微生物絮凝剂产生菌群的培养条件及絮凝特性初步研究

从活性污泥中筛选到的4株稳定的微生物絮凝剂产生菌(MBF1、MBF2、MBF3、MBF4)中构建复合菌群,选取絮凝效果最好的菌群1(MBF2和MBF3组成)进行培养条件优化,考察了培养时间、絮凝剂用量、pH等因素对絮凝效果的影响,并对复合菌群1絮凝活性分布、微生物絮凝剂热稳定性、廉价替代培养基作了初步研究。实验结果表明,复合菌群1在最佳絮凝条件下对高岭土悬浊液的絮凝率为84.86%;微生物絮凝剂分布在发酵液离心后的上清液中,具有较好的热稳定性,并能在酱油废水中生长,絮凝活性达到78.36%。     

微生物絮凝剂培养条件的研究

从活性污泥中筛选获得一株具有较高絮凝活性的絮凝剂产生菌MBFⅡ-3后,研究了培养条件的变化对其产絮凝剂的影响。研究表明,MBFⅡ-3产絮凝剂的适宜培养成份碳源最好为葡萄糖或果糖,无机盐影响不显著,蛋白胨不利于絮凝剂的产生,适宜的培养条件为pH值7.5～9,温度30℃,适量通气。MBFⅡ-3对膨润土悬液的絮凝率最高可达90.2％.     

微生物如凝剂培养条件的研究

从活性污泥中筛选获得一株具有较高絮凝活性的絮凝剂产生菌MBFⅡ-3后,研究了培养条件的变化对其产絮凝剂的研究,研究表明,MBFⅡ-产絮剂的适宜培养成份碳源最好的葡萄中果糖,无机盐影响不显著,蛋白胨不利于絮凝剂的产生,适宜的培养条件为PH值7．5－9温度30℃,t茈 。MBFⅡ-3对膨润土悬液的絮凝率最高可达90．2％。     

利用酱油废水生产微生物絮凝剂及其絮凝条件优化

为了提高微生物絮凝剂的产量,增强其絮凝效果,降低培养成本,以酱油废水替代发酵培养基对WN-2菌进行了培养,通过单因素培养条件优化,考察了外加碳源、外加氮源、培养时间、pH值等因素对微生物絮凝剂产生菌絮凝率的影响。实验结果表明,以预处理过的酱油废水作为廉价替代培养基,高效絮凝菌株WN-2可以在此条件下产生微生物絮凝剂。且WN-2最佳絮凝条件为:外加氯化钙浓度为0.2 g/L、无需添加碳源和氮源、培养时间为36 h、pH为7.0、摇床转速为160 r/min,在最佳条件下,对高岭土悬浊液进行絮凝测定,其絮凝率达到93.6%。因此,利用酱油废水作为微生物絮凝剂的替代培养基是完全可行的。     

猪粪水培养絮凝剂产生菌的研究

从活性污泥中筛选得到一株具有较高絮凝活性的絮凝剂产生的菌株FH-8,用猪粪水替代部分碳源、氮源,优化了该絮凝剂产生菌的培养条件.结果表明:在猪场养殖粪水COD质量浓度为1 000 mg/L的1 L猪粪水中添加4 g葡萄糖、0.5 g(NH4)2S)4,调节培养基初始pH值为7、培养48 h后得到的菌悬液对高岭土絮凝活性达到93.2%.用猪粪水生产的微生物絮凝剂比传统培养基生产的微生物絮凝剂更为节约成本,约为传统培养基成本的20%.     

