusate inhibited saliva secretion induced by either isoproterenol (10^–5 M) or phenylephrine (10^–5 M) from isolated rat submandibular glands and caused characteristic alterations in the electrolyte composition of saliva.     

Effect of theophylline on acid secretion of stimulated human gastric mucosa

Zusammenfassung Während submaximaler und maximaler Säurestimulation mit Histalog steigert i.v. Injektion von 340 mg 7-(-Hydroxyäthyl)-theophyllin und 100 mg Theophyllin (=«OT Homburg») beim Menschen die Magensalzsäurebildung signifikant durch Zunahme der Volumenrate. Die Befunde werden im Hinblick auf die mögliche Bedeutung von zyklischem 3, 5-AMP als intrazelluläre Mittlersubstanz bei der Bildung und Sekretion von Magensalzsäure diskutiert.