微生物絮凝剂产生菌的筛选及其絮凝性能的实验研究

从活性污泥中分离筛选出3株对高岭土悬浊液具有较好絮凝作用的絮凝剂产生菌,通过对培养基初始pH和培养时间的优化及对采油废水絮凝处理效果的研究以了解其絮凝性能．结果表明：在培养基初始pH接近中性（pH6．0-7．0）,培养时间为12～24h条件下,3种菌株对高岭土悬浊液的絮凝效果最佳,最大絮凝率达91．5％;3种菌株对采油废水具有一定的处理效果,其絮凝率为30％～40％,CODcr去除率可达40％．     

高效微生物絮凝剂产生菌BJ-Ⅰ的筛选鉴定及絮凝特性研究

从活性污泥中筛选出1株高效微生物絮凝剂产生菌BJ-Ⅰ,其絮凝率达87．6％．通过生理生化分析及16S rDNA序列鉴定该菌株为短芽孢杆菌（Bacillus brevis）．该菌产絮凝剂的最适碳源和氮源分别为20g/L的葡萄糖和2．0g／L的蛋白胨;适宜的初始pH和培养温度分别为7．0和30℃;在培养基中添加0．5g／L的Mg^2＋有利于菌体生长,但对絮凝剂合成无影响．对该絮凝剂的特性研究结果表明：菌液投放量3％、pH值在5．9、温度为60℃条件下絮凝效果最好,加入浓度为8％的Ca^2＋可显著提高其絮凝活性．实验证明,该菌起絮凝作用的物质存在于发酵液中,主要成分为杂多糖．     

糖蜜废水培养微生物絮凝剂产生菌Pseudomonas fluorescensC-2及优化

利用糖蜜废水驯化、培养微生物絮凝荆产生茵Pseudomonas fluorescens C-2,通过单因素试验和正交试验设计优化得到该菌株产絮凝剂的最佳培养条件:培养基C:N:P为100:5:1,培养时间为48 h,培养基初始pH值8.0,糖蜜废水COD浓度8 000 mg·L-1,培养温度为30℃,摇床转速为150 r·min-1.在此培养条件下产生的絮凝剂对高岭土悬浊液絮凝率达94.75%,且对多种废水有较好的净化效果,对废水中色度和浊度的去除率均在80%以上,对COD的去除率为53.66%～85.33%,说明利用糖蜜废水培养絮凝剂产生茵C-2是完全可行的.     

Bacillus simplex利用啤酒废水产微生物絮凝剂及应用研究

利用啤酒废水培养微生物絮凝剂产生菌Bacillus simplex PS1,通过单因素试验和正交试验,优化其利用啤酒废水产生絮凝剂的培养条件;并考察所产絮凝剂对实际废水的絮凝效果。结果表明,Bacillus simplex PS1以啤酒废水为原料产生微生物絮凝剂的最佳培养条件为:啤酒废水COD浓度5 000 mg/L、KH_2PO_4 1 g/L、K_2HPO_4 2.5 g/L、初始pH值7、接种量2%(体积比)、培养温度30℃、摇床转速170 r/min、培养时间36 h。在此培养条件下,菌株所产絮凝剂对高岭土悬浊液的絮凝率达到96.8%,对城市污水、乳品废水、医院废水、淀粉废水、餐饮废水均具有良好的净化效果,对废水浊度去除率达到90%以上,对COD和色度的去除率达到80%以上,表明Bacillus simplex PS1利用啤酒废水所产微生物絮凝剂处理废水是完全可行的。     

糖蜜废水培养微生物絮凝剂产生菌Pseudomonas fluorescens C- 2及优化

利用糖蜜废水驯化、培养微生物絮凝剂产生菌Pseudomonas fluorescensC-2,通过单因素试验和正交试验设计优化得到该菌株产絮凝剂的最佳培养条件：培养基C∶N∶P为100∶5∶1,培养时间为48 h,培养基初始pH值8.0,糖蜜废水COD浓度8 000 mg·L^-1,培养温度为30℃,摇床转速为150 r·min^-1.在此培养条件下产生的絮凝剂对高岭土悬浊液絮凝率达94.75%,且对多种废水有较好的净化效果,对废水中色度和浊度的去除率均在80%以上,对COD的去除率为53.66%-85.33%,说明利用糖蜜废水培养絮凝剂产生菌C-2是完全可行的.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with